Cryopreservation solution and cryopreservation method for immune cells

A technology of immune cell and cryopreservation method, which is applied in the field of immune cell cryopreservation solution, which can solve the problems of cytotoxicity, bacteria and viruses with uncertain components, and achieve the effects of not easy aging of cells, less damage, and large number of cell proliferation

Inactive Publication Date: 2019-06-11
WUHAN PROCELL LIFE SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The commonly used cell cryopreservation scheme is to use DMSO as the cryoprotectant and add bovine serum at the same time. This scheme can be used for the preservation of scientific research products, but there are problems of cytotoxicity, uncertain composition, and possible introduction of pollution sources such as bacteria and viruses.

Method used

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Examples

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Embodiment Construction

[0018] Below in conjunction with the examples, the specific implementation of the present invention will be further described in detail. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0019] According to the first aspect of the embodiments of the present invention, there is provided an immune cell cryopreservation solution, including DMSO, ethylene glycol, sucrose, polyvinylpyrrolidone, hydroxyethyl starch, glucose, and a cell stabilizer.

[0020] As a preference, each 100mL cryopreservation solution contains DMSO 6.4mL, hydroxyethyl starch 45.6mL and cell stabilizer 34mL.

[0021] Preferably, the cell stabilizer includes glycerol and human serum albumin.

[0022] Preferably, methylcellulose and phosphate-buffered saline PBS are also included.

[0023] According to a second aspect of the embodiments of the present invention, there is provided a method for cryopreserving immune cells, co...

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Abstract

The invention provides a cryopreservation solution and cryopreservation method for immune cells. The cryopreservation solution comprises DMSO, ethylene glycol, sucrose, polyvinylpyrrolidone, hydroxyethyl starch, glucose and a cell stabilizing agent. The cryopreservation solution for the immune cells provided by the invention can effectively protect the immune cells against freezing damage, has high safety and little damage to the cells, and can improve a resuscitation survival rate of the immune cells; the resuscitated cells have high viability and a large proliferation quantity, and are not prone to aging; and physiological functions and biological characteristics of the immune cells after resuscitation are guaranteed, the microbiological detection indicators meet requirements, the survival time of the immune cells is prolonged, and loss of surface antigens of the immune cells is reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically, to an immune cell cryopreservation solution and an immune cell cryopreservation method. Background technique [0002] Cell cryopreservation is a technique in which cells are placed in a low temperature environment to reduce cell metabolism for long-term storage. Cell cryopreservation is one of the main methods of cell preservation, which plays a role in cell preservation. Cell cryopreservation is one of the main methods of cell preservation. Using cryopreservation technology to store cells in -196°C liquid nitrogen at low temperature can temporarily remove the cells from the growth state and preserve their cell characteristics, so that the cells can be revived for experiments when needed. Moreover, moderately preserving a certain amount of cells can prevent the cells from being lost due to contamination of the cells being cultured or other accidents, which plays a role in cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 冷毅斌吴亭
Owner WUHAN PROCELL LIFE SCI & TECH CO LTD
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