Method for separating glycoside chemical components from tibetan capillaris

A technology of chemical components and glycosides, which is applied in the field of separating chemical components of glycosides from Capsicum officinalis, and achieves the effect of low cost and short time

Active Publication Date: 2010-09-15
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the above-mentioned method of extracting Yinchen Tibetan

Method used

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  • Method for separating glycoside chemical components from tibetan capillaris
  • Method for separating glycoside chemical components from tibetan capillaris
  • Method for separating glycoside chemical components from tibetan capillaris

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Weigh 20kg of Tibetan capillary herb, dry, crush, infiltrate and swell with 90% ethanol, fill in a percolation barrel with a diameter of 20cm; after soaking for 6 hours, start percolation at a speed of 1-2mL per minute, and receive 3 double the volume to obtain 90% ethanol percolation solution; add 60% ethanol to the percolation barrel, soak for 6 hours, start percolation at a speed of 1-2 mL per minute, receive 3 times the volume, and obtain 60% ethanol percolation Add 30% ethanol to the percolation barrel, soak for 6 hours, start percolation at a speed of 1-2mL per minute, receive 3 times the volume, and obtain 30% ethanol percolation liquid; add water to the percolation barrel , after soaking for 6 hours, start percolating at a speed of 1-2 mL per minute, receive 3 times the volume, and obtain water percolating liquid.

[0023] Respectively recover the ethanol percolation liquid and water percolation liquid under reduced pressure until they have no alcohol smell, and...

Embodiment 2

[0031] Take by weighing 20kg Western Sichuan Swert Sprout Medicinal Material, and the extraction method is the same as in Example 1.

[0032] The chemical components of glycoside monomers obtained from the separation of D101 macroporous resin column are the following 1-3 chemical components of glycosides:

[0033] 1. Gentiopicroside: the structural formula is as follows:

[0034]

[0035] mp.191.9℃~193.8℃; UVλ MeOH nm 212.2, 243.9, 276.9;

[0036] 1 H-NMR (400MHz, DMSO-d 6 )δ: 7.40 (1H, d, J = 1Hz, 3-H), 5.70 (1H, m, 6-H), 5.57 (1H, d, J = 3Hz, 1-H), 5.62 (1H, m, 8-H), 5.19 (2H, m, 9-H); 13 C-NMR (400MHz, DMSO-d 6 )δ: 96.5(C-1), 148.3(C-3), 103.3(C-4), 125.0(C-5), 116.0(C-6), 69.9(C-7), 133.9(C-8 ), 44.4(C-9), 117.7(C-10), 162.7(C-11), 98.8(C-1`), 72.7(C-2), 76.5(C-3`), 69.1(C- 4`), 77.2 (C-5`), 61.0 (C-6`).

[0037] 2. Swertiamarin: the structural formula is as follows:

[0038]

[0039] UV lambda MeOH nm 247.5; IRv KBr cm -1 3390.2, 1694.1, 1618.4; 1 H-NM...

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Abstract

The invention discloses a method for separating glycoside chemical components from tibetan capillaries, comprising three steps of extracting water solution of an tibetan capillaries extract, extracting and crudely separating according to the polarity gradient and separating and purifying by column chromatography. The invention has important significance on the study of the pharmacological effectsof the tibetan capillaries and the research and the production of medicines thereof. By using the method, chemical components are separated into component groups with larger polarity difference, macroporous resin of the glycoside chemical components is separated and the cost is lower in comparison with the column chromatography separation for an extract at the later stage.

Description

technical field [0001] The invention relates to a method for extracting components of Tibetan capillary wormwood, and more specifically relates to a method for separating chemical components of glycosides in Tibetan capillary wormwood. technical background [0002] Tibetan Yinchen is one of the folk herbal medicines commonly used in Tibetan medicine in Tibet, Qinghai, Sichuan, Yunnan and Gansu. It has been used for liver and gallbladder diseases for a long time, and is especially famous at home and abroad for treating acute hepatitis and jaundice. However, the research on the chemical constituents of Tibetan Yinchen is relatively backward. [0003] Chinese Invention Patent (Patent No.: 92108809.4) discloses an extraction process and equipment for effective components of Capillary chinensis and a production method for Capillary cypress tablets. The production feature of the continuous extraction process is that the extract powder is 45-90%, and the adjuvant is 10-55% Tibetan...

Claims

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Application Information

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IPC IPC(8): C07D407/04C07H17/04C07H1/08
Inventor 罗维早阳勇钟国跃覃瑶王昌华周华容王欣
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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