16 results about "Chrysophanic acid" patented technology

The invention relates to the technical field of biological medicines, and provides a self-assembled polypeptide-rhein conjugate, a preparation as well as a preparation method and application of the self-assembled polypeptide-rhein conjugate. The sequence of the MMP-9 responsive self-assembled polypeptide-rhein conjugate is Rhein-GFFPLGLAGACE, and the MMP-9 responsive self-assembled polypeptide-rhein conjugate can respond to abnormally activated MMP-9 in atherosclerotic plaque and is self-assembled into a stable nanofiber structure in situ at a diseased region; the anti-inflammatory and anti-oxidation functions of rhein in the conjugate are remarkable, but the water solubility and oral bioavailability are low, the anti-inflammatory and anti-oxidation functions are remarkably improved after being coupled with the polypeptide, targeted delivery is achieved, and the residence time is prolonged; the introduction of the tripeptide ACE simulating the function of glutathione further enhances the oxidative stress resistance of the conjugate. Furthermore, the conjugate shows excellent anti-inflammatory and anti-oxidation effects, can clear ROS, inhibit inflammation pathways, regulate macrophage phenotypes and inhibit apoptosis, and provides a new strategy for treatment of atherosclerosis.

The present application relates to the technical field of bacteriostatic drugs, and particularly relates to application of traditional Chinese medicine monomers in preparation of bacterial efflux pump AcrB inhibitor or antibiotic activity improving drug and combined bacteriostatic composition. The traditional Chinese medicine monomers include red cedar flavone, stigmasterol, trichosanthin diol-3-benzoic acid ester, glycyrrhizic acid, alpha-bosanol, agastachin, acaciin, triptonylline C, rhein acid diglucoside, brassicasterol ferulate, cholesterin ferulate or calophyllolide G. The traditional Chinese medicine monomers can inhibit the efflux activity of AcrB, so that the drug molecules entering the bacteria continuously accumulate, thereby recovering the bactericidal efficiency of the existing antibacterial drugs and reducing the drug resistance of bacteria to the antibacterial drugs.

The invention provides a method for detecting the blood concentration of Shenfukang capsules. The method comprises the following steps: respectively preparing a first detection specimen containing a serum sample and a second detection specimen containing a urine sample; the method comprises the following steps: taking a first sample to be detected and a second sample to be detected, injecting a Supersil ODS 2 chromatographic column, taking 0.1% phosphoric acid aqueous solution-methanol as a mobile phase, respectively detecting the chromatographic peak of calycosin in the first sample to be detected and the chromatographic peak of rhein in the second sample to be detected, and calculating the concentration of calycosin and the concentration of rhein in the samples according to the chromatographic peaks; the chromatographic conditions of the first detection sample are that the detection wavelength is 260 nm, and gradient elution is carried out; the chromatographic conditions of the second detection sample are as follows: the detection wavelength is 254 nm, and isocratic elution is performed. The method has excellent repeatability and high sensitivity, the operation process is simple and easy to implement, the administration scheme can be accurately adjusted and an individualized administration strategy can be implemented in close combination with clinical manifestation and various inspection results of a patient, so that the safety and effectiveness of the medication process are ensured, and the method is suitable for clinical popularization and application. And a reliable medication guarantee is provided for the treatment of patients with chronic kidney diseases.

The invention discloses preparation and application of a nano enzyme. The preparation method of the nano enzyme comprises the following steps: dissolving copper salt and hydroxy anthraquinone carboxylic acid in water to obtain a first solution; adjusting the pH of the first solution to be alkaline, and heating for reaction to obtain a product solution; and centrifuging the product solution, taking the precipitate, washing and drying to obtain the Cu nano-enzyme. The copper salt comprises at least one of copper sulfate, copper chloride, copper nitrate and copper hydroxide; the hydroxy anthraquinone carboxylic acid is rhein. According to the invention, Cu is used as a transition metal source and reacts with specific hydroxy anthraquinone carboxylic acid to prepare the Cu nano-enzyme (Cu-Rhein) with excellent peroxidase-like activity. The Cu nano enzyme can be applied to promotion of hydrogen peroxide oxidation TMB color development and detection of penicillamine, the accuracy of penicillamine detection is high, and the Cu nano enzyme has good application prospects.

The invention belongs to the field of medicines, and particularly relates to a quality detection method of a clearing and deacidification mixture. According to the invention, a characteristic spectrum method for simultaneously detecting 10 main active components (paeoniflorin, puerarin, gentiopicroside, loganic acid, astilbin, benzoylpaeoniflorin, resveratrol, rhein, rhizoma dioscoreae septemlobae A and rhizoma dioscoreae septemlobae B) in the mixture is established for the first time, the limitation of single component quality control is overcome, and the overall quality of a medicine can be evaluated more comprehensively and objectively; by optimizing a sample pretreatment process and chromatographic gradient elution conditions, interference of a complex matrix is effectively eliminated, and good separation of each component is ensured. The method is high in specificity, negative, free of interference and capable of accurately recognizing target components.

