A method for extracting total flavonoids from peony

A technology of peony flower and total flavonoids, which is applied in the field of plant biology, can solve the problems of long action time, waste of energy, and affect the extraction effect, and achieve the effects of mild extraction conditions, increased mass transfer rate, and improved extraction rate

CN103520301BActive Publication Date: 2016-01-20洛阳祥和牡丹科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2016-01-20
Patent Text Reader

Abstract

A method for extracting total flavonoids from peony flowers. The invention belongs to the technical field of plant biology, and in particular relates to a method for extracting peony essential oil from peony flowers. It includes picking, preparing compound enzyme solution, ultrasonic treatment, centrifugal treatment and vacuum distillation. Enzyme treatment of peony flowers at 35°C for 20 minutes can cause local loosening, expansion, and collapse of the cell wall and interstitial structure of peony flowers; Strong shock waves and micro jets are generated inside the liquid. This powerful shock flow can effectively reduce and eliminate the barrier layer between the solvent and the water phase, thereby increasing the mass transfer rate. A physical shear force is generated to deform, rupture, and release the contents, resulting in local loosening and swelling of the cell wall and its interstitial structure, which greatly accelerates the extraction process of flavonoids in the cell and plays a role Get twice the result with half the effort.
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Description

Technical field

[0001] The present invention belongs to the field of plant biotechnology, which involves a method of extracting total flavonoids from peony flowers. Background technique

[0002] Total flavonoids are the general name of flavonoids. It has the effects of antioxidant clearance free radicals, expanded cardiovascular, and trace estrogen -like, and can also reduce the incidence of prostate cancer, colon cancer and other diseases such as myocardial infarction, atherosclerosis and other diseases.And mortality.At the same time, flavonoids are also important functional food additives, feed additives, natural antioxidants, and have a skin care and beauty effect.Therefore, flavonoids have a positive effect on prevention and treatment of diseases and health, and have broad market potential.

[0003] The peony belongs to the rock of the family, and it is known as the beauty of Luoyang flowers, rich flowers and the "king of flowers".In addition to its root skin is an important ...

Examples

Embodiment 1

[0025] A method for extracting total flavonoids from peony flowers, comprising the following steps:

[0026] Step 1. Picking mature and disease-free peony flowers, placing the peony flowers in an oven with a temperature of 27°C and blowing them to dry, then taking out the peony flowers and crushing them to 40 meshes for later use;

[0027] Step 2. Mix the cellulase aqueous solution with a mass concentration of 0.2 mg / mL and the pectinase aqueous solution with a mass concentration of 0.1 mg / mL in equal volumes, and add an acetic acid solution with a mass percentage concentration of 2% to the mixed solution until mixed When the pH value of the solution reaches 5.0, stir the mixed solution evenly and let it stand for 12 minutes to obtain a compound enzyme solution;

[0028] Step 3, put the peony flower obtained in step 1 into the triangular flask, add the compound enzyme liquid prepared in step 2 in the conical flask, the ratio of compound enzyme liquid and peony flower a...