Method and solutions for cryopreserving oocytes, especially fresh human oocytes

a technology for cryopreservation and oocytes, which is applied in the field of cryopreservation oocytes, can solve the problems of low number of births of human cryopreservation oocytes, and the difficulty of cryopreservation oocytes

Inactive Publication Date: 2006-05-18
MEDI CULT AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This approach significantly improves the survival rate of cryopreserved oocytes to 82%, reducing intracellular ice formation and maintaining cellular integrity through controlled dehydration and rehydration processes.

Problems solved by technology

However, cryopreservation of oocytes, especially of fresh human oocytes, has run into greater technical difficulties than preservation of male gametes or embryos because of oocytes cytological peculiarity.
The low number of births from human cryopreserved oocytes, reported in literature, shows the technical difficulties of cryopreserving oocytes.
As yet, a definitive protocol for cryopreserving human oocytes has not been established and the number of oocytes utilised up to now is still too low to determine a definitive methodology to be applied.

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0023] The essential phases of a process, according to the present invention, for cryopreserving fresh human oocytes are described hereinafter.

[0024] The following solutions are used in the freezing process: [0025] a PBS solution (Dulbecco's Phosphate Buffered Saline Solution w / o sodium bicarbonate); [0026] an equilibration solution (1.5M PROH); [0027] a loading solution (1.5M PROH+0.3M sucrose); [0028] a SSS solution (Synthetic Serum Substitute)

[0029] The composition of the above solutions is as follows: [0030] PBS solution [0031] 8 ml PBS [0032] Equilibration solution (1.5M PROH) [0033] 6.79 ml PBS [0034] 1.21 ml PROH (1,2-propanediol) [0035] Loading solution (1.5M PROH+0.3M sucrose) [0036] 6.79 ml PBS [0037] 1.21 ml PROH [0038] 1.128 gr sucrose

[0039] These solutions are used in a slow freezing program. The solutions are prepared, mixed, filtered and conserved at +4° C. It is better to maintain the solutions at room temperature for 15 min before using.

[0040] When the above des...

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PUM

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Abstract

In a method for cryopreserving fresh human oocytes, freezing and thawing solutions are used, which solutions include 1,2 propanediol (PROH) and sucrose at a concentration of at least 0.3M. The oocytes are exposed for 13-15 minutes to a solution including 1.5M PROH and 0.3M sucrose before starting the freezing process.

Description

TECHNICAL FIELD [0001] This invention relates to an improved method for cryopreserving oocytes, especially fresh human oocytes. This invention also relates to solutions particularly suitable for cryopreservation of oocytes, especially fresh human oocytes. BACKGROUND ART [0002] It is well known that cryopreservation of unfertilised human ooccytes is a technique which offers several advantages, especially whenever oocytes are to be preserved of patients who are at risk of ovarian hyperstimulation and can not transfer embryos during the in vitro fertilization treatment. However, cryopreservation of oocytes, especially of fresh human oocytes, has run into greater technical difficulties than preservation of male gametes or embryos because of oocytes cytological peculiarity. [0003] The low number of births from human cryopreserved oocytes, reported in literature, shows the technical difficulties of cryopreserving oocytes. Up to now researches carried out on oocytes cryopreservation provid...

Claims

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Application Information

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Patent Type & AuthorityApplications(United States)
IPC IPC(8): A01N1/02
CPCA01N1/02A01N1/0221
InventorFABBRI, RAFFAELLA
OwnerMEDI CULT AS