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Highly effective lily bulblet inducement culture method

A technology of small bulbs and lilies, which is applied in the field of efficient production of lily seed balls, can solve problems such as not being able to meet market demand, and achieve the effects of high consistency of bottle production time, complete structure, and shortened breeding time

Inactive Publication Date: 2010-05-19
YUNNAN YUNKE FLOWER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the cultivation process of lily tissue culture bulb seedlings, the existing reports all adopt the culture method of tissue culture seedling proliferation or directly generating small seed bulbs in test tubes, and its proliferation rate is below 100,000 grains / year, but still cannot Meet market demand

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. It is preferred to use the bulbs of the lily variety "Siberia" as explants for cut flowers that have been tested to be free from diseases and insect pests, virus-free, and healthy;

[0040] 2. Rinse the bulbs under running water, and peel off the scales; when peeling off the scales, try to pull the lower part of the scales to peel off, so as to ensure that each scale has a basal tissue; fully shake and clean with washing powder water; use 0.1% Soak in mercuric chloride solution for 40 minutes; soak in 2% sodium hypochlorite solution for 15 minutes; rinse with sterile water twice, lay flat on inoculation paper to filter dry;

[0041] 3. Embryoid body induction culture: Under sterile conditions, inoculate the sterilized scale slices into the following induction medium with the unit of mg / L for cultivation, at a temperature of 23±3°C, and a light time of 10h / L d, cultured under the environmental conditions with light intensity of 2000Lx, observed at any time, and elimin...

Embodiment 2

[0071] 1. It is preferred to use the bulbs of the lily variety "Tiber" as explants for cut flowers that have been tested to be free from diseases and insect pests, virus-free, and healthy;

[0072] 2. Rinse the bulbs under running water, and peel off the scales; when peeling off the scales, try to pull the lower part of the scales to peel off, so as to ensure that each scale has a basal tissue; fully shake and clean with washing powder water; use a concentration of Soak in 0.2% mercuric chloride solution for 30 minutes; soak in 3% sodium hypochlorite solution for 15 minutes; rinse with sterile water for 3 times, lay flat on the inoculation paper to filter dry;

[0073] 3. Embryoid body induction culture: Under sterile conditions, inoculate the sterilized scale slices into the following induction medium with the unit of mg / L for cultivation, at a temperature of 23±3°C, and a light time of 12h / L d, cultured under the environmental conditions with light intensity of 1500Lx, obser...

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Abstract

The invention provides a high active induction culture method for lily bulbil which is characterized in that: through an induction culture of embryoid and tissue enrichment culture of embryoid tissue,a relative high rapid propagation velocity is obtained; then through an differentiation culture of bulb-lets and an intumescence culture of bulb-lets, transplanting bulb-lets of a circumferential diameter bigger than 3cm are obtained. The growth rate of the bulb-lets of the invention can reach more than one million grains per years which is ten times that of conventional methods. With a large number of small bulb-lets obtained, the ratio of bulb-lets with large specification and large grain size is increased. The invention optimizes the culture procedures of preferential lily seedball, shortens the propagation time of preferential lily seedball; furthermore, the field planting survival rate of bulb-lets is high and the production cost is low; the invention is suitable for the scale rapidpropagation production of cut lily elite.

Description

technical field [0001] The invention relates to a method for efficiently producing lily bulbs in a test tube, and belongs to the technical field of plant bulb breeding. Background technique [0002] Lily is a perennial herbaceous bulb plant of the genus Lilium in the family Liliaceae. It is a world-renowned ornamental flower and is widely used in flower decoration. In the past ten years, with the improvement of people's living standards in our country, a large number of excellent ornamental cultivars have been introduced into my country from abroad, and have become high-grade cut flowers in the flower market in our country. The low reproductive coefficient of lily is a prominent problem in the production of lily bulbs. Most of the bulbs of cut lilies cultivated in large areas in my country are imported from the Netherlands. The quality and quantity of bulbs has become an important limiting factor in the production of cut lily in my country. [0003] Lilies are mainly prop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 屈云慧陈卫民张婷李进昆张艺萍
Owner YUNNAN YUNKE FLOWER
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