Method for preparing precocious white needle mushroom strain

A technology of white Flammulina velutipes and yellow golden needles, applied in botany equipment and methods, horticulture, applications, etc., can solve the problems of long cultivation period of white Flammulina velutipes, deepening of the base of the stipe, early fruiting, etc.

Inactive Publication Date: 2012-11-14
INST OF SOIL FERTILIZER SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many types of Flammulina velutipes, and the taste, body color and cultivation cycle of different Flammulina velutipes are different. The common ones are yellow Flammulina velutipes and white Flammulina velutipes. Pale, light yellow, deep in the lower part, golden to dark brown, early fruiting, short cultivation period, but if the management method is not proper, it is

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 , a kind of creation method of precocious white Flammulina velutipes bacterial strain, comprises the following steps:

[0030] (1) Monokaryon isolation and identification, the operation method is as follows:

[0031] a, spore collection, take out the mature fruiting body cap of Flammulina velutipes strain "Golden Silk", place it on sterile paper, and place it for 20 hours until spore prints appear on the sterile paper.

[0032] b. Spore germination culture, remove the spore print on the sterile paper and put it in sterile water to make a spore liquid, then dilute the spore liquid to 80 / ml with sterile water, take 1 ml of the spore liquid and coat the medium plate Above, the culture medium is yeast powder glucose agar medium, cultivated at 25°C for 5 days, until the spores germinate into white colonies, take a single white colony and transfer to yeast powder glucose medium, and cultivate at 25°C.

[0033] c. Monokaryon identification: take the mycelium and pl...

Embodiment 2

[0050] Example 2, A kind of creation method of precocious white Flammulina velutipes bacterial strain, comprises the following steps:

[0051] (1) Monokaryon isolation and identification, the operation method is as follows:

[0052] a. Spore collection, take out the mature fruiting body cap of Flammulina velutipes strain "Golden Silk", place it on sterile paper, and place it for 24 hours until spore prints appear on the sterile paper.

[0053] b. Spore germination culture, remove the spore print on the sterile paper and put it into sterile water to make a spore liquid, then dilute the spore liquid to 100 / ml with sterile water, and take 1 ml of the spore liquid to coat the medium plate Above, the culture medium is yeast powder glucose agar medium, cultured at 28°C for 4 days, until the spores germinate into white colonies, and a single white colony was transferred to yeast powder glucose agar medium, and cultured at 28°C.

[0054] c. Monokaryon identification: take the mycel...

Embodiment 3

[0071] Example 3 , a kind of creation method of precocious white Flammulina velutipes bacterial strain, comprises the following steps:

[0072] (1) Monokaryon isolation and identification, the operation method is as follows:

[0073] a. Spore collection, take out the mature fruiting body cap of Flammulina velutipes strain "Golden Silk", place it on sterile paper, and place it for 12 hours until spore prints appear on the sterile paper.

[0074]b. Spore germination culture, remove the spore print on the sterile paper and put it into sterile water to make a spore liquid, then dilute the spore liquid to 50 / ml with sterile water, and take 1 ml of the spore liquid to coat the medium plate Above, the culture medium is yeast powder glucose agar medium, cultivated at 20°C for 6 days, until the spores germinate into white colonies, and transfer a single white colony to yeast powder glucose agar medium, and culture at 20°C.

[0075] c. Monokaryon identification: take the mycelium and...

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Abstract

The invention discloses a method for preparing a precocious white needle mushroom strain. In the method, a yellow needle mushroom strain 'gold wire' and a white needle mushroom strain F092 are used as parents; and by using a di-monad hybridization method, the white needle mushroom strain F212-1 is prepared. The method comprises the following steps of separation and identification of a mononuclearbody, hybridization matching, screening of hybrids and construction of a hybrids selfing strain. The white needle mushroom strain prepared by using the method has the following characteristics that afruit body is white and precocious; the root of prostheca has no tomenta; not only are the characters of precocity and no tomenta at the root of the prostheca of a yellow needle mushroom maintained, but also the character that the fruit body of the white needle mushroom is white is maintained; the cultivating time is shortened; and the method is suitable for production in various cultivation technical modes.

Description

technical field [0001] The invention relates to a method for creating a bacterial strain, in particular to a method for creating a precocious white Flammulina velutipes strain. Background technique [0002] Flammulina velutipes is famous all over the world for its smooth and tender cap, crisp handle, rich nutrition and delicious taste. According to the measurement, the amino acid content of Flammulina velutipes is very rich, which is higher than that of common mushrooms. The dry product of Flammulina velutipes contains 8.87% protein, 60.2% carbohydrates, and 7.4% crude fiber. Regular consumption can prevent and treat ulcers. Flammulina velutipes is not only a delicious food, It is also a better health food, and the domestic and foreign markets are increasingly broad. Flammulina velutipes is cultivated by artificial cultivation techniques, which can obtain stable and reliable output. [0003] There are many types of Flammulina velutipes, and the taste, body color and cultiva...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 王波黄忠乾甘炳成
Owner INST OF SOIL FERTILIZER SICHUAN ACAD OF AGRI SCI
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