Constructing method of mouse model for RdRp controllable express and in vivo observation
A technology for regulating expression and mouse models, applied in the biological field, can solve the problems of limited application, low reproduction rate, and low transplantation success rate, and achieve the effects of shortening the experimental cycle, overcoming low fertility rate, and strong induction performance
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[0046] The invention provides a method for constructing a mouse model in which the expression of RdRp can be regulated and observed in vivo. First, the RdRp gene was cloned into a specific expression vector, and then the single transgenic mouse with regulated expression of RdRp was crossed with the Cre / Lap double transgenic mouse, and the RdRp / Lap gene with strong reproductive ability, low background expression and strong inducibility was screened. Cre / Lap triple transgenic mice, the selected mice can strictly regulate the expression of RdRp through dox induction, and the single transgenic mice with regulated expression of RdRp can be prepared by transgenic technology, and RdRp is not expressed when the mice are fed normally. After being induced by dox feeding, RdRp began to be expressed in the liver; the effect of RdRp inhibitors could be visually observed by mouse in vivo imaging technology; finally, the population was expanded by cross breeding of RdRp / Cre / Lap triple transge...
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