Alpha<sl>-casein and kappa-casein specific antibody, kit and test paper strip

Abstract

The invention discloses an alpha<s1>-casein and kappa-casein specific antibody, a kit and a test paper strip, which aims to provide a rapid specificity detection whether the casein content of raw material dairy product reaches the standard on site, having high resolution factor and shortened detection time. The specific antibody is prepared by the following method: separated serum of rabbit blood sample is collected as feminine control serum when antiserum detection; alpha<s1>-casein solution is mixed with complete Freund's adjuvant to obtain alpha<s1>-casein solution; kappa-casein solution is mixed with complete Freund's adjuvant to obtain kappa-casein mixed solution; two casein solutions are respectively extracted and mixed till complete emulsification to obtain immune injection, and ventral subcutaneous 5-point immune injection is carried out; booster immunization is carried out four times 21 days later after primary immunization; separated serum of venous blood is extracted 7-10 days later after each booster immunization, polyclonal antiserum is collected in blood collection when the titer of the antiserum is detected to be more than 1: 2700, and the specific antibody is obtained by separation and purification.

Description

technical field [0001] The present invention relates to the field of biotechnology, more specifically, relates to an alpha s1 - Casein and kappa-casein specific antibodies (IgG) and kits and test strips. Background technique [0002] At present, the detection methods of raw milk adulteration are limited to traditional physical and chemical reactions and instrumentation models. Although there are advanced instruments such as Foss120 that can quickly detect the adulteration of raw milk, these instruments are generally expensive and require professional operation , is not suitable for the application of small and medium-sized enterprises, and is not suitable for on-site testing. It cannot meet the rapid development of the current dairy industry, and its determination of protein content depends on the determination of nitrogen content, which also lays a hidden danger for adulteration. [0003] Abroad, the research on the detection technology of bovine milk casein started relati...

AI Technical Summary

Problems solved by technology

[0002] At present, the detection methods of raw milk adulteration are limited to traditional physical and chemical reactions and instrumentation models. Although there are advanced instruments such as Foss120 that can quickly detect the adulteration of raw milk, these instruments are generally expensive and require professional operation , is not suitable for the application of small and medium-sized enterprises, let alone on-site testing, and cannot meet the rapid development of the current dairy industry, and its determination of protein content depends on the determination of nitrogen content, which also lays a hidden danger for adulteration.