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Application of puer tea for eliminating formaldehyde biological damage

A technology for eliminating formaldehyde and Pu'er tea, applied in the field of Pu'er tea, can solve the problem that the biological hazard of formaldehyde in Pu'er tea has not been discovered, and achieve the effect of avoiding biological toxicity

Inactive Publication Date: 2011-05-11
普洱市人民政府茶产业发展办公室科技服务中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] But so far, Pu'er tea is used to eliminate the biological hazards of formaldehyde, and no relevant information has been found.

Method used

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  • Application of puer tea for eliminating formaldehyde biological damage
  • Application of puer tea for eliminating formaldehyde biological damage
  • Application of puer tea for eliminating formaldehyde biological damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Inoculate 1x10 5 -1x10 6 Put mouse embryonic fibroblasts on glass slides in six-well cell culture plates, use DMEM+10% FBS medium, and culture them in a 37°C, 5% CO2, 3% O2 ​​incubator for 12 to 24 hours. Pu-erh tea with a final concentration of 0.05 mg / ml was added for co-cultivation for 6 hours, and the blank control was co-cultivated without Pu-erh tea. Then, add 100-300 μmol / L formaldehyde to the cells of Pu'er tea protection group and blank control group respectively. At four time points of 30min, 1hr, 3hr, and 5hr, the morphological changes of cells were observed by differential interference imaging (DIC) microscopy, and Annexin V fluorescent staining was performed.

Embodiment 2

[0040] Inoculate 1x10 5 -1x10 6Put mouse embryonic fibroblasts on glass slides in a six-well cell culture plate, use DMEM+10% FBS medium, and culture in a 37°C, 5% CO2, 3% O2 ​​incubator for 12-24 hours, Pu-erh tea with a final concentration of 0.25 mg / ml was added for co-cultivation for 6 hours, and the blank control was co-cultivated without Pu-erh tea. Then, add 100-300 μmol / L formaldehyde to the cells of Pu'er tea protection group and blank control group respectively. At four time points of 30min, 1hr, 3hr, and 5hr, the morphological changes of cells were observed by differential interference imaging (DIC) microscopy, and Annexin V fluorescent staining was performed.

Embodiment 3

[0042] Inoculate 1x10 5 -1x10 6 Put mouse embryonic fibroblasts on glass slides in six-well cell culture plates, use DMEM+10% FBS medium, and culture them in a 37°C, 5% CO2, 3% O2 ​​incubator for 12 to 24 hours. Pu-erh tea with a final concentration of 0.05 mg / ml was added for co-cultivation for 24 hours, and the blank control was co-cultivated without Pu-erh tea. Then, add 100-300 μmol / L formaldehyde to the cells of Pu'er tea protection group and blank control group respectively. At four time points of 30min, 1hr, 3hr, and 5hr, the morphological changes of cells were observed by differential interference imaging (DIC) microscopy, and Annexin V fluorescent staining was performed.

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Abstract

The invention relates to novel application of puer tea, in particular to application of the puer tea for eliminating formaldehyde biological damage. In the invention, a deep experimental research is carried out, and a wild type mouse embryo fibroblast (MEF) is utilized to test the killing action of the formaldehyde to normal cells and the protection action of the puer tea to the normal cells; the cells are massively dead after being treated with 100-300 micron mol / L formaldehyde; however, only a few cells under the protection of puer tea extract which is added in advance are dead after being treatment with formaldehyde with the same concentration. Meanwhile, an Annexin V-FITC cell apoptosis detection kit is used for detecting apoptosis of cells treated with formaldehyde and the protection action of the puer tea. Clearly proved by test data, the puer tea can efficiently prevent death of the normal cells, caused by the formaldehyde, and discloses the control effect of the puer tea to the formaldehyde biological damage.

Description

technical field [0001] The present invention relates to the use of Pu'er tea, more specifically, the present invention relates to the application of Pu'er tea in eliminating the biological hazard of formaldehyde. Background technique [0002] Formaldehyde (formaldehyde, FA) is a colorless irritating gas. Because formaldehyde is widely used in human production and life, formaldehyde has become one of the harmful chemicals that human beings often encounter. Formaldehyde production industries, wood processing industries, hospital systems, food processing industries, and even ordinary home decoration are all at risk of exposure to high concentrations of formaldehyde. Therefore, the pollution of formaldehyde and its biological toxicity to human beings have attracted more and more widespread attention, and how to prevent the biological hazards of formaldehyde has also become a topic of widespread concern. [0003] In June 2004, IARC (The International Agency for Research on Canc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23F3/18
Inventor 罗瑛盛军司晓宇唐文如
Owner 普洱市人民政府茶产业发展办公室科技服务中心
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