Methods for selecting competent oocytes and competent embryos with high potential for pregnancy outcome

a technology of competent oocytes and competent embryos, applied in the field of selecting competent oocytes or competent embryos, can solve the problems of not accurately predicting the competence of embryos, the gene expression profile of cumulus cells has not been studied with respect to pregnancy outcome, and the selection of oocytes for insemination does not improve the outcome of art. , to achieve the effect of high implantation rate and high implantation ra

Inactive Publication Date: 2013-09-05
CENT HOSPITALER UNIV DE MONTPELLIER +1
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0020]The expression “a cumulus cell overexpressed a gene” means that the level of expression of said gene in the cumulus cell is higher than the expression level of said gene in a control sample.
[0051]The method as above described will thus help embryologist to avoid the transfer in uterus of embryos with a poor potential for pregnancy out come.

Problems solved by technology

However, such criteria do not truly predict the competence of an embryo.
However, a recent study has shown that the selection of oocytes for insemination does not improve outcome of ART as compared to the transfer of all available embryos, irrespective of their quality (La Sala et al., 2008).
But, despite the fact that early cleavage has been shown to be a reliable biomarker for predicting pregnancy (Lundin et al., 2001; Van Montfoort et al., 2004; Yang et al., 2007), these gene expression profiles of cumulus cells have not been studied with respect to pregnancy outcome.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods for selecting competent oocytes and competent embryos with high potential for pregnancy outcome

Examples

Experimental program
Comparison scheme
Effect test

example

A Non-Invasive Test for Assessing Embryo Potential by Gene Expression Profiles of Human Cumulus Cells

[0077]Material & Methods:

[0078]Patients and IVF Treatment:

[0079]In this retrospective study, normo-responder patients (n=30) aged of 30.9 years±2.5 and referred to our centre for ICSI (Intra Cytoplasmic Sperm Injection) for male infertility factor were studied. Patients were stimulated with a combination of GnRH agonist or antagonist with recombinant FSH (GonalF, Puregon; respectively of Merck-Serono and Organon) or with hMG (Menopur, Ferring). Ovarian response was evaluated by serum estradiol level and ultrasound examination to monitor follicle development. Retrieval of oocytes was performed 36 hours after hCG administration (5000 IU), under ultrasound guidance.

[0080]Assessment of Embryo Quality:

[0081]On day 2 and 3 postmicroinjection, the quality parameters of individually cultured embryo were evaluated using the number of blastomeres and the degree of fragmentation as criteria (gr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
volumeaaaaaaaaaa
temperatureaaaaaaaaaa
sizeaaaaaaaaaa
Login to view more

Abstract

The present invention relates to a method for selecting a competent oocyte or a competent embryo.

Description

FIELD OF THE INVENTION[0001]The present invention relates to a method for selecting a competent oocyte or a competent embryo.BACKGROUND OF THE INVENTION[0002]In assisted reproductive technology (ART), pregnancy and birth rates following in vitro fertilization (IVF) attempts remain low. Indeed, 2 out of 3 IVF cycles fail to result in pregnancy (SART 2004) and more than 8 out of 10 transferred embryos fail to implant (Kovalevsky and Patrizio, 2005). In addition, more than 50% of IVF-born babies are from multiple gestations (Reddy et al., 2007). Preterm deliveries that result from multiple pregnancies caused by ART are estimated to account for approximately $890 million of U.S. health care costs annually (Bromer and Seli, 2008).[0003]Subjective morphological parameters are still a primary criterion to select healthy embryos used for in IVF and ICSI programs. However, such criteria do not truly predict the competence of an embryo. Many studies have shown that a combination of several di...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(United States)
IPC IPC(8): C12Q1/68
CPCC12Q1/6881C12Q2600/124C12Q2600/158C12Q1/6876G01N33/689G01N33/6893G01N2800/367G01N33/5005A61B17/43C12Q1/66C12Q2600/16
Inventor HAMAMAH, SAMIRASSOU, SAIDDE VOS, JOHN
Owner CENT HOSPITALER UNIV DE MONTPELLIER
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap