Complex microbial agent for producing soybean paste in Pixian County and preparation method thereof

Abstract

The invention discloses a complex microbial agent for producing soybean paste in Pixian County, which mainly comprises coculture of saccharomyces cerevisiae, candida utilis, lactobacillus plantarum and salt tolerant tetrads, and skim milk powder, glycerin, maltodextrin, trehalose and L-sodium glutamate, or mainly comprises coculture of saccharomyces cerevisiae, candida utilis, lactobacillus plantarum and salt tolerant tetrads, and porous starch, maltodextrin and polyvinyl pyrrolidone. A method for preparing the complex microbial agent for producing soybean paste in Pixian County comprises thefollowing technological steps of: (1) activating and propagating strains; (2) inoculating; (3) co-culturing; and (4) adding a protective agent and drying. By using the complex microbial agent in the preparation of cooked soybean paste, the complex microbial agent maintains the characteristics of traditional soybean paste in Pixian County. Meanwhile, compared with the traditional technology, the complex microbial agent has the advantages of shortening the production period by 1 / 3, improving the amino nitrogen content by 3 to 8 times, improving the volatile aroma content by 2 to 4 times, and ensuring the aflatoxin B1 of 0 to 0.5ppm only.

Description

technical field

[0001] The invention belongs to the field of compound microbial bacterial agents, and in particular relates to a compound microbial bacterial agent for watercress production and a preparation method thereof. Background technique

[0002] Pixian Douban has the reputation of "the soul of Sichuan cuisine" and has a history of more than 300 years. Pixian Douban is brewed with red pepper and broad beans as the main raw materials, edible salt, wheat flour, etc., according to traditional techniques. Douban. Pixian Douban is produced in Pixian County, Chengdu City, Sichuan Province, my country. It is a geographical indication product approved by the General Administration of Quality Supervision, Inspection and Quarantine. Traditional Pixian Douban relies on the natural inoculation of microorganisms in the environment. In recent years, most Pixian Douban production enterprises have begun to inoculate with Aspergillus oryzae 3.042, which is used for soy sauce product...

Examples

Embodiment 1

[0046] The processing steps of the present embodiment are as follows:

[0047] (1) Activation and expansion of strains

[0048] ●Strain activation

[0049] Put 10mL of normal saline with a mass concentration of 0.9% into 4 test tubes, sterilize at 121°C for 20 minutes, and then cool to 30°C. 1g each of Bacillus freeze-dried bacteria powder and halo-tolerant Tetradococcus freeze-dried bacteria powder were added to 4 test tubes under sterile conditions, shaken and dissolved, and placed in a 30°C constant temperature incubator to activate for 30 minutes for later use;

[0050] ●Expanded culture of bacteria

[0051] Insert the activated Lactobacillus plantarum and Halotolerance Tetradendococcus respectively into the bacterial expansion medium, and cultivate them on a shaker (130r / min) at 30°C for 21h; the volume percentage of the Lactobacillus plantarum bacterial solution is 1%, and the The volume percentage of said bacteria expansion medium is 99%; the volume percentage of sai...

Embodiment 2

[0070] The processing steps of the present embodiment are as follows:

[0071] (1) Activation and expansion of strains

[0072] ●Strain activation

[0073] Put 10mL of normal saline with a mass concentration of 0.9% into 4 test tubes, sterilize at 121°C for 20 minutes, and then cool to 30°C. 0.5g each of Bacillus freeze-dried bacteria powder and halo-tolerant Tetradococcus freeze-dried bacteria powder were added to 4 test tubes under sterile conditions, shaken and dissolved, and placed in a 30°C constant temperature incubator to activate for 40 minutes for later use;

[0074] ●Expanded culture of bacteria

[0075] Insert the activated Lactobacillus plantarum and tetradococcus halotolerant respectively into the bacterial expansion medium, and cultivate them in a shaker (180r / min) at 30°C for 24h; the volume percentage of the Lactobacillus plantarum bacterial solution is 2%, and the The volume percentage of said bacteria expansion medium is 98%; the volume percentage of said ...

technical field

[0001] The invention belongs to the field of compound microbial bacterial agents, and in particular relates to a compound microbial bacterial agent for watercress production and a preparation method thereof. Background technique

[0002] Pixian Douban has the reputation of "the soul of Sichuan cuisine" and has a history of more than 300 years. Pixian Douban is brewed with red pepper and broad beans as the main raw materials, edible salt, wheat flour, etc., according to traditional techniques. Douban. Pixian Douban is produced in Pixian County, Chengdu City, Sichuan Province, my country. It is a geographical indication product approved by the General Administration of Quality Supervision, Inspection and Quarantine. Traditional Pixian Douban relies on the natural inoculation of microorganisms in the environment. In recent years, most Pixian Douban production enterprises have begun to inoculate with Aspergillus oryzae 3.042, which is used for soy sauce product...

Examples

Embodiment 1

[0046] The processing steps of the present embodiment are as follows:

[0047] (1) Activation and expansion of strains

[0048] ●Strain activation

[0049] Put 10mL of normal saline with a mass concentration of 0.9% into 4 test tubes, sterilize at 121°C for 20 minutes, and then cool to 30°C. 1g each of Bacillus freeze-dried bacteria powder and halo-tolerant Tetradococcus freeze-dried bacteria powder were added to 4 test tubes under sterile conditions, shaken and dissolved, and placed in a 30°C constant temperature incubator to activate for 30 minutes for later use;

[0050] ●Expanded culture of bacteria

[0051] Insert the activated Lactobacillus plantarum and Halotolerance Tetradendococcus respectively into the bacterial expansion medium, and cultivate them on a shaker (130r / min) at 30°C for 21h; the volume percentage of the Lactobacillus plantarum bacterial solution is 1%, and the The volume percentage of said bacteria expansion medium is 99%; the volume percentage of sai...

Embodiment 2

[0070] The processing steps of the present embodiment are as follows:

[0071] (1) Activation and expansion of strains

[0072] ●Strain activation

[0073] Put 10mL of normal saline with a mass concentration of 0.9% into 4 test tubes, sterilize at 121°C for 20 minutes, and then cool to 30°C. 0.5g each of Bacillus freeze-dried bacteria powder and halo-tolerant Tetradococcus freeze-dried bacteria powder were added to 4 test tubes under sterile conditions, shaken and dissolved, and placed in a 30°C constant temperature incubator to activate for 40 minutes for later use;

[0074] ●Expanded culture of bacteria

[0075] Insert the activated Lactobacillus plantarum and tetradococcus halotolerant respectively into the bacterial expansion medium, and cultivate them in a shaker (180r / min) at 30°C for 24h; the volume percentage of the Lactobacillus plantarum bacterial solution is 2%, and the The volume percentage of said bacteria expansion medium is 98%; the volume percentage of said ...