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Loop-mediated isothermal amplification (LAMP) reaction primer for detecting subgroup-A avian leukosis virus (ALV-A)

A technology of avian leukosis virus and ring-mediated isothermal, which is applied in the field of detection of subgroup A avian leukemia, can solve the problems of economic loss and infection in the poultry industry, and achieve the effect of high accuracy, strong specificity and high sensitivity

Inactive Publication Date: 2012-01-04
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, due to the lack of effective measures and perfect system for the purification of avian leukosis in our country, disease outbreaks or immunosuppression caused by avian leukosis virus infection or mixed infection of avian leukosis virus and other poultry immunosuppressive disease viruses have occurred from time to time. , the infection of the disease has become more and more serious, causing huge economic losses to the poultry industry

Method used

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  • Loop-mediated isothermal amplification (LAMP) reaction primer for detecting subgroup-A avian leukosis virus (ALV-A)
  • Loop-mediated isothermal amplification (LAMP) reaction primer for detecting subgroup-A avian leukosis virus (ALV-A)
  • Loop-mediated isothermal amplification (LAMP) reaction primer for detecting subgroup-A avian leukosis virus (ALV-A)

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Embodiment 1

[0018] 1. Material method

[0019] 1 Materials and methods

[0020] 1.1 virus

[0021] J subgroup avian leukemia HPRS-103 strain (GenBank accession number: Z46390), A subgroup avian leukemia RAV-1 strain (GenBank accession number: M19113), B subgroup avian leukemia RAV-2 strain (GenBank accession number: M14902) , C subgroup avian leukemia RAV-49 strain (GenBank accession number: V01197), E subgroup avian leukemia RAV-0 strain (GenBank accession number: M12172), avian infectious bursal disease supervirulent Gx strain (GenBank accession number No.: AY444873), chicken infectious anemia virus (CAV) M9905 strain, and avian reticuloendothelial disease virus HLJR0901 strain are preserved by the poultry immunosuppressive disease research group of Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (also available from China Veterinary Drug Monitoring purchased). Avian influenza virus (AIV), Newcastle disease virus (NDV), chicken infectious bronchitis vir...

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Abstract

The invention discloses a loop-mediated isothermal amplification (LAMP) reaction primer for detecting subgroup-A avian leukosis virus (ALV-A). The primer consists of a pair of outer primers, a pair of inner primers and a pair of ring primers, wherein the outer primers are shown in SEQ ID NO.1 and SEQ ID NO.2; the inner primers are shown in SEQ ID NO.3 and SEQ ID NO.4; and the ring primers are shown in SEQ ID NO.5 and SEQ ID NO.6. On the basis of the designed LAMP primer, an LAMP detection method for ALV-A subtypes is successfully established. By means of the method, the ALV-A can be distinguished from ALV-J, ALV-B, ALV-C and ALV-E, and is not subject to nonspecific reaction together with other common poultry infectious diseases. The ALV-A LAMP detection method established in the inventionensures high accuracy, high sensitivity and strong specificity.

Description

technical field [0001] The invention relates to a primer for detecting animal diseases, in particular to a specific primer for loop-mediated isothermal amplification reaction (LAMP) for detecting A subgroup avian leukemia virus, and belongs to the detection field of A subgroup avian leukemia virus. Background technique [0002] Avian leukemia is an immunosuppressive disease caused by avian leukosis virus (ALV) and characterized by a variety of tumors. [0003] At present, due to the lack of effective measures and perfect system for the purification of avian leukosis in our country, disease outbreaks or immunosuppression caused by avian leukosis virus infection or mixed infection of avian leukosis virus and other poultry immunosuppressive disease viruses have occurred from time to time. , the infection of the disease has become more and more serious, causing huge economic losses to the poultry industry. [0004] According to the virus neutralization test, virus interference ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/70C12N15/11
Inventor 王笑梅王永强高玉龙高宏雷秦立廷祁小乐
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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