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Infrared test approach for venomous harmful acute toxicity algae
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An acute toxicity and infrared testing technology, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of expensive luminescent bacteria, natural water algae testing, and the need for AC power, etc., to achieve stable test results.
Inactive Publication Date: 2013-01-23
CHONGQING UNIV
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The characteristics of the luminescent bacteria method are: high test sensitivity, reaching 0.01-50 mg·L-1, fast test time, an average of 5 minutes; the disadvantage is that the luminescent nature of different luminescent bacteria is different, the operation during detection is cumbersome, and the test instrument is heavy Need AC power, luminescent bacteria are expensive, etc.
However, there are no reports on the acute toxicity of toxic and harmful substances tested by natural water algae at home and abroad, and there is no report on the use of infrared thermometers to test microbial infrared radiation.
Therefore, the search did not find the closest comparative literature related to the research of this method
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[0020] The method of the present invention can perform qualitative and quantitative tests on the acute toxicity of organic poisons, heavy metals, pesticides, etc. with known component contents, and can also perform electroplating wastewater, wastewater from formaldehyde production, coking wastewater, soil pollution, drinking source water, and sewage factory sludge, sewage plant effluent, etc.; formalin-soaked tripe, pig tendon, beef tendon, duck intestine, dried squid, dried sea cucumber, dried cuttlefish, etc.; tomatoes, cucumbers, cauliflower, and Chinese cabbage that have been treated with pesticides , Lianhuabai, cowpea, Xinjiang grapes, strawberries, cherries and other acute toxicity test.
[0021] Referring to Fig. 1, the infrared test method of toxic and harmful substance acute toxicity algae of the present invention specifically comprises the following steps:
[0022] 1 Preparation before the test
[0023] 1.1 Sensitive algae preparation: Sensitive algae with Ceratum ...
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Abstract
The invention provides an acute toxicity algae infrared testing method of toxic and harmful substances, which adopts the sensitive algae liquid with the chlorophyll content not less than 4.0mg.L-1 and the toxicity test factors of a portable infrared thermometer. The contrast toxicity test is carried out by selecting the processing and concentration design of a test sample, the response dose and the minimum response concentration of the sensitive algae to the sample are determined by the sample dilution concentration and the adding amount of a first-appeared temperature response during the dilution concentration test of using different samples for response temperature difference and response time so as to determine the toxicity of the sample. The sensitivity of the method of the invention can achieve the sensitivity of a luminous bacteria method, the average testing time of the method and the average testing time of the luminous bacteria method are similar, the testing effect ofthe acute toxicity algae infrared testing method of the toxic and harmful substances is the same with the testing effect of the luminous bacteria method. The invention is characterized by convenient test, low cost, strong specificity, field test and so on.
Description
technical field [0001] The invention relates to a method for testing the acute toxicity of organic poisons, heavy metals and pesticides, in particular to a method for testing the acute toxicity of toxic and harmful substances with sensitive algae. The invention belongs to the technical field of environmental protection, food safety monitoring and the like. [0002] Scope of application: This method is suitable for organic poisons, heavy metals, pesticides; electroplating wastewater, wastewater from the production of formaldehyde, coking wastewater, polluted soil agriculture, polluted drinking water, sewage plant sludge, sewage plant effluent, etc.; formalin soaked hair tripe, pig tendon, beef tendon, duck intestine, dried squid, dried sea cucumber, dried cuttlefish, etc.; tomatoes, cucumbers, cauliflower, Chinese cabbage, lotus white, cowpea, Xinjiang grapes, strawberries, cherries, etc. Toxicity testing. Background technique [0003] In the prior art, electroplating waste...
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