Application of isosteviol lactone in preparation of tumor drug sensitizers

A technology of isosteviol lactone and antitumor drug, which is applied in the application field of isosteviol lactone in the preparation of tumor drug sensitizers, can solve the problem that isosteviol lactone has not been reported, and achieves overcoming drug resistance. Sex, inhibit proliferation, enhance the effect of treatment

Inactive Publication Date: 2015-03-25
FUDAN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] So far, there has been no report on the regulation of isosteviol lactones on the drug resistance of tumor cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of isosteviol lactone in preparation of tumor drug sensitizers
  • Application of isosteviol lactone in preparation of tumor drug sensitizers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Implementation 1: CCK-8 kit detects the multiple resistance of drug-resistant cell lines to isosteviol lactones

[0045] Experimental Materials:

[0046] Isosteviol lactone was purchased from the Institute of Materia Medica, Chinese Academy of Sciences, with a purity of not less than 98%. Human oral cancer cell line KB and its drug-resistant cell line KB / VCR were provided by the Chinese Academy of Sciences, human breast cancer cell line MCF-7 / ADR and its drug-resistant cell line MCF-7 / ADR, human colon cancer HCT-8 and The drug-resistant cell line HCT-8 / VCR was purchased from Nanjing Kaiji Biological Company. Verapamil (VPL), vincristine (VCR) and doxorubicin (ADR) were purchased from Roche Chemical Company with a purity greater than 99%. CCK-8 kit was purchased from Tongren Company.

[0047] experimental method:

[0048] Cell recovery

[0049] 1) Take out the cryotube from the liquid nitrogen tank, put it directly into warm water at 37°C, and shake it from time to ...

Embodiment 2

[0070] Example 2: CCK-8 method detects the reversal effect of isosteviol lactones on the multidrug resistance activity of tumor cells

[0071] Experimental material: with embodiment 1.

[0072] experimental method:

[0073] Isosteviol lactone sensitization experiment: KB / VCR, MCF-7 / ADR and HCT-8 / VCR cells were inoculated into 96-well plates at a density of 3500 / well, 24 hours later with different concentrations of VCR, and different concentrations of VCR It was formulated with isosteviol lactone in α-MEM containing 10% fetal calf serum and added to each well. After cultivating for 48 hours, discard the culture medium, and use the CCK-8 kit to measure the activity of the drug-resistant cell line and its parental cells to VCR and VCR + isosteviol lactone with the method in Example 1, and draw a curve. Three replicate wells were set up for each concentration, and the experiment was repeated three times.

[0074] The experimental results showed that the multiples of drug resist...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of drugs, relates to an application of isosteviol lactone in the preparation of tumor drug sensitizers, and especially relates to an application of isosteviol lactone in the preparation of antitumor drug resistance-reversal agents. The isosteviol lactone is a natural product, has a tumor cell multidrug resistance reversal effect, can be used as a tumor multidrug resistance reversal agent, can increase the effect of tumor multidrug resistance cells on the antitumor drug sensitivity, and can be used as a chemotherapy sensitizer. The invention also provides a method using a medicinal composition containing an antitumor drug and the isosteviol lactone to inhibit the proliferation of tumor multidrug resistance cells. The micromolecular compound isosteviol lactone can be developed as a new antitumor drug or its auxiliary component, has an obvious antitumor effect, is green and environmentally-friendly, and provides a new approach for the treatment and curing of tumors.

Description

technical field [0001] The invention belongs to the pharmaceutical field of reversing tumor multidrug resistance and sensitizing antitumor drugs, and specifically relates to the new application of isosteviol lactones, that is, isosteviol lactones are used in the preparation of tumor multidrug reversal agents and antitumor drug sensitization application in agents. Background technique [0002] Malignant tumors seriously endanger human life and health, and have become one of the main causes of death from diseases today. At present, the main treatment methods for malignant tumors include surgery, radiotherapy and chemotherapy. Chemotherapy is a better option for patients diagnosed with advanced tumors and those with metastases. Clinicians can optimize the maximum curative effect and minimum toxicity by adjusting the dosage of drugs, helping patients prolong survival time and improve the prognosis of life quality. However, the generation of drug resistance of tumor cells lead...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/366A61P35/00A61K31/475A61K31/704A61K31/337A61K31/513
Inventor 余龙朱恒锐
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap