The present invention provides an information system and a method of public security criminal technical inspection and identification. The method has the following procedures: authorizing identification, accepting the authorized identification, delegation of inspection of cases, implementing identification, professional verification, administrative examination and approval, returning the cases, picking up documents, keeping material evidences, filing the documents, integrated modification, integrated inquiry, integrated statistics, working out statistical forms, personnel management, managing the assets, attendance management, network office operation, and system management. The system comprises three levels, wherein, a first level is application, comprising application of dual-server clustering and WEB server dual-clustering; a second level is database which takes two highly functional servers as the database clustering servers and uses independent exchanger equipment; a third level is data storage, adopting an EMC high-end array. The present invention can meet the needs of the criminal technical inspection and identification for a public security system.