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Method for enriching albumin from human urine on large scale

An albumin and enrichment technology, applied in the field of enriching human blood albumin, can solve the problems of urban sanitation damage, high collection and transportation costs, unavoidable urine spilling, etc., to reduce collection and processing costs and improve work efficiency Effect

Active Publication Date: 2011-08-17
YANGZHOU AIDEA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the concentration of albumin in human urine is very low, which is only 1 / 2000-4000 of plasma, even if urine is collected in public toilets in cities with high population density, the cost of collection and transportation is very high. Difficult to avoid spilling urine, causing damage to urban sanitation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 makes urine filter pad

[0020] The urine filter pad is composed of a cloth bag for wrapping and anion exchange resin filled in the cloth bag. The production method is to sew a cloth bag with a filter cloth that has good permeability and can firmly wrap the adsorbent particles. The suitable sieve size of the filter cloth is 300 mesh or less, with a pore size of 50 mesh or more, preferably 50-100 mesh sieve size. The shape and size of the filter pad are compatible with the bottom of the urinal or urinal, and can be square, round, polygonal, etc.; the side length or diameter of the filter pad used for the urinal is preferably 10-40cm; The filter pads used in urinals can be divided into two types: one is laid flat on the bottom of the urinal, and its size should be able to fully cover the bottom of the urinal; the other type is installed at the urine outlet of the urinal and can be filled And cover the urine outlet, so that the urine flows through the filter...

Embodiment 2

[0023] Example 2 Making a cylindrical urine filter device

[0024] Choose a plastic tube with a wall thickness of about 1mm, preferably white; the cross-section of the tube is circular, rectangular or polygonal; the circumference of the tube is suitable for the outlet of the urinal or urinal; the height of the tube wall is selected according to the place where it is placed: The filter column in the urinal is preferably cylindrical, and the height should be 2-10cm; the specific size of the filter column installed at the outlet of the urinal is determined according to the shape, size and depth of the outlet, and the height of the tube wall should be 3cm-30cm .

[0025] The upper and lower openings of the urine filtration column are closed with filter cloth or filter membrane, and the filter cloth screen size is preferably 50 mesh; the lumen is filled with anion exchange resin. The materials and pretreatment of anion exchange resin are the same as in Example 1.

Embodiment 3

[0026] Example 3 Test urine filter pad enrich albumin effect

[0027] Using the urine filter pad made by the method of Example 1, the size of the filter pad is 20cm × 30cm, and the filter cloth aperture of the filter pad is 50 orders; the ligand used for the anion exchange resin is [-N + (CH 3 ) 3 ] 2 kg of macroporous anion exchange resin acrylic resin, treated with 2% NaOH, washed with water to neutrality, packed into a filter cloth bag to make a urine filter pad, and placed in a urinal. In the pouring method, pour 200L of the prepared male urine stock solution into the urinal at a roughly uniform flow rate for 30 minutes, and the urine flows through the urine filter pad. Afterwards, take out resin from filter mat, after washing with tap water, pack into chromatographic column, desorb with the acetate buffer (50mM, pH4.5) that contains 1MNaCl again; Collect elution peak 5200ml, measure urine, residual urine, The albumin content and total protein content of the desorption ...

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Abstract

The invention relates to method for enriching albumin from human urine on a large scale, which is especially suitable for large-scale application. The method is realized in a way that: a urine filter is made of an anion exchanger, is put in a urine bucket or a urinal and is used for containing urine, so that the urine can flow through the filter; the shape and size of the urine filter are suitable for those of the urine bucket or the urinal; the urine filter is regularly recovered, the anion exchanger in the urine filter is concentratedly desorbed, ingredients containing albumin are collected, and the anion exchanger is regenerated for later use; and after subsequent purification, the albumin the various indices of which meet the pharmacopoeia requirements can be prepared, and can be usedas a substitute for human plasma source albumin in many fields.

Description

technical field [0001] The present invention relates to a method for enriching human serum albumin from human urine. Background technique [0002] Human serum albumin (HSA) normally exists in human plasma and has very important physiological functions. Human serum albumin extracted from human plasma is an irreplaceable drug for rescue treatment of shock, burns, and trauma, and is widely used in places such as war injuries, industrial accidents, and surgical operations; clinically, human serum albumin is also used for chronic Treatment of nephritis, hepatitis, liver cirrhosis and advanced malignant tumors, malnutrition edema, hypoalbuminemia and other diseases; human albumin is also used as excipient and stabilizer, immune adjuvant for vaccine production, serum-free cells Components of the culture medium, etc.; also used as a drug carrier to endow the drug with better physical and chemical properties. Albumin is also a commonly used and expensive biological reagent. In a la...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/34C07K14/76
Inventor 苗丕渠傅和祥俞恒傅和亮
Owner YANGZHOU AIDEA BIOTECH
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