56results about How to "Quantitative realization" patented technology

The invention relates to a nested quantitative PCR detection method for virus covalently closed circular DNA (ccc DNA) of hepatitis b virus. The particular detection steps are: 1) extraction DNA of hepatitis b virus; 2) design and synthesis primers and probe: design an internal and an external primer at conservative region of both sides of negative chain gap of HVB DNA, with Taqman fluorescence probe arranged at the lower part of the negative chain gap; 3) Plasmid-SafeTM ATP-Dependent DNase restriction enzyme purification; 4) nested real time quantitative PCR amplification, comprising: common PCR-template with external primer amplification purification and real time quantitative PCR-fluorescence quantitative amplification to common PCR production with internal primer and fluorescence probe. The invention can improve the specificity and sensitivity of detection reactive to make the operation process and result analysis much more accurate, simple and time saving.

The invention provides a quantitative feeding device for chicken feed and belongs to the technical field of livestock quantitative feeding equipment. The quantitative feeding device for chicken feed has the beneficial effects of realizing quantitative feeding in the whole process and automatic control for accurate timing and guaranteeing sufficient feed supplied to livestock. Besides, a rotary blanking cabin can rotate around a spindle, so that feed therein can accumulate above a blanking pipe under the effect of centrifugal force, the blanking is convenient and the feed is prevented from rotting due to long-term accumulation away from the blanking opening of the rotary blanking cabin.

The invention discloses a method for establishing a quantitative model of auxiliary materials of Chinese medicinal granules. The method comprises the following steps: (1) acquiring infrared spectra of the Chinese medicinal granules of auxiliary materials with different proportions, and establishing different model samples so as to form a model database; (2) comparing parameters of the model samples at different sensitive wave number bands, and screening quantitative wave number bands; (3) verifying the wave number section models with relatively excellent parameters, and enabling the wave number bands of the verified result to be corresponding parameters of the quantitative models; and (4) predicting the content of the auxiliary materials of a plurality of Chinese medicinal granules by the quantitative models, and comparing the predicted result with the content of known auxiliary materials so as to determine the parameters of the final quantitative model. The method is used for quantifying the auxiliary materials in the Chinese medicinal granules by utilizing an infrared technique, and the established auxiliary quantitative model can simply and rapidly determine the auxiliary material adding amount in the Chinese medicinal granules, and an important means is provided for evaluating the quality of the Chinese medicinal granules.

The invention relates to an online analyzer for excess of caustic soda. The online analyzer comprises a tank body, a control system and a detection system are arranged in the tank body, and the control system is connected with and used for controlling the detection system, The analyzer is characterized in that the detection system comprises a reaction cup, a trace charge pump, a PH gauge, a quantitative system, a feed system and a discharge system, wherein the output end of the feed system, the output end of the trace charge pump, the input end of the discharge system, the input end of the quantitative system and the PH gauge extend into the reaction cup.

A rapid detection method of tobacco leaf volatile components is characterized in that the method adopts dichloromethane for extraction of tobacco leaves, a sample after extraction is separated by a chromatographic column, a tail end shunt of the chromatographic column is regulated by using a micro plate flow path control technology, at the same time, the shunt enters a quadrupole time-of-flight mass spectrometer (QTOF MS) and a flame ionization detector (FID) and is determined, accurate qualitation and quantitation of the tobacco leaf volatile components is achieved, the whole process has the advantages of simple operation and short use time, and mass and automatic processing of the sample is achieved. The process can be completed by only requiring a simple solvent for extraction, a small amount of an organic solvent is consumed, the whole process has no second-order reaction problem of the tobacco leaf volatile components, and actual conditions of the sample is really reflected; an extract liquid is detected by using gas chromatography-quadrupole time-of-flight mass spectrometry/flame ionization detection for a first time, only one-needle sample injection is needed, and a QTOF MS spectrogram and an FID spectrogram are obtained, wherein QTOF MS can perform accurate quantitation of components of complex samples while the FID can perform accurate quantification of samples with wide concentration scope, and the retaining time of the QTOF MS spectrogram and the retaining time of the FID spectrogram are just the same.