The invention discloses a colon-targeted Pickering emulsion stabilized by rhein-chitosan composite nanoparticles, a preparation method and an application of the colon-targeted Pickering emulsion. Rhein-chitosan is coupled and then crosslinked with sodium tripolyphosphate to form the rhein-chitosan composite nano-particles, and the nano-particles have better hydrophobicity and acid and alkali resistance stability; the nano-particle suspension and fish oil containing rhein are homogenized and emulsified at a high speed to obtain the Pickering emulsion entrapped with the rhein, and the Pickering emulsion has relatively high rhein drug loading capacity, better stability and good colon-specific release and slow release characteristics; and the traditional Chinese medicine composition has a dose-dependent relieving effect on dextran sodium sulfate induced ulcerative colitis mice, can inhibit inflammatory response and repair intestinal barrier functions, can effectively avoid intestinal nutrition loss caused by rhein, and has a good application prospect.

The invention provides a rheum officinale active ingredient composition as well as a preparation method and application thereof, and the active ingredients of the composition consist of emodin methyl ether glycoside, rhein, rhein, gallic acid, emodin and chrysophanol glycoside. The weight ratio of physcion glycoside to rhein glycoside to rhein to gallic acid to emodin to chrysophanol glycoside is 200: 100: 20: 10: 1: 1, the composition is prepared in a monomer mixing mode, the pancreatitis focus area is remarkably reduced, the pathological score is remarkably improved, and the serum amylase and lipase level is remarkably reduced; a certain liver protection effect is achieved; the components are clear in material basis, have no obvious liver and kidney toxicity, and can obviously improve and treat pancreatitis.

The application belongs to the technical field of content detection, and discloses a kind of HPLC detection methods for simultaneously determining the content of multiple components in semen Cannabis capsules.The detection method includes mixing reference solution, chrysophanol and methanol to obtain mixed reference solution.The reference solution includes physcion solution, magnolol solution, houpo chrysophanol solution, emodin solution, rhein solution, nobiletin solution, aloe emodin solution, hesperidin solution and paeoniflorin solution;The contents of semen Cannabis capsules are mixed with methanol, and the test solution is obtained after heating and refluxing;The mixed reference solution and test solution are respectively sucked and injected into high performance liquid chromatograph.The detection method can simultaneously determine the content of multiple index components, can comprehensively reflect the quality of the preparation, and the test method has excellent precision, repeatability, stability and high sample recovery rate.

The invention discloses a quality control method and a preparation process optimization method of Yulingling ointment, which are characterized in that three key components for benefiting qi and nourishing blood, namely 6-Paradol (zingiberol), Eupafolin (eupatorin) and Chrysophanic Acid (chrysophanol), are screened by utilizing a network pharmacology technology and molecular docking analysis; and then quality control and preparation process optimization are performed on the Yulingling ointment through the three key components, so that favorable support is provided for industrial production of the Yulingling ointment.

This application discloses an intramolecular hydrogen bond-enhanced anthraquinone derivative, its synthesis method, and its application, relating to the field of electrochemical energy storage. The acetic acid-modified anthraquinone RH-DA or AE-DA is 2,2'-(3-carboxyl-1,8-dihydroxy-9,10-dioxo-9,10-dihydroanthracene-2,7-diyl)diacetic acid and 2,2'-(1,8-dihydroxy-3-(hydroxymethyl)-9,10-dioxo-9,10-dihydroanthracene-2,7-diyl)diacetic acid. Rhein or aloe-emodin is dissolved in NaOH, glyoxylic acid solution and sodium dithionite are added, and after stirring, sodium dithionite is added again. After stirring and cooling to room temperature, hydrogen peroxide solution and HCl solution are added, causing precipitation. After washing with HCl, the precipitate is dried in a vacuum drying oven to obtain a black-green solid RH-DA or a yellow-green solid AE-DA. Its application in alkaline aqueous organic flow batteries is also disclosed.

The application discloses a method for establishing HPLC fingerprint spectrum of Lianhuaqingwen capsules based on chemical identification method, and is characterized in that the method adopts high performance liquid chromatography and specifically comprises the following steps: 1) preparation of reference solution: precisely take new chlorogenic acid, quercitrin, forsythia glycoside, amygdalin, emodin, chrysophanol, chlorogenic acid, forsythia glycoside A, rhein, glycyrrhizic acid reference, and add methanol to prepare a reference solution; 2) preparation of test sample solution: 3) chromatographic conditions: the mobile phase is 0.1% phosphoric acid aqueous solution (A)-acetonitrile (B), gradient elution; the detection wavelength is 207 nm, the column temperature is 30 DEG C, and the flow rate is 1.0 mL·min ‑1 ; 4) determination: precisely take the test sample solution and inject it into a high performance liquid chromatograph, and the HPLC fingerprint spectrum is obtained by HPLC determination. The HPLC fingerprint spectrum established by the application is helpful for further improving the quality evaluation system of Lianhuaqingwen capsules and can provide a reference for the quality control of Lianhuaqingwen capsules.

