Molecular marking method for distinguishing Jian carps

A technology of molecular markers and carp construction, applied in biochemical equipment and methods, microbiological measurement/inspection, etc., can solve the problems of multiple hybrid species, hybrid carp germplasm, hybrid species degradation, etc., and achieve the goal of avoiding germplasm hybridization Effect

Inactive Publication Date: 2011-02-16
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

[0003] The purpose of the present invention is to provide a molecular marker method for identifying Jian carp, which helps to solve the problems of mixed carp germplasm, many hybrid species, and hybrid species degeneration, and can identify and separate germplasm from mixed carp germplasm; Simple operation, easy to master, high accuracy

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example

[0042] Obtain the blood of the orthogonal F1 populations of Jian carp, Yellow River carp and Jian carp, extract DNA through the DNA extraction kit of TAKARA company, and use the gel to detect the quality of the extracted DNA; use the provided control and collected samples for PCR amplification increase, and compare the amplified bands. After the amplified bands were sent to Shanghai Yingjun Company for sequencing, the obtained sequences were analyzed for mutant type and wild type through the sequencing map. By comparing with the control, analyze and determine the germplasm resources to be separated.

[0043] Germplasm Separation Based on Orthogonal F1 Mixed Populations of Jian, Yellow River and Jian Carps

[0044] 1. Germplasm separation of Jian carp

[0045] From the pond, the orthogonal F1 generation mixed populations (48 fish) of Jian carp, Yellow River carp and Jian carp were randomly selected, and PIT marker scanning was performed to determine the individual markers of ...

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Abstract

The invention relates to a molecular marking method for distinguishing Jian carps, which comprises the following steps of: selecting a mixed group of the selfing F1 generation of Jian carps and the hybridization F1 generation of Yellow river carp male parent and Jian carp female parent as a research object, randomly selecting 48 carps, respectively sampling blood, and respectively extracting a DNA sample by using a DNA extraction method; carrying out PCR (Polymerase Chain Reaction) amplification by using IGF2a gene intron 2, and taking the extracted DNA sample of the mixed group as a template; detecting the amplified PCR product as 693bp through electrophoresis, and sequencing to obtain the amplified nucleotide sequence; and comparing the sequences according to the result of given wile type basic group C and mutant type basic group CA for contrast after the nucleotide sequence is obtained, and judging the comparison result. The invention has the advantages of separating germ plasm from Jian carps, Yellow river carps and the forward hybridization F1 generation of Jian carps, providing a reference path for treating river germ plasm mixed hybridization and providing a method for breeding improvement to avoid germ plasm mixed hybridization.

Description

technical field [0001] The invention relates to a molecular marker method for identifying Jian carp. Background technique [0002] At present, the phenomenon of germplasm mixing in carp production is serious, how to identify and separate germplasm from mixed germplasm has become an urgent problem. Jian carp is a relatively widespread fish in my country's cultured carp, but the phenomenon of germplasm mixing is relatively serious, including seedling farms, breeding farms and many other links. If we want to identify and separate varieties from the existing situation, it will help to solve the problems of germplasm mixing, many mixed varieties, and the degradation of mixed varieties. The method of separation is called an effective way to solve the problem of germplasm mixing. Contents of the invention [0003] The purpose of the present invention is to provide a molecular marker method for identifying Jian carp, which helps to solve the problems of mixed carp germplasm, man...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 董在杰苏胜彦曲疆奇
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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