The invention relates to a quick oil grease oxidation degree detection method, in particular to a quick oil grease oxidation degree test box manufactured based on the quick oil grease oxidation degree detection method. According to the quick oil grease oxidation degree detection method, based on a Kreis test, a color reaction between an oil grease oxidation secondary decomposition product and phloroglucinol is conducted; by adjusting the addition proportion of an acid solution and a phloroglucinol solution, the reaction speed can be increased, color developing stability can be improved greatly, and the instability defect of an original experiment is overcome; the layering velocity is increased greatly, the clarified and transparent colorimetric layer is obtained quickly, and the interference of contrasting colors of oil samples is reduced; furthermore, the concentration of the used acid solution can be lowered greatly, operation is safer, and pollution to the environment by waste is reduced.

The invention provides an ultrafast PCR-based colorimetric sensing new method used for visible ultrasensitive detection of Hg<2+>. According to the method, an amplification primer and a template of anultrafast Polymerase Chain Reaction (PCR) are designed according to mercury ion thymine mismatch, a color development module is self-assembled through the primer by combining enzyme-linked nucleic acid, so that a new mismatch-based mercury ion target function nucleic acid detection method and a biological sensor are created. The detection method and the biological sensor are faster and more sensitive than traditional methods, have characteristics of strong specificity, high sensitivity, and reliable detection result, simplify analysis and detection steps, shorten analysis time, moreover, makeonline real-time detection possible, and are portable and suitable for field work, thereby having a bright application prospect in the field of heavy metal rapid detection.

The invention belongs to the field of biotechnology, particularly relates to the field of virus detection, and more particularly relates to a PCR primer group, probe and kit and detection method for detecting human immunodeficiency virus-1 2-LTR DNA. The detection primer group provided by the invention comprises nucleotide sequences shown as SEQ ID NO: 1-6; and the detection probe comprises nucleotide sequences shown as SEQ ID NO: 7-8. The detection primer group provided by the invention is different from the conventional real-time fluorescence quantitative PCR; through introduction of 3 pairs of real-time fluorescent quantitative PCR primers, that is, 6 primers, adoption of multiple cycles, and increase of the primer annealing temperature, the PCR detection method for detecting the HIV-1 2-LTR DNA, provided by the invention, achieves extremely high sensitivity and specificity, and the minimum detection limit can reach 2 copies per million cells in each PCR; and moreover, when a quantitative cell reaction system is further added in the PCR reaction, the HIV-1 2-LTR DNA and the number of cells can be quantified at the same time in the same PCR reaction (in the conventional method, the cell count is additionally required).

The invention discloses a quantitative bottle. The quantitative bottle comprises a bottle body sleeved with a bottle cover; a guide tube is inserted in the bottle cover, the upper end of the guide tube is located above the bottle cover, and a quantitative bin is arranged at the position, close to the bottom of an inner cavity of the bottle body, of the lower portion of the guide tube; the quantitative bin is provided with a liquid inlet communicating with the inner cavity of the bottle body; the liquid inlet is provided with a one-way valve for one-way communication from the inner cavity of the bottle body to the quantitative bin; and the bottle body at least has the elastic compression function. According to the quantitative bottle, when the bottle body is pressed, pressure can be formedfor liquid in the inner cavity of the bottle body and enables the liquid to overcome the action of the one-way valve and to enter the quantitative bin, the liquid in the quantitative bin can be directly poured out from the guide tube when reaching the preset amount, when the bottle body pours the liquid in the quantitative bin out, the liquid in the inner cavity of the bottle body cannot enter thequantitative bin as the liquid inlet is sealed by the one-way valve, and thus quantification of the liquid is achieved; and the quantitative bottle can be applied to the field of agent bottles, soy sauce bottles and the like with a quantitative usage requirement.

