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Cinobufacini extract and preparation method thereof

A technology of extract and cinobufagin, which is applied in the direction of drug combination, pharmaceutical formula, medical preparations containing active ingredients, etc., can solve the problems of toxic side effects, reduced curative effect, low content of active ingredients of invalid substances, etc.

Inactive Publication Date: 2011-04-06
SICHUAN ACAD OF CHINESE MEDICINE SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, cinobufacin injections, oral liquids, and tablets have been used clinically, but in clinical applications, it is found that due to the high content of invalid substances (impurities) and low content of active ingredients in the extract, the product has certain toxic and side effects and the curative effect is reduced.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Take 1kg of dried toad skin, add 10L water to decoct for 45 minutes, that is, the first decoction; after the first decoction, add 5L water to decoct the dregs, that is, the second decoction; combine the two decoction liquids, filter, and cool the filtrate to 30°C , adsorbed on D101 macroporous adsorption resin column, first eluted with 6L water, then 5L 70% ethanol, collected the ethanol eluate, recovered the ethanol in the eluate, concentrated, dried, and obtained cinobufacin extraction 16.5g of the product, the total amount of sinobufonitoxin in the extract was 31.2mg, and the total amount of redibufagenin was 20.8mg.

Embodiment 2

[0064] Take 1kg of dried toad skin, add 7L water to decoct for 45 minutes, that is, the first decoction; ℃, adsorb on AB-8 macroporous adsorption resin column, first elute with 7L of water, then elute with 6L of 60% ethanol, collect the ethanol eluate, recover the ethanol in the eluate, concentrate, dry, and get Hua 15.3g of bufobufoni extract, the total amount of sinobufonitoxin in the extract is 22.9mg, and the total amount of redibufagenin is 15.6mg.

Embodiment 3

[0066] Take 1kg of dried toad skin, add 10L of water, and decoct for 30 minutes, that is, the first decoction; ℃, adsorbed on NKA-9 macroporous adsorption resin column, first eluted with 5L water, then 4L 80% ethanol, collected the ethanol eluate, recovered the ethanol in the eluate, concentrated, dried, Dehua 18.4g of bufobufoni extract, the total amount of sinobufonitoxin in the extract is 36.5mg, and the total amount of redibufagenin is 24.2mg.

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Abstract

The invention discloses a Chinese toad bufotoxin extract and a preparation method thereof. The extract contains the main effective components of cinobufagin with the content more than 0.15 percent and resibufogenin with the content more than 0.10 percent. The extract is made from dry toad skin and is prepared by the technologies of poaching, column chromatographic separation, eluting, condensing,drying, etc. The invention effectively removes useless substances (impurity) in the extract, is enriched in effective component and achieves the goals of improving the curative effect and reducing the toxic and side effects.

Description

technical field [0001] The invention relates to an extract and a preparation method thereof, in particular to a cinobufacin extract and a preparation method thereof. Background technique [0002] Cinobufacin extract is a brown-yellow extract obtained by extracting and purifying the dried skin of Bufo bufogargarizans Cantor, an animal of the Bufonidae family; , chronic hepatitis B and other diseases, its physiologically active substances are mainly bufotoxins and their hydrolyzed products bufageins, bufoteinines, and other compounds such as cholesterol. [0003] At present, cinobufacin injections, oral liquids, and tablets have been used clinically, but in clinical applications, it is found that due to the high content of invalid substances (impurities) and low content of active ingredients in the extract, the product has certain toxic and side effects and the curative effect is reduced. . Contents of the invention [0004] One object of the present invention is to disclo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/56A61P1/16A61P29/00A61P31/20A61P35/00A61K35/65
Inventor 张爱军谭正怀
Owner SICHUAN ACAD OF CHINESE MEDICINE SCI
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