Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Reagent for assaying antigen and method of assaying antigen

A technology for antigens and reagents, which is applied in the field of reagents for the determination of antigens and the field of antigen determination. It can solve the problems of different degrees of deviation between the measured value and the true value, different reactivity, and errors in the measurement results, and achieve simple and stable quality management. , Excellent stability and easy measurement

Active Publication Date: 2007-12-12
SEKISUI MEDICAL CO LTD
View PDF3 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, there are two kinds of fPSA and PSA-ACT that can be measured by immunoassay. At present, the kits for measuring the total amount of PSA in more than 20 kinds of serums that have been practically used in Japan have the following problems: due to the different liquids to be tested, other The degree of deviation between the measured value and the true value is different
[0004] That is, in the immunological method, in the so-called immunoagglutination assay using the agglutination reaction using latex or the like, the antigen-antibody reaction proceeds in one stage, so there is a problem that the reactivity of the monoclonal antibody to PSA with fPSA The monoclonal antibody to PSA has a different reactivity with PSA-ACT, and as a result, an error occurs in the measurement result

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Reagent for assaying antigen and method of assaying antigen
  • Reagent for assaying antigen and method of assaying antigen
  • Reagent for assaying antigen and method of assaying antigen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0081] [Manufacture of Monoclonal Antibody]

[0082] 1. Immunity

[0083] (1) Immunogen

[0084] Purified PSA derived from human semen (manufactured by SCIPAC, Code No. P117-7, purity 96%) was used as an immunogen. Purified PSA was used after dialysis against PBS.

[0085] (2) Immunization method

[0086] The above PSA solution and CFA (Complete Freund's Adjuvant: manufactured by GIBCO) were mixed and emulsified at a ratio of 1:1, and 25 μm PSA / mouse was subcutaneously administered to the back of 6-week-old female Balb / C mice. Three booster immunizations were performed at intervals of 2 weeks, and a PSA solution (25 μm / mouse) was intraperitoneally administered to mice three days before cell fusion.

[0087] 2. Cell Fusion

[0088] The spleens of the above-mentioned mice immunized with PSA were excised, and spleen cells were taken out. The spleen cells and mouse myeloma cells SP2 / O-Ag14 were mixed at a ratio of 6:1, and the cells were fused in the presence of 50% polyethy...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
molecular weightaaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

It is intended to provide a reagent and an assay method whereby an antigen can be conveniently and accurately assayed without resorting to any special antibody. Using two or more carriers having different particle sizes, a carrier having a smaller particle size is sensitized with at least one monoclonal antibody, which is selected from among three monoclonal antibodies being reactive with both of a free antigen and a complex antigen and having different recognition sites from each other, while a carrier having a larger particle size is sensitized with the remainder monoclonal antibodies, thereby providing an assay reagent and an assay method whereby the reactivities of a free antigen and a complex antigen can be controlled.

Description

technical field [0001] The present invention relates to an assay reagent for assaying antigen by immunoagglutination reaction and an antigen assay method. Specifically, when an antigen is measured by the latex immunoturbidimetric method (LTIA method), the complex type antigen (such as PSA) formed by regulating the free type antigen (such as fPSA) and the coexisting substance in the test object forms a complex. The reactivity of -ACT) is used to measure the antigen assay reagent and assay method. Background technique [0002] Prostate cancer is a malignant disease that occurs in men, and especially in the United States and Japan, it is a tumor that is rapidly increasing. Prostate cancer is a tumor that proliferates slowly, and radiation therapy and antiandrogen therapy are likely to be effective. Due to these characteristics, early detection is an important issue. [0003] Here, prostate specific antigen (hereinafter abbreviated as PSA) secreted by prostate epithelial cells...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/577G01N33/574
CPCG01N33/54333G01N33/57434G01N33/54313G01N33/574G01N33/577
Inventor 本庄由纪秋元优夏小谷一夫矢后弘和松尾正直宫崎修斋藤和典
Owner SEKISUI MEDICAL CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com