Preparation method for burkholderia cepacia, and application in high-performance phosphate-dissolving microbial fertilizer by using the same

A Burkholderia, phosphorus-dissolving technology, applied in the direction of application, bacteria, microorganisms, etc., can solve the problems that the phosphorus-dissolving mechanism is not very clear, cannot be concluded, and is not deep enough, so as to enhance plant disease resistance and not pollute the environment , the effect of making full use of

Inactive Publication Date: 2011-10-26
JILIN ACAD OF AGRI SCI
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology helps activating solubility-dispersing elements like calcium from plants effectively without causing excessive damage or harmful chemicals during production processes. It also increases the efficiency of applying microbial fertils with high levels of nitrogen (N) content compared to traditional methods such as adding urea). Additionally, it allows farmers to use less expensive sources of nutrients while still producing effective crops due to its ability to stimulate root growth.

Problems solved by technology

This patent describes various technical problem addressed in this patents relating to improving efficiency or productivity of nitrogen fixated vegetative macrophytes such as legumes and cycling grass roots through decompositing insolvent phosporylite metabolites called phytophyllobiontic acid(PB) residues within their own root systems without causing excessive loss of beneficial ones like phosphorous. Existing methods involve adding other substances to stimulate phosphor release, resulting in decreased yields and reduced activity levels. To address this issue, efforts should focus on finding effective ways to break down insolvability complexities associated with certain mineral nutrients found naturally occurring throughout nature.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method for burkholderia cepacia, and application in high-performance phosphate-dissolving microbial fertilizer by using the same
  • Preparation method for burkholderia cepacia, and application in high-performance phosphate-dissolving microbial fertilizer by using the same
  • Preparation method for burkholderia cepacia, and application in high-performance phosphate-dissolving microbial fertilizer by using the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] (1) Screening and Identification of Efficient Phosphorus Solubilizing Microbial Strains

[0013] 1. Screening of bacterial strains

[0014] Take the soil samples for planting corn from the central part of our province, use the dilution plate separation method, take 1g of the soil samples under aseptic conditions, and dilute them to 10 with sterile water in a 10-fold gradient. - 1 ~10 - 6 For the dilution, take 0.1 mL of the dilution and spread it on a basal medium plate and incubate at 28°C, and isolate and screen 24 functional strains (Table 1).

[0015] 2. Determination of Phosphorus Solubilizing Ability of Bacterial Strains

[0016] Calcium phosphate was used as the sole phosphorus source, and the phosphorus-dissolving ability of the isolated and screened strains was determined. The result shows that the bacterial strain of the isolated bacterial strain 58.3 has stronger comprehensive ability (as figure 1 shown), the phosphorus-dissolving ability is the stronge...

Embodiment 2

[0027] (1) Collection of soil samples: The phosphorus-poor soil for planting corn was collected, put into plastic bags, and stored in a refrigerator at 4°C.

[0028] (2) Isolation and screening of strains (Burkholderia)

[0029] Screening medium: glucose 10.0 g, (NH 4 ) 2 SO 4 0.5 g, NaCl 0.3 g, KCl 0.3 g, MgSO 4 ·7H 2 O 0.3 g, FeSO 4 ·7H 2 O 0.03 g, MnSO 4 4H 2 O 0.03 g, Ca 3 (PO 4 ) 2 10.0 grams, 0.5 g yeast powder, 15 g agar powder, pH value 7.0-7.2, water 1000 mL; sterilize at 121°C for 20 minutes and then make a plate solid medium for later use;

[0030] The soil samples were subjected to 10-fold gradient dilution under sterile conditions to prepare 10 －1 ~10 －6 Then take 0.1mL samples of different dilutions and spread them on the above-made screening plate medium by the plate streak method. After culturing at 28°C for 3 days, select the colonies that produce larger transparent circles (phosphate-dissolving circles) and transfer to Received on the phosphat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method for burkholderia cepacia, and an application in high-performance phosphate-dissolving microbial fertilizer by using the same. The method is characterized in that: the method comprises the following steps: (1) collecting deficient phosphorus soil for planting corns, putting the soil in a plastic bag, and placing the plastic bag in a 4 DEG C refrigerator for storing; (2) separating and screening a (burkholderia cepacia) strain; (3) detecting phosphorus solubilizing capability of the (burkholderia cepacia) strain; (4) identifying the strain: wherein the screened strain is subjected to a DNA sequencing and a 16SrRNA phylogenetic tree construction to be identified as the burkholderia cepacia. According the present invention, activation rate of the insoluble phosphate in the soil can reach to 10.94%-13.03%, utilization rate of the P reaches to 21.75%-37.98%, and the phosphorus fertilizer amount can be reduced by one third in application for the corns. The phosphate-dissolving microbial fertilizer provided by the present invention has advantages of low cost, good effect, disease resistance of plants enhancing, no pollution on environment and fully utilization of potential phosphorus resource.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Owner JILIN ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap