Concurrent monitoring of a plurality of samples by an array of biosensing elements
a biosensing element and array technology, applied in the field of sensing techniques, can solve the problems of complex series of reactions that can only exist in an intact, functioning cell, molecular recognition or chemical analysis can rarely provide this type of information, and achieve the effect of improving the detectivity of the respons
Inactive Publication Date: 2012-06-12
YISSUM RES DEV CO OF THE HEBREWUNIVERSITY OF JERUSALEM LTD
View PDF9 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
The patent is about a system and method for monitoring multiple samples simultaneously using biosensing techniques. The invention allows for the use of whole cells that have been genetically engineered to produce a measurable signal in response to specific chemical agents or physical changes in their environment. The system uses biosensors that integrate the assay substrate and sensor element into a single device, making it highly selective and efficient. The technical effect of this invention is the ability to assay global parameters such as toxicity or genotoxicity by using whole cells.
Problems solved by technology
This triggers very complex series of reactions that can exist only in an intact, functioning cell.
Molecular recognition or chemical analysis can rarely provide this type of information.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0074]FIG. 1 illustrates general principles of identifying a sample by its interaction with a microbial biosensing element, that might result in the production of a fluorescent molecule signifying the presence of the target element. Applying exciting light to the biosensor allows for detecting the fluorescence if any, and thus identifying the sample and / or the target agent.
[0075]The present invention takes advantages of the above technique, and provides a novel method and system enabling concurrent monitoring of multiple samples while interacting with multiple biosensing elements.
[0076]More specifically, the present invention utilizes optical monitoring technique applied to biosensing elements of the kind responding by fluorescence, when a fluorescent molecule is created as a result of the biosensing element interaction with a sample, and is therefore described below with respect to this specific application. It should however be noted that the invention is not limited to this speci...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
| Property | Measurement | Unit |
|---|---|---|
| electric field | aaaaa | aaaaa |
| frequency | aaaaa | aaaaa |
| phases | aaaaa | aaaaa |
Login to View More
Abstract
A system and method are presented for monitoring detectable responses from multiple biosensing elements while in contact with multiple samples located in an array. The system (1000) comprises an exciting unit (100), and a detection unit (120). The exciting unit (100) is configured for producing an array of exciting signals (102) each characterized by a frequency differing from those of the other exciting signals, and exciting a corresponding array of the biosensing elements (110), thereby enabling each biosensing element to generate a response signal (130) tagged by the different frequency. The detection unit (120) comprises a single receiving element associated with said array of biosensing elements (110) and configured for concurrently receiving the multiple response signals and generating a single output signal (140) indicative thereof. The system thereby enables to identify signal parts corresponding to the response signals of the spatially separated biosensing elements.
Description
FIELD OF THE INVENTION[0001]This invention is generally in the field of sensing techniques, and relates to a system and method for concurrent monitoring of a plurality of samples by an array of biosensing elements while in contact with the samples.REFERENCES[0002]The following references are considered to be pertinent for the purpose of understanding the background of the present invention:[0003]1. Belkin, S, 2003. Whole-cell environmental sensing of pollutants. Current Opinion Microbiol., 6: 206-212.[0004]2. Daunert, S., Barrett, G, Feliciano, J. S., Shetty, R. S., Shrestha, S. and Smith-Spencer W. 2000. Genetically engineered whole-cell sensing systems: coupling biological recognition with reporter genes. Chem. Rev. 100: 2705-2738.[0005]3. Kohler, S., S. Belkin and R. D. Schmid. 2000. Reporter gene bioassays in environmental analysis (review). Fresen. J. Anal. Chem. 366: 769-779[0006]4. Walmsley, R. M., N. Billinton and W. D. Heyer, 1997. Green fluorescent protein as a reporter fo...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(United States)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/6452G01N2021/6484
Inventor AGRANAT, AHARONBELKIN, SHIMSHON
Owner YISSUM RES DEV CO OF THE HEBREWUNIVERSITY OF JERUSALEM LTD