The invention provides a wastewater treatment device capable of realizing quantitative feeding of medicinal liquid, which includes a stirring tank, wherein the stirring tank is provided with a stirring chamber with the top opened, a plurality of medicinal liquid adding cylinders are circumferentially arranged in the stirring chamber, a medicinal liquid outlet is arranged at the bottom of each medicinal liquid adding cylinder, a quantitative medicinal liquid discharging mechanism includes a fixed ring, a toothed ring, a plurality of sealing sheets, a gear and a first driving mechanism, the central axis of the fixed ring coincides with those of the medicinal liquid outlets, the toothed ring is arranged at the outer part of the fixed ring and the central axis of the toothed ring coincides with that of the fixed ring, the plurality of sealing sheets are in circumferential uniform distribution with the center of the fixed ring as a center, one end of each sealing sheet is articulated with the fixed ring, the toothed ring is circumferentially articulated with a plurality of connecting rods, each connecting rod is articulated with one end of the corresponding sealing sheet, the gear is arranged outside the toothed ring and is engaged with the toothed ring, and the first driving mechanism is connected with the gear to drive the gear to rotate. The quantitative feeding of the medicinalliquid is achieved by setting the medicinal liquid adding cylinders and the quantitative medicinal liquid discharging mechanism, so as to achieve the best effect of wastewater treatment.

The invention discloses a trace liquid quantifying device, a sampling and sample adding method and application in nucleic acid testing, the trace liquid quantifying device comprises a thin rod, one end of the thin rod is used as a sampling end, the trace liquid quantifying device adopts a disposable thin rod to dip a sample and add a sample, and the invention relates to the technical field of trace liquid quantitative sampling. According to the trace liquid quantifying device, the sampling and sample adding method and the application in the nucleic acid testing, sampling and sample adding aredesigned on the basis of the principle that liquid is adsorbed on the surface of an object, rapid, simple and convenient quantitative sampling and sample adding are achieved, the problem of quantitative sampling and sample adding of trace liquid is solved, and template taking and adding operation of a nucleic acid testing is quite easy; the sample taking and adding method is particularly suitablefor sample adding of a trace sample template such as a nucleic acid testing, sampling and sample adding are achieved by adsorbing liquid through the surface of an object, the structure is simple, operation is very easy and convenient, and the method is very suitable for non-professionals to use on site.

The invention discloses a disc-type microfluidic fluorescence immunoassay analyzer and an immune item test method, and solves the technical problem that a detection instrument in the prior art cannotbe matched with a disc-type microfluidic fluorescence immunoassay detection chip for multi-item joint inspection by one-time sample adding to perform immune item detection. The immunoassay analyzer mainly comprises an analyzer main body and an analyzer movement. The immune item test method mainly comprises the following steps: the immune detection chip is put into the analyzer core, and differentspeeds are selected through the BLDC brushless motor; through centrifugation and capillary action, reagents and samples in the chip are uniformly mixed, transferred and reacted, and LED signal flickering and photoelectric conversion in the light path detection module are matched to complete detection. The analyzer is simple in structure, scientific and reasonable in design and convenient to use, can be accurately matched with an immunoassay chip so as to enable a reagent sample in the immunoassay chip to react and perform immunoassay item detection on the reacted reagent sample, and is high indetection efficiency and detection accuracy.

The invention belongs to the technical field of synthesis of a gas-sensitive film and more particularly relates to a parallel synthesizer and a synthesis method for the gas-sensitive film based on micro-droplet premixing and transfer-printing. The parallel synthesizer for the gas-sensitive film comprises a limiting objective table, a control module, a three-dimensional sliding table, a micro-droplet premixing module and a micro-droplet transfer-printing module; the micro-droplet premixing module can realize premixing of gas-sensitive raw materials by combination of an array peristaltic pump and an array micro-droplet syringe needle; and the micro-droplet transfer-printing module can realize quantitative micro-dropping and precise positioning and shaping of the gas-sensitive film in a transfer-printing process by co-coordination of an airtight automatic micro-syringe, an image positioning camera and a substrate of a prefabricated positioning film. The invention also discloses a preparation method of the gas-sensitive film. The parallel synthesizer provided by the invention can realize quantitative and uniform mixing of gas-sensitive materials which are different in materials and components, can be used for precisely and rapidly preparing various types of gas-sensitive films, can also prevent the problem of blocking in a printing process, and is applicable to nL-level quantitative micro-droplet premixing and transfer-printing of a nanometer gas-sensitive raw material suspension of which the size is not larger than 1 micron.

