Cryopreservation solution and cryopreservation method for immune cells
A technology of immune cell and cryopreservation method, which is applied in the field of immune cell cryopreservation solution, which can solve the problems of cytotoxicity, bacteria and viruses with uncertain components, and achieve the effects of not easy aging of cells, less damage, and large number of cell proliferation
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[0018] Below in conjunction with the examples, the specific implementation of the present invention will be further described in detail. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.
[0019] According to the first aspect of the embodiments of the present invention, there is provided an immune cell cryopreservation solution, including DMSO, ethylene glycol, sucrose, polyvinylpyrrolidone, hydroxyethyl starch, glucose, and a cell stabilizer.
[0020] As a preference, each 100mL cryopreservation solution contains DMSO 6.4mL, hydroxyethyl starch 45.6mL and cell stabilizer 34mL.
[0021] Preferably, the cell stabilizer includes glycerol and human serum albumin.
[0022] Preferably, methylcellulose and phosphate-buffered saline PBS are also included.
[0023] According to a second aspect of the embodiments of the present invention, there is provided a method for cryopreserving immune cells, co...
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