Breeding method of japonica photoperiod/thermo-sensitive genic male sterile lines
A photo-thermosensitive sterility, japonica technology, which is applied in the fields of botanical equipment and methods, plant genetic improvement, horticulture, etc. problems such as low difficulty, to achieve the effect of eliminating gene drift, reducing risk and improving reproductive yield
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[0034] A kind of propagation method of japonica type photothermosensitive genetic male sterile line, this propagation method comprises the following steps successively:
[0035] The first step: individual plant selection process: first sow the japonica-type photothermosensitive GMS line N5088S on May 10, and then select 300 individual plants as the initial individual plants according to the morphological characteristics of the plants and leaves for pot planting. And grow under natural light and temperature conditions and strict and standardized fertilizer and water management;
[0036] The second step: long-term low-temperature identification of sterility process: when the above-mentioned initial single plant enters the pistil formation period under natural light and temperature conditions, it is first moved into a sunlight-type artificial climate box for treatment until the terminal meiosis of pollen mother cells, The treatment time is 12 days, the sunlight time is more than ...
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