The invention relates to a grease oxidation degree fast detection method and a grease oxidation degree fast detection testing box prepared on the basis. The method is based on a Kreis test, namely the color development reaction of grease oxidation secondary decomposed products and phloroglucinol. By adjusting the ratio of an added acid solution to an added phloroglucinol solution, reaction speed can be greatly increased, color development stability can be greatly improved, and the defect that an existing test is not stable is overcome. The layering speed is greatly increased, a clarified and transparent colorimetric layer can be fast obtained, and interference of oil sample contrast color is reduced. Besides, the concentration of the acid solution in use can be greatly reduced, operation is safer, and pollution of waste to the environment is reduced.

The invention relates to the technical field of firework internal cylinder charging equipment, in particular to a quantitative filling device for a powder material. The quantitative filling device forthe powder material comprises a feed hopper, a filling rack, a bin assembly and a quantitative assembly; the bin assembly is fixed to the middle of the filling rack, and the bottom of the bin assembly is provided with the quantitative assembly; and the feed hopper is arranged above the bin assembly, and the feed hopper is fixed to the filling rack, and communicates with the bin assembly through afeeding pipe. According to the quantitative filling device, quantitative, safe and efficient filling of filling materials in firecracker cakes is achieved, and the filling efficiency of the firecracker cakes is improved; and the quantitative filling device is suitable for further popularization and application.

The invention provides a novel IC card automatic loading and delivery system and method. The system includes a weighbridge weighing system, a loading system, an IC card, two card readers, two upper computers and an ERP server; the first upper Weighing management software and ERP system are installed on the first computer, and the loading management software and ERP system are installed on the second upper computer. The upper computer reads and writes the IC card through the card reader; both upper computers communicate with the ERP server . Real-time order delivery according to the ERP system, avoiding misdelivery when the customer has no order or the order has not yet taken effect; the difference between the delivery volume detected by the flow meter and the two weighing data is compared and verified to avoid cheating; the operator swipes the IC card for verification , to avoid misoperation, and record in the ERP system to improve the level of control; to avoid common problems in the loading process such as wrong delivery, multiple delivery, less delivery, cheating, and random operation.

The invention provides a quantitative feeding device, feeding equipment and 3D (3-Dimensional) printing equipment, relates to the technical field of 3D printing and aims to solve the technical problems, in the prior art, that aids of a mixed 3D printing material are tedious to weigh and differences of addition consistence can be caused because of a manual addition mode. The invention relates to the quantitative feeding device which comprises an auxiliary material hopper unit, wherein the auxiliary material hopper unit comprises a storage cabin and a quantitative discharge component; the storage cabin comprises a cabin body and a cabin bottom; the cabin bottom is an inclined plane of which one end is higher than the other end; the quantitative discharge component comprises a rotating cabin;partitioning grids are arranged opposite to a first discharge; a second opening is formed in the rotating cabin. The invention further relates to the feeding equipment which comprises the quantitative discharge component. The invention further relates to the 3D printing equipment which comprises the feeding equipment. By adopting the quantitative feeding device, the feeding equipment and the 3D printing equipment, quantitative feeding of materials at regular time can be achieved, and artificial interference can be reduced.

The invention discloses an IoT (Internet of Things)-based superior agricultural water-saving irrigation system, and relates to the technical field of irrigation systems. The system comprises an irrigation assembly, a plurality of soil humidity sensors, a water supply device and a control device, wherein the water supply device comprises a quantitative box, a water storage pool, a first water pumpand a second water pump, a first water pipe is fixed at a water outlet of the first water pump, one end of the first water pipe is located in the quantitative box, a transverse plate is fixed at the upper part of one side of the quantitative box, and a liquid level sensor is fixed on one surface of the transverse plate. According to the system, water can be extracted into the quantitative box through the first water pump and the first water pipe, water height in the quantitative box can be monitored by the liquid level sensor, timing can be performed by a timer, a second switching valve can becontrolled to be closed and opened and timing and quantitative processing can be performed on irrigation by a processor, and the problem that the water consumption of an existing irrigation system cannot be effectively controlled is solved.