A fructus cannabis intestine-moistening pill high performance liquid chromatography fingerprint establishment method relates to the field of medicine, and comprises the following steps: weighing fructus cannabis intestine-moistening pill medicinal material powder, adding methanol, plugging, weighing, soaking, carrying out ultrasonic extraction, cooling, weighing, complementing the weight loss, uniformly shaking, and filtering to obtain a test solution; the method comprises the following steps: weighing an emodin reference substance, a rhein reference substance, a chrysophanol reference substance, a hesperidin reference substance, a costunolide reference substance, a dehydrocostunolide reference substance and a paeoniflorin reference substance, adding methanol to prepare a mixed reference substance solution, and filtering to obtain a reference substance solution; sucking the test sample solution and the reference substance solution, injecting the test sample solution and the reference substance solution into a high performance liquid chromatograph, measuring and recording a chromatogram, importing detection results of the test sample solution and the reference substance solution into fingerprint spectrum software, and generating a reference fingerprint spectrum through data matching. The quality of the fructus cannabis intestine moistening pill can be effectively represented, the quality of the medicine can be comprehensively monitored, and the method has the advantages of being high in stability, high in precision and good in reproducibility.

The present invention discloses a pharmaceutical composition for treating gastrointestinal motility disorders and a preparation method thereof, belonging to the field of medical technology. The active ingredients of the pharmaceutical composition are rhein and glycyrrhizic acid, with the mass ratio of rhein to glycyrrhizic acid being 1:20. The present invention is beneficial in that research has found that combining rhein and glycyrrhizic acid can significantly improve gastrointestinal motility, with the most effective effect being achieved when the mass ratio of rhein and glycyrrhizic acid is 1:20.

The present application belongs to the technical field of industrial analysis and detection, and provides a chrysophanol-based natural perfume reconstituted molecular tool, a preparation method and application thereof. The method comprises the following steps: mixing a natural perfume solution and a chrysophanol solution to perform a dehydration condensation reaction, and obtaining the chrysophanol-based natural perfume reconstituted molecular tool. The raw material is abundant and belongs to a natural plant extract, the cost is low, the reagent required in the whole process is single, is non-toxic and harmless, and the final yield is also high. The obtained molecular tool has a stable chemical structure, can exist in a complex wax emulsion for a long time, has stable light signal release performance, can effectively release a light signal in a wax emulsion with various pH values, can maintain good light signal output in a long-term irradiation process, has a high viscosity sensitivity coefficient, and has a detection lower limit of viscosity as low as 1.23 cP, is suitable for measuring wax emulsion micro-area viscosity changes, and is helpful to realize rapid, efficient and visual preparation of wax emulsion consistency.

The present application relates to the technical field of medical polymer materials, and particularly relates to a drug mineralized microstick, a preparation method and application thereof. The preparation method of the drug mineralized microstick provided by the present application comprises the following steps: A) mixing rhubarb acid and alkali liquor to obtain a first solution; B) mixing the first solution with a degradable high polymer compound to obtain a second solution; the degradable high polymer compound contains carboxylate; C) mixing the second solution with a metal salt solution to obtain a third solution; D) incubating the third solution under constant temperature and humidity to obtain the drug mineralized microstick. The drug mineralized microstick prepared by the present application has a highly crystalline structure and the ability to effectively release small molecule drugs; the drug mineralized microstick has the characteristics of low hemolysis and low cytotoxicity; in an environment of excessive inflammatory response, the drug mineralized microstick can effectively reduce the expression of proinflammatory mediators, and exhibits high anti-inflammatory activity.

The present application belongs to the field of medicine, and particularly relates to a quality detection method of a Shengqing Jiangsuan mixture. The present application firstly establishes a characteristic spectrum method for simultaneously detecting 10 main active components (paeoniflorin, puerarin, gentiopicroside, loganin acid, astilbin, benzoylpaeoniflorin, resveratrol, rhein, pachymaran A and pachymaran B) in the mixture, overcomes the limitation of single component control quality, and can more comprehensively and objectively evaluate the overall quality of the medicine. Through optimizing the sample pretreatment process and the chromatographic gradient elution conditions, the present application effectively eliminates the interference of the complex matrix, and ensures the good separation of each component. The method has strong specificity, no interference of negative, and can accurately identify the target component.