Preparation method of efficient composite micro-ecologic premix for animals

A composite microecological and premix technology, which is applied in the preparation of veterinary high-efficiency composite microecological premix and the field of microecological premix preparation, can solve the problem that high temperature processing and storage process are unstable and affect the microecological premix. In order to improve animal production performance, improve feed utilization, and reduce body stress

CN103202393AInactive Publication Date: 2013-07-17SICHUAN HUANYA BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2013-07-17
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention relates to a preparation method of an efficient composite micro-ecologic premix for animals, and belongs to the technical field of micro-ecologic preparation feed additives. The preparation method comprises the following steps: a step of respective optimal screening of Bacillus licheniformis, Bacillus subtilis, Candida utilis and Saccharomyces cerevisiae, a step of bacterial strain rejuvenation, a step of shaking culture, a step of culturing in a 30L fermentation cylinder, a step of thallus spray drying, and a step of preparing composite bacterial powder. The efficient composite micro-ecologic premix prepared through the method has the characteristics of excellent acid, salt, thermal and bile resistances, high content of live bacteria, high-copper tolerance, and good stability under feed granulation processing and normal temperature preservation conditions, the preparation method of the premix is simple and has a low cost, and the premix product produced through the method has a stable and reliable quality, and has a good market prospect.
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Description

technical field

[0001] The invention relates to a preparation method of a microecological premix, more specifically to a preparation method of a high-efficiency compound microecological premix for animals, and belongs to the technical field of probiotic feed additives. Background technique

[0002] Veterinary micro-ecological premix is ​​a natural, green and safe feed additive. Due to its advantages of non-toxic, harmless, no residue and no pollution, it can prevent and treat diseases, improve feed utilization, and improve animal health. Production performance and other effects, and can overcome the bacterial flora imbalance, superinfection and drug resistance caused by antibiotics, and have been widely studied and applied in the case of increasingly prominent animal-derived food safety problems.

[0003] At present, the veterinary microecological premix products on the domestic market mainly have the following deficiencies: (1) The content of live bacteria is low. 7 1 / g, t...

Examples

Embodiment 1

[0037] A preparation method for a high-efficiency compound microecological premix for animals, comprising the following steps:

[0038] 1) Strain optimization screening

[0039] The strains of Bacillus licheniformis, Bacillus subtilis, Candida utilis and Saccharomyces cerevisiae were respectively acclimated to acid resistance, bile salt resistance, temperature resistance and high copper resistance;

[0040] The acid-resistant acclimatization refers to the selection of acid-resistant bacterial strains under the condition of pH 3.2-3.6;

[0041] The bile-salt-resistant acclimatization refers to: after adding bile-salts to the culture medium, the selected acid-resistant bacterial strains are acclimatized;

[0042] The temperature-resistant acclimation refers to: inoculating the bacterial cultures acclimatized to bile salts into LB and TB liquid medium, then heating in a water bath at 40-60°C, and then continuing to culture the culture at 37°C;

[0043] The high-copper-resistant...

Embodiment 2

[0062] A preparation method for a high-efficiency compound microecological premix for animals, comprising the following steps:

[0063] 1) Strain optimization screening

[0064] The strains of Bacillus licheniformis, Bacillus subtilis, Candida utilis and Saccharomyces cerevisiae were respectively acclimated to acid resistance, bile salt resistance, temperature resistance and high copper resistance;

[0065] The acid-resistant acclimatization refers to the selection of acid-resistant bacterial strains under the condition of pH 3.2-3.6;

[0066] The bile-salt-resistant acclimatization refers to: after adding bile-salts to the culture medium, the selected acid-resistant bacterial strains are acclimatized;

[0067] The temperature-resistant acclimation refers to: inoculating the bacterial cultures acclimatized to bile salts into LB and TB liquid medium, then heating in a water bath at 40-60°C, and then continuing to culture the culture at 37°C;

[0068] The high-copper-resistant...

Embodiment 3

[0087] A preparation method for a high-efficiency compound microecological premix for animals, comprising the following steps:

[0088] 1) Strain optimization screening

[0089] The strains of Bacillus licheniformis, Bacillus subtilis, Candida utilis and Saccharomyces cerevisiae were respectively acclimated to acid resistance, bile salt resistance, temperature resistance and high copper resistance;

[0090] The acid-resistant acclimatization refers to the selection of acid-resistant bacterial strains under the condition of pH 3.2-3.6;

[0091] The bile-salt-resistant acclimatization refers to: after adding bile-salts to the culture medium, the selected acid-resistant bacterial strains are acclimatized;

[0092] The temperature-resistant acclimation refers to: inoculating the bacterial cultures acclimatized to bile salts into LB and TB liquid medium, then heating in a water bath at 40-60°C, and then continuing to culture the culture at 37°C;

[0093] The high-copper-resistant...