The invention discloses a molecular detection method for rapid quantification of diatom cell density. The method comprises the following steps: 1, filtering a water sample to be tested by using a membrane, then extracting genomic DNA of the sample, and purifying; 2, carrying out fluorescence quantitative polymerase chain reaction (PCR) amplification on a rbcL gene of diatom by using a specific primer, and recording a Ct value; 3, taking the single cultured or pure cultured diatom with known cell density as a standard algae strain, extracting genomic DNA, carrying out gradient dilution and thencarrying out quantitative PCR amplification by using the diluted product as a standard template, and drawing a fluorescence quantitative PCR standard curve according to the Ct value; 4, calculating the copy number of the sample rbcL gene according to the Ct value and the standard curve, wherein the cell density of the diatom in the sample is equal to the copy number. The method is simple, convenient and easy to operate, has very high sensitivity and accuracy, can be used for rapidly and accurately quantifying the cell density of the diatom in the water sample, and is suitable for the specificquantitative detection of diatoms.

The quantitative feeding valve for casting comprises a valve shell and a valve element arranged in the valve shell, the valve shell comprises a feeding port and a discharging port, the valve element comprises an inner cavity and a quantitative shaft rotationally arranged in the inner cavity, the valve element is cylindrical, quantitative grooves are evenly distributed in the valve element in the circumferential direction of the valve element, and the quantitative grooves are communicated with the inner cavity. Quantitative blocks are arranged in the quantitative grooves and connected into the quantitative grooves in a sliding mode, the volume of the quantitative grooves is adjusted by adjusting the positions of the quantitative blocks in the quantitative grooves, a buffer cavity is formed between the valve shell and the valve element, media discharged by the quantitative grooves enter the buffer cavity, and the discharging port is communicated with the buffer cavity. The quantitative grooves located in the valve element are communicated with the discharging port one by one in the rotating process of the valve element. According to the scheme, in the actual application process, the quantifying grooves can effectively quantify media, and then the valve element has the reasonable rotating speed, so that the flow speed of the media and the adding amount of the media can be effectively controlled.

According to a screening method based on superfast PCR (Polymerase Chain Reaction) and GICA (Gold Immunochromatographic Assay) test paper, and a biosensor, established by the invention, the overall screening time of transgenic crops is controlled in about 10 minutes; compared with a traditional method, the screening method is quicker and more flexible and has the advantages of strong specificity,high sensitivity, reliable detection results and the like, analysis and detection steps can be simplified, the analysis time can be shortened, more importantly, online in-time detection becomes possible, carrying and field operation are convenient, and the screening method has a very good application prospect in the field of rapid screening of the transgenic crops.

The invention provides a raw material measuring device and a preparation blending device and a preparation blending system. The raw material measuring device comprises a moving module, a fixing moduleand a driving module, wherein an injector is mounted between the moving module and the fixing module; and the driving module is used for driving the moving module to move towards the fixing module, and then the injector can be compressed to extrude out a liquid raw material from the injector. The preparation blending device comprises at least two raw material measuring devices, an information receiving module, a controller and a base; the information receiving module is used for receiving preparation formula information and used for transmitting the formula information to the controller; andthe controller is used for controlling the raw material measuring device to measure liquid raw materials. Compared with a conventional mode that liquid raw materials are sucked and blended one by oneby medical operators, the preparation blending device is relatively simple in blending process, the blending time can be shortened, the liquid raw material measuring precision can be improved, and inaddition, the liquid raw materials can be easily uniformly stirred since multiple liquid raw materials are measured simultaneously.

The invention relates to an electronically-controlled automatic shaping mechanism for ceramic wall and floor tile patterns, which relates to feeding and distributing device for producing ceramics made of imitated natural stones and various textures. The electronically-controlled automatic shaping mechanism comprises a frame and a transmission belt, a group or a plurality of groups of feed assemblies are arranged on the frame, each feed assembly comprises a group or a plurality of groups of feed mechanisms, a plurality of crushing mechanisms, a mixing and smashing mechanism and a distributing mechanism, each distributing mechanism refers to a distributing port with two guide bevel edges, the crushing mechanisms and the mixing and smashing mechanisms are arranged among the corresponding feed mechanisms and the corresponding distributing mechanisms, the crushing mechanisms are arranged below the corresponding feed mechanisms, the mixing and smashing mechanisms are positioned among the corresponding crushing mechanisms and the corresponding distributing mechanisms, the transmission belt is arranged below the distributing mechanisms, and the feed assemblies and a control system of the transmission belt are connected to a computer control operation system. The electronically-controlled automatic shaping mechanism for the ceramic wall and floor tile patterns is high in distributing precision, so that the distributed patterns are diversified, and manual operation is greatly reduced.