Calendula yellow pigment extraction method

Abstract

The invention discloses a calendula yellow pigment extraction method; the calendula or dry calendula granules are used as raw material, calendula paste is extracted by ethanol used as extractant, calendula yellow pigment which mainly contains lutein ester is extracted by a supercritical carbon dioxide extractor for paste by taking kieselguhr as dispersing agents, the extracted residue is eluted by the ethanol, and the eluent is concentrated and dried to obtain the calendula yellow pigment which mainly contains quercitrin patuletin.

Description

technical field

[0001] The invention relates to a method for extracting calendula yellow pigment. Background technique

[0002] Food coloring includes two types of synthetic coloring and natural coloring. Although chemically synthesized food coloring has bright color and good stability, it has gradually exposed its negative impact on human health, and some even have serious side effects such as carcinogenicity, such as the Sudan Red incident that has attracted much attention, and the recent related artificial coloring (sunset Yellow, tartrazine, etc.) toxicity comparable to leaded gasoline, seriously affecting children's health reports, once again sounded the alarm for human use of synthetic pigments. Natural pigments have attracted the attention of many countries due to their unique advantages such as low toxic and side effects and certain health and dietary effects on the human body. At present, all countries are actively committed to the research and development of plan...

Examples

Embodiment 1

[0009] Add 5000mL of 95% industrial ethanol to 400g of calendula dried flower granules, ultrasonicate for 60min (ultrasonic 4s, interval 1s, power 1100W, rotating speed 1100 rpm), and extract twice to obtain 134.8g of extract.

[0010] Supercritical conditions: Instrument model: HA121-50-01, Jiangsu Nantong Instrument Factory, pressure 45.0MPa, temperature 47°C, extraction time: 130min, CO 2 Flow rate: F16.0ml / min, extract once.

[0011] Take 20g of the dried flower granule extract extracted by ultrasonic circulation, mix the sample with 180g of diatomaceous earth and carry out supercritical extraction to obtain 7g of marigold yellow pigment mainly containing lutein ester, and use 95% industrial ethanol to ultrasonically elute the diatomite From the residue, 8.64 g of calendula yellow pigment mainly containing quercetin was obtained.

Embodiment 2

[0013] Calendula dried flower granules are replaced with marigold, and other conditions are the same as in Example 1, and 6.8 g of marigold yellow pigment mainly containing lutein esters and 8.3 g of marigold yellow pigment mainly containing quercetin tagetine are obtained. g.

technical field

[0001] The invention relates to a method for extracting calendula yellow pigment. Background technique

[0002] Food coloring includes two types of synthetic coloring and natural coloring. Although chemically synthesized food coloring has bright color and good stability, it has gradually exposed its negative impact on human health, and some even have serious side effects such as carcinogenicity, such as the Sudan Red incident that has attracted much attention, and the recent related artificial coloring (sunset Yellow, tartrazine, etc.) toxicity comparable to leaded gasoline, seriously affecting children's health reports, once again sounded the alarm for human use of synthetic pigments. Natural pigments have attracted the attention of many countries due to their unique advantages such as low toxic and side effects and certain health and dietary effects on the human body. At present, all countries are actively committed to the research and development of plan...

Examples

Embodiment 1

[0009] Add 5000mL of 95% industrial ethanol to 400g of calendula dried flower granules, ultrasonicate for 60min (ultrasonic 4s, interval 1s, power 1100W, rotating speed 1100 rpm), and extract twice to obtain 134.8g of extract.

[0010] Supercritical conditions: Instrument model: HA121-50-01, Jiangsu Nantong Instrument Factory, pressure 45.0MPa, temperature 47°C, extraction time: 130min, CO 2 Flow rate: F16.0ml / min, extract once.

[0011] Take 20g of the dried flower granule extract extracted by ultrasonic circulation, mix the sample with 180g of diatomaceous earth and carry out supercritical extraction to obtain 7g of marigold yellow pigment mainly containing lutein ester, and use 95% industrial ethanol to ultrasonically elute the diatomite From the residue, 8.64 g of calendula yellow pigment mainly containing quercetin was obtained.

Embodiment 2

[0013] Calendula dried flower granules are replaced with marigold, and other conditions are the same as in Example 1, and 6.8 g of marigold yellow pigment mainly containing lutein esters and 8.3 g of marigold yellow pigment mainly containing quercetin tagetine are obtained. g.