Composition for preventing or treating hearing loss
a technology for hearing loss and compositions, applied in the direction of biocide, plant/algae/fungi/lichens, drug compositions, etc., can solve the problems of low effectiveness of hearing aids or cochlear implants, the majority of innate hearing loss is extremely severe in degree, and the inability to learn language, etc., to prevent or treat hearing loss, inhibit the shift of hearing threshold, and effectively inhibit the effect of hearing loss
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example 1
Preparation of Cuscuta japonica Choisy Extract
[0061]500 g of Cuscuta japonica Choisy was added to 10 L of 70% ethanol and extracted in extraction vessel at 80° C. Supernatant was collected by repeating said procedure 3 times, concentrated with vacuum concentrator (EYELA, N-N) at 45° C., and then lyophilized with ILSHIN lyophilizer at the temperature of no more than −40° C. for at least 12 hours to obtain 149.8 g of Cuscuta japonica Choisy extract.
example 2
Preparation of the Extract of Rehmannia glutinosa Libschitz var. purpurea MAKINO and Cuscuta japonica Choisy
[0062]500 g of the mixture comprising 25% of steamed Rehmannia glutinosa Libschitz var. purpurea MAKINO and 75% of Cuscuta japonica Choisy with regard to total volume was added to 10 L of 70% ethanol 10 l and extracted in extraction vessel at 80° C. Supernatant was collected by repeating said procedure 3 times, concentrated with vacuum concentrator (EYELA, N-N) at 45° C., and then lyophilized with ILSHIN lyophilizer at the temperature of no more than −40° C. for at least 12 hours to obtain 97.6 g of steamed extract of Rehmannia glutinosa Libschitz var. purpurea MAKINO and Cuscuta japonica Choisy.
example 3
Preparation of Rehmannia glutinosa Libschitz var. purpurea MAKINO and Cuscuta japonica Choisy Fraction
[0063]50 g of the extract of Rehmannia glutinosa Libschitz var. purpurea MAKINO and Cuscuta japonica Choisy obtained from above Example 2 was suspended into 1 L of distilled water and dissolved by adding 1 L of water-saturated butanol, and then only the fraction soluble in water-saturated butanol layer was separated from funnel to vacuum dry. This procedure was repeated 5 times to obtain 9.8 g of fraction.
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