Application of soybean iron metabolism related gene GmIMD

Abstract

The invention provides an application of a soybean iron metabolism related gene GmIMD, wherein the application at least includes improvement of the utilization rate of iron elements of plants and improvement of the chlorophyll content in plant leaves. The invention discloses a biological function of the GmIMD gene for the first time, wherein the gene is a gene participating in the iron metabolismprocess in the plants, especially in the regulation of the iron content involved during soybean development. Valuable genetic resources are provided for promoting the in-depth study of iron metabolismin the plants and cultivating new varieties of high-quality soybean with high-efficiency utilization of iron.

Description

technical field

[0001] The invention relates to the fields of biotechnology and plant genetic breeding, in particular to the application of soybean iron metabolism-related gene GmIMD. Background technique

[0002] Iron plays a very important role in the life process of organisms and is an essential mineral element for the survival of organisms. Although iron is abundant in the Earth's crust, it is very inefficiently utilized by living organisms. Leguminous crops such as soybeans are most prone to iron deficiency, because iron is a component of heme and nitrogenase. Iron deficiency in plants weakens the nitrogen fixation of rhizobia, resulting in short plants, yellowing of upper leaves between veins, and slight curling. When the new leaves are yellowish white, many brown spots appear near the edge of the leaves, and then necrosis. Due to the low efficiency of plants to absorb and utilize iron from external sources, it cannot be solved by fertilization at present, but can on...

Examples

Embodiment 1

[0025] Example 1 Construction and Phenotype Analysis of Gmimd Mutant Population

[0026] Through the screening of the "Hedou 12" soybean gamma radiation mutation mutant library, after 4 generations of mutant planting, phenotype observation and statistical analysis, a Gmimd mutation with stable inheritance and Mendelian inheritance (3:1 segregation ratio) was obtained. body. The F2 segregation population for map-based cloning was constructed by crossing with the distant species Williams82. The phenotype observation results showed that compared with the wild type 'Hedou 12', the leaves of the Gmimd mutant showed obvious phenotypic changes of edge yellowing, albinism and spot chlorosis ( figure 1 ).

Embodiment 2

[0027] Example 2 Location of Soybean Iron Metabolism-Related Gene GmIMD

[0028] Gene mapping was performed using the F2 segregant population of Gmimd and Williams82. The rough mapping results showed that the mutation site was located between 5.2Mb and 11.4Mb on chromosome 2. In order to further determine the location of the Gmimd mutant gene, new Indel molecular markers and SNP molecular markers were designed in the region between 5.2Mb and 11.4Mb of chromosome 2 to fine-map the mutant individual plants, and finally the Gmimd mutant gene was located at The 170kb interval between 7.29Mb-7.46Mb of chromosome 2 ( figure 2 ). In this 170kb interval, there are a total of 20 candidate genes. Through the whole genome resequencing of the Gmimd mutant, it was found that except for the base mutation of the GmIMD gene, the genome sequences of other genes were completely consistent with the original genome sequence. Therefore, we inferred that the phenotypes of edge yellowing, albini...

Embodiment 3

[0029] The mensuration of embodiment 3 Gmimd mutant chlorophyll content

[0030] By measuring the chlorophyll content of wild-type 'Hedou 12' and Gmimd mutant leaves, it was found that there were significant differences in chlorophyll a, chlorophyll b and total chlorophyll content between wild-type 'Hedou 12' and Gmimd mutant. Compared with the wild-type chlorophyll a, chlorophyll b, the total chlorophyll content of the mutant decreased by 60.91%, 54.93%, 59.63% ( image 3 ). Therefore, we speculate that the mutation of this gene may lead to the blockage of chlorophyll synthesis in soybean leaves, resulting in a decrease in chlorophyll content of soybean mutant leaves.

technical field

[0001] The invention relates to the fields of biotechnology and plant genetic breeding, in particular to the application of soybean iron metabolism-related gene GmIMD. Background technique

[0002] Iron plays a very important role in the life process of organisms and is an essential mineral element for the survival of organisms. Although iron is abundant in the Earth's crust, it is very inefficiently utilized by living organisms. Leguminous crops such as soybeans are most prone to iron deficiency, because iron is a component of heme and nitrogenase. Iron deficiency in plants weakens the nitrogen fixation of rhizobia, resulting in short plants, yellowing of upper leaves between veins, and slight curling. When the new leaves are yellowish white, many brown spots appear near the edge of the leaves, and then necrosis. Due to the low efficiency of plants to absorb and utilize iron from external sources, it cannot be solved by fertilization at present, but can on...

Examples

Embodiment 1

[0025] Example 1 Construction and Phenotype Analysis of Gmimd Mutant Population

[0026] Through the screening of the "Hedou 12" soybean gamma radiation mutation mutant library, after 4 generations of mutant planting, phenotype observation and statistical analysis, a Gmimd mutation with stable inheritance and Mendelian inheritance (3:1 segregation ratio) was obtained. body. The F2 segregation population for map-based cloning was constructed by crossing with the distant species Williams82. The phenotype observation results showed that compared with the wild type 'Hedou 12', the leaves of the Gmimd mutant showed obvious phenotypic changes of edge yellowing, albinism and spot chlorosis ( figure 1 ).

Embodiment 2

[0027] Example 2 Location of Soybean Iron Metabolism-Related Gene GmIMD

[0028] Gene mapping was performed using the F2 segregant population of Gmimd and Williams82. The rough mapping results showed that the mutation site was located between 5.2Mb and 11.4Mb on chromosome 2. In order to further determine the location of the Gmimd mutant gene, new Indel molecular markers and SNP molecular markers were designed in the region between 5.2Mb and 11.4Mb of chromosome 2 to fine-map the mutant individual plants, and finally the Gmimd mutant gene was located at The 170kb interval between 7.29Mb-7.46Mb of chromosome 2 ( figure 2 ). In this 170kb interval, there are a total of 20 candidate genes. Through the whole genome resequencing of the Gmimd mutant, it was found that except for the base mutation of the GmIMD gene, the genome sequences of other genes were completely consistent with the original genome sequence. Therefore, we inferred that the phenotypes of edge yellowing, albini...

Embodiment 3

[0029] The mensuration of embodiment 3 Gmimd mutant chlorophyll content

[0030] By measuring the chlorophyll content of wild-type 'Hedou 12' and Gmimd mutant leaves, it was found that there were significant differences in chlorophyll a, chlorophyll b and total chlorophyll content between wild-type 'Hedou 12' and Gmimd mutant. Compared with the wild-type chlorophyll a, chlorophyll b, the total chlorophyll content of the mutant decreased by 60.91%, 54.93%, 59.63% ( image 3 ). Therefore, we speculate that the mutation of this gene may lead to the blockage of chlorophyll synthesis in soybean leaves, resulting in a decrease in chlorophyll content of soybean mutant leaves.

Images