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Breeding method of japonica photoperiod/thermo-sensitive genic male sterile lines

A photo-thermosensitive sterility, japonica technology, which is applied in the fields of botanical equipment and methods, plant genetic improvement, horticulture, etc. problems such as low difficulty, to achieve the effect of eliminating gene drift, reducing risk and improving reproductive yield

Inactive Publication Date: 2011-12-28
INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the defects and problems in the prior art that are not suitable for the reproduction of japonica-type photothermo-sensitive genetic male sterile lines, are relatively risky, and are difficult to operate, and provide a japonica-type photothermal Propagation of sensitive genic male sterile lines, less risky, and less difficult to operate japonica-type photothermosensitive genic male sterile lines

Method used

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Embodiment

[0034] A kind of propagation method of japonica type photothermosensitive genetic male sterile line, this propagation method comprises the following steps successively:

[0035] The first step: individual plant selection process: first sow the japonica-type photothermosensitive GMS line N5088S on May 10, and then select 300 individual plants as the initial individual plants according to the morphological characteristics of the plants and leaves for pot planting. And grow under natural light and temperature conditions and strict and standardized fertilizer and water management;

[0036] The second step: long-term low-temperature identification of sterility process: when the above-mentioned initial single plant enters the pistil formation period under natural light and temperature conditions, it is first moved into a sunlight-type artificial climate box for treatment until the terminal meiosis of pollen mother cells, The treatment time is 12 days, the sunlight time is more than ...

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Abstract

The invention discloses a breeding method of japonica photoperiod / thermo-sensitive genic male sterile lines. The method sequentially comprises the following steps of: single strain selection process, sterility identification process through linear light at a low temperature, fertility identification process through short light at a high temperature, core seed production process and breeder's stock and stock production process. In the sterility identification process through linear light at the low temperature, the daily sunlight time is more than 14.5 hours; the daily average temperature is 24 DEG C; and the day and night temperature amplitude is 20 to 28 DEG C. In the fertility identification process through short light at the high temperature, the daily sunlight time is less than 13 hours; the daily average temperature is 28 DEG C; and the day and night temperature amplitude is 25 to 30 DEG C. The core seed production process comprises a core rice stump preservative method in HannanProvince or a greenhouse core rice stump preservative method in middle and lower reaches of the Yangtze River, wherein the height of stump cutting in the breeding method is 20cm. The breeding method eliminates genetic drift phenomena in a japonica photoperiod / thermo-sensitive genic male sterile line breeding process and solves the problems of low yield, high risk and difficulty in operation in the japonica photoperiod / thermo-sensitive genic male sterile line breeding process.

Description

technical field [0001] The present invention relates to a kind of breeding method of photothermosensitive male sterile line, especially relates to a kind of breeding method of japonica photothermosensitive male sterile line, especially suitable for overcoming the heredity in the breeding of japonica photothermosensitive male sterile line Drift phenomenon and enhancement of reproductive yield of japonica photothermosensitive genetic male sterile lines. Background technique [0002] Since Shi Mingsong discovered photosensitive genic male sterile rice in Hubei Province in 1973, more than 30 photothermosensitive genic male sterile lines have been bred in Hubei Province. When these sterile lines were bred, the starting point temperature all met the approval and popularization and application standards, but in the seed production, the photothermosensitive genic male sterile line seeds were still produced according to the conventional original seed, original seed, and production se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G16/00A01H1/02A01G22/22
Inventor 游艾青胡刚刘凯杨国才陈志军李三和周雷
Owner INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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