Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel cell and its use for preparing gastrodin

A technology of gastrodin and its use, which is applied in the field of new cells and its use in the preparation of gastrodin, and can solve the problems of easy pollution of the environment, high production cost, and poor economic benefits

Active Publication Date: 2009-07-22
KPC PHARM INC
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing synthetic gastrodin process has defects such as high preparation cost, flammable raw materials, easily polluted environment and poor economic benefits.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1. The preparation of gastrodin

[0016] Add 100ml of LS liquid medium and 15g of cells (strain) (CGMCC No.1122) into a 250ml Erlenmeyer flask, then add 70.00mg of p-Hydroxybenzaldehyde and culture in a shaker at room temperature for 12 days with a rotation speed of 100-120 rpm. The conversion solution was extracted with n-butanol, then the n-butanol extract was concentrated, and the concentrated residue was chromatographed on a silica gel column, eluted with chloroform:methanol (9:1, v / v), concentrated and eluted liquid to obtain crude gastrodin, which was recrystallized from chloroform:methanol (9:1, v / v) to obtain 56.66 mg of gastrodin with a yield of 80.95% and a melting point of 154-156°C.

Embodiment 2

[0017] Embodiment 2. The preparation of gastrodin

[0018] Add 100ml LS liquid medium and 15g cells (strains) (CGMCC No.1122) to a 250ml Erlenmeyer flask, rotate in a shaker at room temperature for 4-5 days at a speed of 100-120 rpm, and then add 30.00mg of Hydroxybenzaldehyde was cultured on a shaker at room temperature for 10 days with a rotating speed of 100-120 rpm. The conversion solution was extracted with n-butanol, then the n-butanol extract was concentrated, and the concentrated residue was chromatographed on a silica gel column, eluted with chloroform:methanol (9:1, v / v), concentrated and eluted liquid to obtain crude gastrodin, which was recrystallized from chloroform:methanol (9:1, v / v) to obtain 24.89 mg of gastrodin with a yield of 82.96% and a melting point of 154-156°C.

Embodiment 3

[0019] Embodiment 3. The preparation of gastrodin

[0020] Add 100ml LS liquid medium and 10g cells (strains) (CGMCC No.1123) to a 250ml Erlenmeyer flask, rotate in a shaker at room temperature for 4-5 days at a speed of 100-120 rpm, and then add 30.00mg of Hydroxybenzaldehyde was cultured on a shaker at room temperature for 10 days with a rotating speed of 100-120 rpm. The conversion solution was extracted with n-butanol, then the n-butanol extract was concentrated, and the concentrated residue was chromatographed on a silica gel column, eluted with chloroform:methanol (9:1, v / v), concentrated and eluted liquid to obtain crude gastrodin, which was recrystallized from chloroform:methanol (9:1, v / v) to obtain 25.80 mg of gastrodin with a yield of 86.00% and a melting point of 154-156°C.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a novel plant-derived cell, its use in preparing gastrodin and a method for preparing gastrodin. Further, the plant cells of the present invention are from the Solanaceae genus Datura, especially Datura alba and Datura violet.

Description

field of invention [0001] The present invention relates to a novel plant-derived cell, its use in preparing gastrodin and a method for preparing gastrodin. Further, the plant cells of the present invention are from the Solanaceae genus Datura, especially Datura alba and Datura violet. Background technique [0002] Gastrodin (p-hydroxymethylbenzene-β-O-glucopyranoside) is the main active ingredient of Gastrodia elata, which has sedative, anti-inflammatory, anti-convulsant, analgesic and immune-enhancing effects. Gastrodin has been prepared by chemical synthesis and the synthetic gastrodin has the same biological or pharmacological activity as gastrodin from natural sources. However, the existing gastrodin synthesis process has defects such as high preparation cost, flammable raw materials, easy pollution of the environment, and poor economic benefits. Therefore, it is necessary to develop a new economical, safe, pollution-free and efficient preparation method of gastrodin. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04C12P19/60
Inventor 龚加顺
Owner KPC PHARM INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com