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Immunological assay reagents and assay method

一种试剂、待分析的技术,应用在免疫分析试剂和分析领域,能够解决没有参加、没有达到等问题

Inactive Publication Date: 2009-06-03
MITSUBISHI CHEM MEDIENCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, even if these studies are beneficial to the manufacturers and suppliers of latex particles, there is no room for participation by those who receive the supply of latex particles
Although it has been conceived to allow a stabilizer composed of a surfactant or a polysaccharide to coexist in the preservation solution of the latex particles after the antibody (or antigen) is attached, the actual situation is not to the extent that the above-mentioned problems can be solved at once.

Method used

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  • Immunological assay reagents and assay method

Examples

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Embodiment 1

[0048] Example 1: Determination of soluble fibrin

[0049] (1) Preparation of soluble fibrin antibody conjugated biotin solution

[0050] Monoclonal antibody FM No. 43-1 secreted by hybridoma FM No. 43-1 described in WO95 / 12617 was used as the anti-soluble fibrin (SF) monoclonal antibody. The above-mentioned hybridoma has been deposited domestically at the Patent Organism Depositary Center of the National Institute of Advanced Industrial Science and Technology [(former) Industrial Technology Research Institute of Biotechnology (Address: 305-8566 Tsukuba City, Ibaraki Prefecture, Japan) since October 27, 1993. Higashi 1-chome 1 Fandi 1 Central No. 6)], which was transferred to the international depository on October 27, 1994. The international accession number (the domestic accession number following the international accession number []) is FERM BP-4846 [FERM P-13925].

[0051] Specifically, the monoclonal antibody ascites against soluble fibrin (SF) was prepared in the same...

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Abstract

The invention relates to immunological assay reagents and assay method. Immunological assay reagents comprise a composition containing a conjugate composed of an antigen or an antibody against the subject to be assayed and biotin bonded thereto and another composition containing avidin-binding particles, wherein biotin is bonded to the antigen or antibody in the above-described conjugate in such an amount that it would not undergo agglutination together with the above-described avidin-binding particles in the absence of the subject to be assayed; and an immunological assay method which comprises bringing a sample to be assayed into contact with a conjugate composed of an antigen or an antibody against the subject to be assayed and biotin bonded thereto (provided that biotin is bonded to the antigen or antibody in such an amount that it would not undergo agglutination together with the above-described avidin-binding particles in the absence of the subject to be assayed) and avidin-binding particles, and then evaluating the extent of the agglutination caused by the immune complex formed by the subject to be assayed and the conjugate and the avidin-binding particles. By using the above immunological assay reagent and the immunological assay method, a subject to be assayed can be accurately assayed without resort to a procedure of carrying an antibody or an antigen on latex particles (i.e., being free from the autoagglutination of latex particles), thereby achieving a reactivity comparable or even superior to those obtained by the conventional sensitized latex method.

Description

[0001] This application is a divisional application filed on December 11, 2001, the application number is 01804853.6, the title of the invention is the same as the invention patent of the present invention. technical field [0002] The present invention relates to immunoassay reagent and analysis method. In more detail, it relates to an analytical reagent and an analytical method for an agglutination reaction by utilizing an immune reaction. The above-mentioned "analysis" in this specification includes both quantitative or semi-quantitative "measurement" of the amount of the analyte and "detection" of judging the presence or absence of the analyte. Background technique [0003] As one of the methods for quantitatively analyzing trace components of biological fluids, immunoassay methods using antibodies or antigens of substances to be analyzed are commonly used. As the immunoassay means, the immunocolorimetric method and immunoturbidimetric method for optically measuring the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/535
CPCG01N33/54346G01N33/535G01N33/54333
Inventor 中原邦彦泽井时男
Owner MITSUBISHI CHEM MEDIENCE
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