Dynamic incubator system and method

a technology of incubator system and incubator chamber, which is applied in the field of system and method for dynamic incubation of cells, can solve the problems of not enabling programmable rapid changes in these conditions, not enabling design, control, implementation, etc., and achieves rapid changes in temperature

Pending Publication Date: 2022-01-27
FOREVER LABS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This approach results in reduced cell doubling time, reduced cellular senescence, and reduced inflammatory cytokine production compared to constant temperature and gas conditions, while also synergistically enhancing cell proliferation and reducing senescence when used with investigational compounds.

Problems solved by technology

However, standard incubators enable different settings in temperature or gas concentrations, but they do not enable programmable rapid changes in these conditions.
Standard incubators have a significant deficiency in that they do not enable the design, control, and implementation of dynamic temperature and gas concentrations (and consequently pH variations) over time, and thus maintain mammalian cells in conditions distinct from in vivo physiological conditions, which are not steady-state.

Method used

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  • Dynamic incubator system and method

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Embodiment Construction

[0024]Generally, a system and method of dynamically incubating cells, such as mammalian cells, is disclosed. Specifically, in vitro cell expansion can be greatly improved by adding dynamic changes in temperature, gas concentrations, and / or mechanical stress to the incubation system. A dynamic incubation system includes an incubator and a programming device communicatively coupled to the incubator. The programming device enables changes in temperature and gas concentrations (e.g., CO2 and O2) to be preprogrammed, such as having at least one preset temperature and gas concentration sequence designed and / or saved via the programming device, allowing cells to be grown in dynamic conditions. In addition, a purging mechanism, such as an air pump with an air lock, is also communicatively coupled to both the incubator and the programming device. The purging mechanism releases a portion of the gas concentration within the incubator to enable rapid changes in the temperature and gas concentra...

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Abstract

A dynamic incubator system and method for mammalian cells. The dynamic incubator system includes an incubator and a programming device communicatively coupled to the incubator. The programming device includes a memory, one or more processors, a display, and an input mechanism. The programming device is adapted to enable at least one preset temperature and gas concentration sequence to be one or more of designed for the interior of the incubator, saved to the memory of the programming device and / or selected for implementation within the interior of the incubator. The at least one temperature and gas concentration sequence includes programmed changes to the temperature and a CO2 gas concentration. A purging mechanism is coupled to the incubator and releases a portion of a gas concentration to enable rapid changes in the temperature and gas concentration in the incubator.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application claims the benefit of U.S. Provisional Application No. 62 / 816,624 filed Mar. 11, 2019. The entire contents of this application are incorporated herein by reference in their entirety.TECHNICAL FIELD[0002]This disclosure relates to cell incubator systems and methods, and in particular, to a system and method for dynamically incubating cells, such as eukaryotic cells or mammalian cells.BACKGROUND[0003]Mammalian cells are typically grown under carefully controlled conditions. These conditions vary by cell type, but generally consist of a suitable flask or dish, with a substrate or medium that supplies essential nutrients (amino acids, carbohydrates, vitamins, minerals), growth factors, hormones, and regulates pH, osmotic pressure, and temperature. Cells are grown and maintained at an appropriate temperature and gas mixture (typically, 37° C., 5% CO2 for mammalian cells) in a cell incubator. Commercially available cell incubat...

Claims

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Application Information

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Patent Type & AuthorityApplications(United States)
IPC IPC(8): C12M1/00C12M3/00C12M1/34C12M1/36
CPCC12M41/14C12M23/44C12M41/16C12M41/48C12M41/44C12M41/46C12M41/34C12M23/24C12M41/22
InventorKATAKOWSKI, MARK
OwnerFOREVER LABS INC