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Nanoparticles comprising rna ligands

A technology of nanoparticles and ligands, applied in the field of nanoparticles

Inactive Publication Date: 2008-05-14
MIDATECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been observed that although knockdown of target genes by siRNA is not permanent, siRNA transfection alone can lead to prolonged repression of target proteins in parental as well as progeny cells (Tuschl, 2001)
However, delivery of siRNA remains problematic in the art

Method used

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  • Nanoparticles comprising rna ligands
  • Nanoparticles comprising rna ligands
  • Nanoparticles comprising rna ligands

Examples

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Embodiment Construction

[0073] The Her-2 / neu oncogene and its encoded product p185Her-2 / neu belong to the epithelial growth factor receptor tyrosine kinase (Bargmann et al., 1986). The HER receptor family consists of four transmembrane tyrosine kinases: EGFR (also known as Her-1 or erbB-1), erbB-2 (Her-2), erbB-3 (Her-3) and erbB-4 ( Her-4) composition. The Her-2 / neu signaling pathway is known to play a key role in cell growth and differentiation, malignant transformation and resistance to chemotherapeutic agents (Yarden & Sliwkowski, 2001). Her-2 / neu is overexpressed in about one-third of human breast or ovarian cancer cases, and its overexpression is associated with poor prognosis (Berchuck et al., 1990).

[0074] Many attempts have been made to inhibit Her-2 / neu expression in cancer cells as a potential therapeutic approach. Humanized monoclonal antibodies against Her-2 / neu (trastuzumab or Herceptin) are effective in Her-2 / neu-overexpressing metastatic cancers (Mendelsohn & Baselga, 2000; Baselg...

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Abstract

Materials and methods are provided for making nanoparticles having a core including metal and / or semiconductor atoms, which core is covalently linked to a plurality of ligands comprising a RNA ligand. The RNA ligands may include siRNA or miRNA. Also provided are uses of these nanoparticles in therapy and diagnosis.

Description

technical field [0001] The present invention relates to nanoparticles, and more particularly to nanoparticles containing RNA ligands such as small interfering RNA (siRNA) and microRNA (miRNA), and their use in various applications. Background technique [0002] Small RNA molecules have been found to play multiple roles in regulating gene expression. These effects include directed degradation of mRNA by small interfering RNA (siRNA), post-transcriptional gene silencing (PTG), developmentally-controlled sequence-specific translational repression of mRNA by microRNA (miRNA), and directed transcriptional gene silencing. RNAi activity limits transposon transfer and provides antiviral defense (Pal-Bhadra et al., 2004). A role for the RNAi machinery and small RNAs in the targeting of heterochromatin complexes and epigenetic gene silencing at specific chromosomal loci has also been demonstrated (Verdel et al., 2004). Double-stranded RNA (dsRNA)-dependent post-transcriptional silen...

Claims

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Application Information

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IPC IPC(8): C12N15/88A61K47/48A61K49/00A61K51/12A61K49/18G01N33/58C12N15/11C12N15/113
CPCC12N2310/351C12N2310/14C12N2320/12C12N15/1138B82Y15/00A61K49/0063B82Y5/00A61K49/1818C12N15/111A61K51/1255
Inventor T·W·拉德迈克K·古玛M·马丁-洛马斯S·佩纳德斯R·欧杰达A·G·巴雷特斯
Owner MIDATECH LTD
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