Application of a PCR sequencing method, based on DNA barcoding technique and DNA incomplete shearing strategy, in HLA genotyping

a pcr sequencing and dna barcoding technology, applied in the field of nucleic acid sequencing, can solve the problems of scarification of cost and throughput of first generation sequencers, and achieve the effects of enhancing hla genotyping throughput, reducing cost, and good specificity and conservation
US9957564B2Active Publication Date: 2018-05-01BGI GENOMICS CO LTD
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Patents(United States)
Current Assignee / Owner
BGI GENOMICS CO LTD
Publication Date
2018-05-01

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Abstract

The invention provides a PCR sequencing method, wherein the combination of primer indexes, DNA incomplete shearing strategy and the second generation sequencing technique (Paired-End sequencing technique) can make the length of PCR products that can be sequenced by a sequencer longer than the maximum sequencing length of the sequencer while making full use of the characteristics of the second generation sequencing technique such as high throughput and low cost, thereby greatly broadening its applicable scope. In addition, the present invention also provides primer indexes for the PCR sequencing method and the use of the method in genotyping, particularly in HLA analysis, and also provides the PCR primers used, particularly the PCR primers for HLA-A, B, HLA-C and HLA-DQB1 gene.
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Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application is a national stage application (under 35 U.S.C. § 371) of PCT / CN2011 / 076688, filed Jun. 30, 2011, claiming the priority of Chinese Patent Application No. 201010213721.2, filed Jun. 30, 2010; Chinese Patent Application No. 201010213719.5, filed Jun. 30, 2010; Chinese Patent Application No. 201010213717.6, filed Jun. 30, 2010; PCT / CN2010 / 002150, filed Dec. 24, 2010; and PCT / CN2010 / 002149, filed Dec. 24, 2010, the contents of which are incorporated herein by reference in their entirety.RELEVANT APPLICATIONS

[0002] The present application claims the priority right of the Chinese Patent Application Nos. 201010213717.6, 201010213719.5, and 201010213721.2 as filed on Jun. 30, 2010 and the priority right of the International Application Nos. PCT / CN2010 / 002150 and PCT / CN2010 / 002149 as filed on Dec. 24, 2010, the contents of which are incorporated herein by reference in their entirety.SUBMISSION OF SEQUENCE LISTING

[0003] The Sequence...

Claims

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