Biomarkers associated with nephropathy

a biomarker and nephropathy technology, applied in the field of nephropathy biomarkers, can solve the problems of unsuitable invasive procedures, unsatisfactory early stage nephropathy detection, etc., and achieve the effect of reliable biomarkers

Inactive Publication Date: 2011-04-07
IND TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This approach allows for the reliable detection of early-stage nephropathy, monitoring of disease progression, and assessment of treatment efficacy, as well as renal toxicity, providing a non-invasive and sensitive diagnostic tool for both humans and laboratory animals.

Problems solved by technology

Both approaches are not suitable for detecting early stage nephropathy, which typically displays no symptoms.
While nephropathy can also be detected by renal biopsy, this invasive procedure is not an ideal diagnostic approach.

Method used

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  • Biomarkers associated with nephropathy
  • Biomarkers associated with nephropathy

Examples

Experimental program
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Effect test

example 1

Diagnosing Nephropathy Using Urine Leukocyte-Associated Ig-Like Receptor-2 or Alpha-1 Acid Glycorprotein as a Biomarker

Material and Methods

(i) Subjects

[0033]The following groups of human subjects were participated in this study:[0034](a) Healthy Donors: free of diabetic mellitus with normal renal functions,[0035](b) DM Patients: having type 2 diabetic mellitus, but free from nephropathy,[0036](c) DN Patients: having diabetic nephropathy,[0037](d) DN Uremia Patients: having DN associated with uremia,[0038](e) IgAN Patients: having IgA nephropathy,[0039](f) MGN Patients: having membranous glomerulonephritis,[0040](g) CHN Patients: having nephropathy induced by Chinese herb, and[0041](h) CIN Patients: having chronic interstitial nephritis.

Clinical characteristics of the healthy donors and the patients are summarized in Table 1 below:

TABLE 1Patient CharacteristicsHealthyDMDNDN uremiaIgANMGNCHNCINAge, mean (SD)67.9457.3372.3858.1428.3338.0047.4259.88(12.30)(10.52)(7.44)(12.79)(12.31)(13....

example 2

Diagnosing Nephropathy Using the Combination of Leukocyte-Associated Ig-Like Receptor-2 and Alpha-1 Acid Glycorprotein as a Biomarker

[0049]The levels of urine leukocyte-associated Ig-like receptor-2 and alpha-1 acid glycorprotein from both healthy controls and nephropathy patients (including patients having diabetic nephropathy, uremia, IgA nephropathy, Chinese herb-induced nephropathy, and membranous glomerulonephritis nephropathy) were determined as described in Example 1 above.

[0050]As shown in FIG. 1, the combined level of the above-mentioned two protein markers was much higher in all types of nephropathy patients as compared to that in healthy controls (AUROC=0.93). This indicates that leukocyte-associated Ig-like receptor-2 and alpha-1 acid glycorprotein, in combination, can be used as a reliable biomarker for diagnosing nephropathy with high sensitivity and specificity.

[0051]The combination of urine leukocyte-associated Ig-like receptor-2 and alpha-1 acid glycorprotein was fo...

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Abstract

Use of urine biomarkers for diagnosing nephropathy, monitoring nephropathy progress, and assessing efficacy of a nephropathy treatment. These urine biomarkers include leukocyte-associated Ig-like receptor-2, alpha-1 acid glycoprotein, their fragments, and combinations thereof.

Description

RELATED APPLICATIONS[0001]This application is a continuation of U.S. application Ser. No. 12 / 694,639, filed Jan. 27, 2010, which claims priority to U.S. Provisional Application No. 61 / 147,785, filed on Jan. 28, 2009. The contents of both prior applications are hereby incorporated by reference in their entirety.BACKGROUND OF THE INVENTION[0002]Nephropathy, commonly known as kidney damage, is caused by, among others, diabetes, high blood pressure, drug toxicity, and inflammation.[0003]Typically, nephropathy is diagnosed by determining the level of proteinuria (e.g., the level of urine albumin), or by examining the glomerular filtration rate (GFR), an indicator of renal function. Both approaches are not suitable for detecting early stage nephropathy, which typically displays no symptoms. While nephropathy can also be detected by renal biopsy, this invasive procedure is not an ideal diagnostic approach.[0004]It is of great importance to develop a method for detecting early stage nephrop...

Claims

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Application Information

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Patent Type & AuthorityApplications(United States)
IPC IPC(8): G01N33/68G01N33/53H01J49/00
CPCC07K14/70503G01N33/6893G01N2333/4728G01N2800/56G01N2800/347G01N2800/52G01N2333/70503C07K16/2803
InventorYEH, MARY YA-PINGTSENG, TZU-LINGLU, CHING-FANGLIN, WEI-YAHSU, TSAI-WEICHEN, YI-TINGYANG, CHWEI-SHIUN
OwnerIND TECH RES INST