Screening method for human gastric cancer drug-resistant cell specific combination and drug-resistant reverse short peptide and sequence

A drug-resistant and specific combination technology for gastric cancer, applied in the field of biomedicine

Inactive Publication Date: 2008-03-26
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

However, the 200510042810.4 patent only simply screens for polypeptides that specifically bind to cells, and does not specifically screen for polypeptides that affect cell functions

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  • Screening method for human gastric cancer drug-resistant cell specific combination and drug-resistant reverse short peptide and sequence
  • Screening method for human gastric cancer drug-resistant cell specific combination and drug-resistant reverse short peptide and sequence
  • Screening method for human gastric cancer drug-resistant cell specific combination and drug-resistant reverse short peptide and sequence

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Embodiment Construction

[0145] Gastric cancer drug-resistant cell surface molecular markers are one of the key targets for gastric cancer multidrug resistance reversal therapy. Through in vitro whole cell screening of phage random peptide library, combined with MTT in vitro drug susceptibility test function identification, a group of human gastric cancer drug resistance has been successfully obtained Cell specific binding and reversal of drug-resistant short peptide GMBPs, and using phage cell binding experiment, immune cell / histochemical staining, cell binding competition inhibition experiment, immunofluorescence detection and other methods to identify the binding specificity of GMBPs, and further using polypeptide MTT In vitro drug susceptibility experiments and in vivo drug susceptibility experiments in nude mice confirmed the reversal of drug resistance characteristics of GMBPs. The specific methods are as follows:

[0146] 1. Technical solution

[0147] 1.1. In vitro whole cell subtractive screening...

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Abstract

The present invention belongs to the field of biomedicine technology, and is screening method and sequence for human gastric cancer drug resistant cell specific combination and drug resistant reverse short peptide. The present invention features that through the combined random bacteriophage peptide library and MTT extracorporeal drug sensitive test screening, bacteriophage cloning polypeptide capable of reversing the drug resisting characteristic of the drug resistant gastric cancer cell is obtained to lower the survival rate and IC50 value of drug resistant gastric cancer cell under chemotherapy medicine and result in statistic difference cloning (p<0.05). Bacteriophage polypeptide is utilized in screening whole cell and the ligand of specific expression molecule on drug resisting tumor cell membrane, so as to the bioactive polypeptide and corresponding bioactive short peptide and its sequence (GMBPs).

Description

Technical field [0001] The invention belongs to the field of biomedicine technology, and specifically relates to a screening method and sequence of short peptides for specific binding and drug resistance reversal of human gastric cancer drug-resistant cells. Background technique [0002] Gastric cancer is the malignant tumor that causes the largest number of deaths in my country. Chemotherapy is one of the main methods for the treatment of gastric cancer, but its therapeutic effect has not made significant progress. The main reason is that gastric cancer cells develop multidrug resistance to chemotherapeutic drugs. Multi-drug resistance (MDR) of tumor cells means that after tumor cells are resistant to a certain chemotherapeutic drug, they also develop cross-resistance to other chemotherapeutic drugs with different chemical structures and mechanisms. [0003] Current studies have shown that the production of multidrug resistance in tumor cells is mainly related to the following m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G01N33/68G01N33/574
Inventor 林涛丁杰梁树辉韩全利吴开春樊代明
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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