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Method for preparing krev alcohol and use for preparing anti-acetylcholinesterase medicine

A technology of creviol and ethyl acetate, which is applied in the field of preparation of drugs for the treatment of Alzheimer's disease and other diseases related to acetylcholine deficiency, and achieves the effect of simple extraction and refining methods

Inactive Publication Date: 2010-01-20
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some studies have been carried out on the metabolites of endophytic fungi in mangrove plants, and many antibacterial and anti-tumor compounds with potential application prospects have been found, but the research and development of acetylcholinesterase inhibitors produced by such fungi is rare. to report

Method used

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  • Method for preparing krev alcohol and use for preparing anti-acetylcholinesterase medicine
  • Method for preparing krev alcohol and use for preparing anti-acetylcholinesterase medicine
  • Method for preparing krev alcohol and use for preparing anti-acetylcholinesterase medicine

Examples

Experimental program
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Effect test

Embodiment 1

[0037] A. Culture medium formula: slant medium formula: every liter of potato juice (peel the potato and cut it into small pieces, get 200g and put it into 1L 50% seawater, boil for 30min, filter, and use 50% seawater for the filtrate (seawater and tap water by volume) Add glucose 20g, agar 2.0g in ratio mixing) be settled to 1000ml). Seed culture medium formula: add 20g of glucose in every liter of potato juice (preparation method is the same as above). Fermentation medium formula: same as seed medium.

[0038] B. Fermentation process: slant culture: Prepare the culture medium according to the above slant culture medium formula, divide into test tubes, and prepare the slant after sterilizing at 121° C. for 30 minutes. The preserved endophytic fungus of mangrove plants, Aspergillus dwarfus CCTCC NO: M206063, was transferred to the slant medium and cultured at 25°C for 7 days. Shake flask culture: Prepare the medium according to the formula of the seed medium, pack into 500 E...

Embodiment 2

[0042] A. Medium formula: slant medium formula: per liter of potato juice (peel the potato and cut it into small pieces, take 300g and put it in 1L 100% seawater, boil for 30min, filter, and dilute the filtrate to 1000ml with 100% seawater) Add 30 g of glucose and 3.0 g of agar. Seed medium formula: add 30g of sucrose in every liter of potato juice (preparation method is the same as above). Fermentation medium formula: same as seed medium.

[0043] B. Fermentation process: slant culture: Prepare the culture medium according to the above slant culture medium formula, divide into test tubes, and prepare the slant after sterilizing at 121° C. for 30 minutes. The preserved endophytic fungus of mangrove plants, Aspergillus dwarfus CCTCC NO: M206063, was transferred to the slant medium and cultured at 28°C for 5 days. Shake flask culture: Prepare the medium according to the above seed medium formula, pack it into Erlenmeyer flasks (100ml / bottle), sterilize (121°C, 30min), insert t...

Embodiment 3

[0047] A. Culture medium formula: slant medium formula: every liter of potato juice (peel the potato and cut it into small pieces, get 150g and put it into 1L 20% seawater, boil for 30min, filter, use 20% seawater for the filtrate (seawater and tap water by volume) Add glucose 15g, agar 1.5g in ratio mixing) to 1000ml). Seed culture medium formula: add 15g of glucose in every liter of potato juice (preparation method is the same as above). Fermentation medium formula: same as seed medium.

[0048] B. Fermentation process: slant culture: Prepare the culture medium according to the above slant culture medium formula, divide into test tubes, and prepare the slant after sterilizing at 121° C. for 30 minutes. The preserved endophytic fungus of mangrove plants, Aspergillus dwarf clavus CCTCC NO: M206063, was transferred to the slant medium and cultured at 32°C for 5 days. Shake flask culture: Prepare medium according to the above seed medium formula, pack into 500 Erlenmeyer flask...

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Abstract

The gram Liewei mellow preparation method and in the system anti-acetylcholin esterase medicine's application, relates to one kind of gram Liewei to be mellow, and provides one kind to originate from the mangrove forest endogenous fungus secondary metabolite gram Liewei mellow preparation method and the application. The preparation method is: dwarf rods Aspergillus BYY-1 seed culture; submerged fermentation; broth filter strains; concentrated filtrate decompression after repeatedly using organic solvent extraction, collection and decompression organic phase was concentrated extracts; Extracts of the silica gel column chromatography and recrystallization obtained after refining grams Levy alcohol. Levy alcohol grams of acetylcholinesterase inhibition with notable is a reversible inhibitor of acetylcholinesterase, can be used for the treatment of Alzheimer's and other associated with a lack of acetylcholine diseases drugs.

Description

technical field [0001] The present invention relates to a kind of clavatol (clavatol), relate in particular to a kind of preparation method that comes from mangrove endophytic fungi-Aspergillus dwarf clavatanicus BYY-1 (A.clavatonanicus) fungal metabolite clavatol compound, and The new application of the compound in the preparation of medicines for treating Alzheimer's disease and other diseases related to acetylcholine deficiency. Background technique [0002] Alzheimer's disease is a neurodegenerative disease, the incidence rate is about 10% in the elderly population aged 65-69, and the prevalence rate in the population over 85 is about 47%, and it is still increasing. Alzheimer's disease seriously endangers the health and quality of life of the elderly. After the disease, the patient will gradually lose the ability of learning and memory, resulting in serious obstacles to self-care and death. [0003] Alzheimer's disease or Alzheimer's Disease (Alzheimer's Disease, AD) i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/26A61K31/122A61P25/28C12R1/66
Inventor 郑忠辉黄珊珊杜希萍黄耀坚宋思扬沈月毛
Owner XIAMEN UNIV
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