Clostridium butyricum and its application

A technology of Clostridium butyricum and strains, applied in the fields of application, bacteria, microorganisms, etc., can solve the problems of few research reports on Clostridium butyricum and less application research, so as to improve animal production performance, improve the structure of flora, The effect of reducing usage

CN104164377AActive Publication Date: 2014-11-26ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2014-11-26

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Abstract

The invention discloses a Clostridium butyricum and its application. The Clostridium butyricum is Clostridium butyricum ZJU-F1 specifically, is separated from the intestinal tract of a healthy piglet, and has strong stress resistance and high temperature resistance. A fermentation product is emulsified to prepare a Clostridium butyricum preparation, and the preparation can used for preparing animal feeds as a feed additive, and can substitute antibiotics or / and reduce the use of zinc oxide. The above micro-ecological preparation can regulate the micro-ecological balance in the intestinal tracts of animals, promotes the growth of beneficial bacteria, enhances the nonspecific immune function of animals, secretes trophic factors and protease, promote s the digestion and absorption of nutrients, and promotes animal production to improve the feed pay.
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Description

technical field

[0001] The invention relates to the field of feed additive-microbial preparations, in particular to a high-efficiency and safe Clostridium butyricum and its application. Background technique

[0002] Feed antibiotics have significant effects in promoting animal growth, improving feed conversion efficiency, reducing food loss, and increasing pork production. However, with the widespread use of antibiotics as animal growth-promoting agents in feed, its disadvantages are becoming increasingly prominent, such as causing animal gastrointestinal flora imbalance, drug resistance and drug residues, etc., causing a series of public safety and environmental issues. Conservation and livestock product quality issues. It has become one of the hotspots in the field of animal nutrition to search for green and safe feed additives that are non-toxic, efficient, and do not produce drug resistance.

[0003] Feed microbial preparations are beneficial to balance the intestinal ...

Examples

Embodiment example 1

[0031] Implementation Case 1 Isolation and Identification of Strains

[0032] 1. Experimental method

[0033] 1.1 Experimental materials 10 copies of healthy piglet feces from a pig farm in Zhejiang Province

[0034] 1.2 Medium

[0035] ①Clostridium proliferation medium (both mass concentration g / L): tryptone 20, beef extract 10, yeast extract 6, glucose 4, taurocholate 5, compound salt 1 part (K 2 HPO 4 2. KH 2 PO 4 1. MgSO 4 0.4, CaCl 2 0.2, FeSO 4 0.1, cysteine ​​hydrochloride 0.5, the same below); pH7.2, 121℃, 15min sterilization

[0036] ②Clostridium count medium (both mass concentration g / L): tryptone 10, beef extract 5, yeast extract 3, glucose 4, agar 16; one part of compound salt, pH 7.2, sterilized at 121°C for 15 minutes

[0037] ③Clostridium culture medium (both mass concentration g / L): tryptone 20, beef extract 10, yeast extract 6, glucose 4, compound salt 1 part; pH 7.2, 121 ° C, 15 min sterilization

[0038] 1.3 Specific experimental methods

[0039] ...

Embodiment example 2

[0059] Application of Clostridium butyricum ZJU-F1 and its microbial compound preparation instead of antibiotics to reduce zinc oxide in weaned piglets

[0060] Purpose and Significance: Weaned piglets were selected as the test object, and the effects of Clostridium butyricum microecological preparations on the growth performance, diarrhea rate, and intestinal microecological flora of weaned piglets were investigated under the condition of no antibiotics and low dose of zinc oxide, aiming to study Effects of probiotics on growth performance, weaning stress relief, and gut microbiota in reducing antibiotic and high-dose zinc oxide use.

[0061] Experimental animals: select 120 healthy weaned piglets of 23±2 days old Du×Long×Da Sanyuan crossbreeding, with a body weight of 7.8±0.2Kg.

[0062] Clostridium butyricum preparation: Clostridium butyricum (Clostridium butyricum) ZJU-F1 liquid fermentation and spray drying, corn flour as carrier, the number of viable bacteria in the prep...