Artificial rearing method of larvae of beet armyworm

A technology of beet armyworm and artificial feed, which is applied in animal husbandry and other fields, can solve the problems of low reliability of research results, low pupation rate and weight of pupae, low survival rate of larvae, etc. Simple method and high larval survival rate

Inactive Publication Date: 2012-07-04
SHANDONG AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

(1) The survival rate of larvae is low, and the reliability of the research results is not high in the bioassay of insecticide pests
(2) The pupation rate and pupae weight are low, which affects the reproduction of offspring beet armyworm, which in turn affects the number and health of the subcultured test insects
(3) The feeding method is cumbersome, labor-intensive and time-consuming

Method used

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  • Artificial rearing method of larvae of beet armyworm
  • Artificial rearing method of larvae of beet armyworm

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Example 1: about 200 beet armyworm egg masses collected from the green onion field in Zhangqiu, Shandong in 2009 were raised indoors with the method of the present invention.

[0017] (1) After the eggs are sterilized, they are placed in an incubation tank, and fresh cabbage leaves are placed in the tank. Finally, 153 newly hatched larvae were obtained, and the hatching rate was 76.50%.

[0018] (2) Take 96 newly hatched larvae hatched on the same day, and insert them into the 24-hole insect culture plate; artificial feed is housed in the 24-hole insect culture plate, and 4 newly hatched larvae in each hole are raised to the 3rd instar 92 larvae were obtained, and the survival rate of the 3rd instar larvae was 95.83%.

[0019] (3) Randomly select 50 larvae of the 3rd instar of Spodoptera exigua, transfer to the flat-bottomed glass finger tube, artificial feed is housed in the finger tube, 2 larvae in every tube, raise to pupation, the number of pupation is 47, The pup...

Embodiment 2

[0021] Embodiment 2: Take the beet armyworm eggs obtained by subculture feeding as experimental materials, and raise them according to the method of the present invention. Assuming reference to the rearing method: fresh young cabbage leaves are not placed during the hatching process of the eggs, and the newly hatched larvae are transferred to flat-bottomed glass finger tubes and raised to pupate, and other details are the same as the present invention. Both feeding methods were set up with 3 replicates.

[0022] (1) The rearing method of the present invention: get the egg mass of the same day and place it in the hatching tank after being sterilized, place fresh cabbage leaves in the tank, count and obtain the number of newly hatched larvae, and calculate the hatching rate; choose the newly hatched larvae hatched on the same day, In the 24-hole insect culture plate, artificial feed is housed in the 24-hole insect culture plate, 4 newly hatched larvae in each hole, the 3rd insta...

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Abstract

The invention discloses an artificial rearing method of larvae of beet armyworm, and the method comprises the following steps: (1) placing eggs of the beet armyworm in a ground glass hatching jar, placing fresh young cabbage leaves after surface disinfection by 75% of alcohol in the jar and sealing by using three-layer gauze till hatching; (2) selecting the freshly hatched larvae of the beet armyworm into a 24-hole insect rearing plate loaded with artificial feed, and rearing till 3 ages; and (3) transferring the 3-age larvae of the beet armyworm into flat bottom glass finger-shaped pipes loaded with the artificial feed, enabling each pipe to contain two larvae, and rearing till pupation. The method is simple, the rearing process occupies small space, the survival rate of the larvae is high, and a large number of standard test insects can be obtained.

Description

(1) Technical field [0001] The invention relates to insect feeding technology, in particular to an artificial feeding method for beet armyworm larvae. (2) Technical background [0002] Beet armyworm (Spodoptera exigua) belongs to Lepidoptera, Noctuidae, is a worldwide distribution of agricultural pests, it can damage more than 170 kinds of plants. Since the 1990s, it has occurred in various provinces in the north and south of my country, mainly damaging cruciferous vegetables, and also causing serious damage to green onions, asparagus, cotton, soybeans, and peanuts. Beet armyworm likes high temperature, and its main damage periods are mostly concentrated in high temperature periods in various places. In my country, with the increase of latitude, the annual generation of beet armyworm gradually decreases. Such as Hebei, Henan, Beijing, Shandong, Anhui, Jiangsu and other northern regions from July to September (2 to 4 generations); Shanghai and Hunan from June to October (3 ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
Inventor 慕卫王伟
Owner SHANDONG AGRICULTURAL UNIVERSITY
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