A kind of tumor chemotherapeutic drug preparation combination

A chemotherapeutic drug and a chemotherapeutic drug technology, which is applied in the field of combination of tumor chemotherapeutic drug preparations, can solve the problems of undeveloped and other problems, and achieve the effect of reversing the drug resistance of tumor cells, simple operation, and enhancing the effect of killing tumor cells

Active Publication Date: 2019-09-10
HUBEI SOUNDNY BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, drugs as MDR reversal agents for clinical application have not been successfully developed so far.

Method used

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  • A kind of tumor chemotherapeutic drug preparation combination
  • A kind of tumor chemotherapeutic drug preparation combination
  • A kind of tumor chemotherapeutic drug preparation combination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation of cell vesicles

[0042] 1) Experimental materials and reagents

[0043] H22 mouse liver cancer cells were obtained commercially, the ultraviolet device was owned by a conventional biological safety cabinet, the transmission electron microscope was JEM1010 (JEOL, Japan), and the filter membrane brand was PALL (Part No: J100047050).

[0044] 2) Experimental steps

[0045] Cultivate H22 mouse liver cancer cells in RPMI-1640 cell culture medium to make the cell volume reach 2×10 7 / ml; H22 mouse liver cancer cells were irradiated with ultraviolet rays for 60 minutes;

[0046] Eighteen hours after ultraviolet irradiation, the H22 mouse liver cancer cell apoptosis was observed under a microscope. The cell vesicles of the above apoptotic tumor cells were collected by centrifugation, including: centrifuging at 1300rpm and 5000rpm for 10 minutes respectively, and then centrifuging at 14000g for 1 minute to remove cells and debris. The centrifugal forc...

Embodiment 2

[0049] Example 2 The effect of the combination of tumor chemotherapy drug preparations of the present invention on the efflux of doxorubicin by human breast cancer cells.

[0050] 1) Experimental materials and reagents

[0051] The human breast cancer cell line MCF-7, the drug of doxorubicin hydrochloride was purchased commercially, the ultraviolet device was owned by a conventional biological safety cabinet, and a two-photon confocal microscope was used.

[0052] 2) Experimental steps

[0053] Human breast cancer cell line MCF-7 was cultured in RPMI Medium1640 cell culture medium to make the cell volume reach 2×10 7 / ml; The human breast cancer cell line MCF-7 was irradiated with ultraviolet rays for 60 minutes; 18 hours after ultraviolet irradiation, the apoptosis of the human breast cancer cell line MCF-7 was observed under a microscope; the apoptotic tumor was collected by centrifugation or filtration Cell vesicles, the method is the same as in Example 1;

[0054] Norma...

Embodiment 3

[0058] Example 3 Effects of the combination of tumor chemotherapy drug preparations of the present invention on the uptake of doxorubicin hydrochloride by MCF-7, MCF-7 / TRC and ADR / MCF-7 cells

[0059] 1) Experimental materials and reagents

[0060] Human breast cancer cell line MCF-7, drug-resistant tumor regenerative cell MCF-7 / TRC (tumour-repopulating cancer cell), human breast cancer doxorubicin hydrochloride-resistant strain ADR / MCF-7, used for culture Three-dimensional fibrin glue, doxorubicin hydrochloride drug, lysosomal membrane protein lamp-2 antibody, FITC fluorescent secondary antibody, and nuclear dye DAPI are commercially available. The ultraviolet device is owned by a conventional biological safety cabinet, and a two-photon confocal microscope is used.

[0061] 2) Experimental steps

[0062] According to the method of Example 2, the cell vesicles derived from the human breast cancer cell line MCF-7 were obtained;

[0063] 5,000 MCF-7 human breast cancer cells w...

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Abstract

The present invention provides a tumor chemotherapeutic drug preparation combination, which includes a cell vesicle preparation derived from apoptotic tumor cells for sensitizing tumor cells, and a chemotherapeutic drug preparation for treating the tumor. The tumor chemotherapeutic drug preparation combination provided by the present invention uses the cell vesicle preparation derived from apoptotic tumor cells as a tumor cell sensitizer, combined with the chemotherapeutic drug preparation for treating the tumor, can make more chemotherapeutic drugs enter into the Tumor cells, so as to reverse the drug resistance characteristics of tumor cells to a certain extent, and enhance the effect of killing tumor cells.

Description

technical field [0001] The invention relates to a combination of tumor chemotherapy drug preparations, in particular to a combination of tumor chemotherapy drug preparations comprising cell vesicles for sensitizing tumor cells and chemotherapy drugs. Background technique [0002] At present, the most widely used clinically is to use chemical drugs to destroy tumor cells. However, while most chemotherapy drugs kill tumor cells, they also cause great damage to normal human cells and produce toxic and side effects, so that chemotherapy cannot be carried out smoothly, seriously affecting the The curative effect, and repeated use of chemotherapy drugs can make tumor cells, especially the part of tumor cells with cell stemness, develop strong drug resistance, and then make chemotherapy drugs lose their killing effect on tumor cells. Chemosensitizers that are sensitive to chemotherapeutic drugs can improve the curative effect of chemotherapeutic drugs to a greater extent, reduce to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61K35/13A61P35/00A61K33/243
CPCA61K47/46A61K31/4745A61K31/513A61K31/519A61K31/704A61K35/13A61K45/06A61P35/00A61K33/243A61K2300/00A61K33/24
Inventor 黄波张一马婧薇
Owner HUBEI SOUNDNY BIOLOGICAL TECH
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