New application of epigallocatechin gallate (EGCG)

A technology of epigallocatechin and gallocatechin is applied in the field of epigallocatechin gallate (EGCG), which can solve the problem that EGCG cannot be artificially synthesized.

Inactive Publication Date: 2011-11-09
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, EGCG cannot be synthesized artificially, and tea leaves are the only source

Method used

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  • New application of epigallocatechin gallate (EGCG)
  • New application of epigallocatechin gallate (EGCG)
  • New application of epigallocatechin gallate (EGCG)

Examples

Experimental program
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Embodiment Construction

[0013] 1 Culture of glioma U251 cells: the glioma U251 cell line was stored at 37°C, 5% CO 2 Constant temperature incubator. The cells in the logarithmic growth phase were digested with trypsin and detached, and then an appropriate amount of culture medium was added to facilitate the mixing of the cells; use a pipette to transfer them into a 15ml centrifuge tube, and centrifuge at 1500rpm for 3min; remove the supernatant; use 5ml sterile 1× Suspend the cells in PBS, and mix by pipetting; add about 2ml of cell suspension to a new culture bottle with 5ml of fresh medium, and place at 37°C, 5% CO 2 Cultured in an incubator, passaged once in about 3 days.

[0014] 2 MTS cell proliferation experiment: the cells in the logarithmic growth phase were digested with trypsin to remove the wall, and then an appropriate amount of medium was added to mix the cells, and then the cells were transferred to a 96-well plate, and the cell counting plate was counted at 1×10 per well. 5 cells / ml...

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Abstract

The invention relates to applications of epigallocatechin gallate (EGCG) in preparing a medicament for inducing glioma cell apoptosis and preparing a medicament for inhibiting glioma cell proliferation. Shown by experimental results, the EGCG can obviously inhibit the glioma cell proliferation (P(0.001), with the increase of dosage of the EGCG and extension of time, the cyto-inhibition is more obvious; and shown by experimental results of cell apoptosis, 48 hours later after the EGCG is added, with the increase of concentration, the number of cell apoptosis is markedly increased.

Description

technical field [0001] The invention relates to a new application of epigallocatechin gallate (EGCG). Background technique [0002] The MTS proliferation assay is a modified version of the MTT assay, CellTiter 96 ? The AQueous Non-Radioactive Cell Proliferation Assay determines the number of viable cells in cell proliferation assays using a colorimetric method. This system consists of a new formazan compound MTS and an electron coupler PMS. Dehydrogenases in metabolically active living cells convert MTS to liquid soluble formazan compounds, and the amount of formazan product as measured by absorbance at 490 nm is directly proportional to the number of viable cells in culture. [0003] In normal cells, phosphatidylserine (PS) is only distributed on the inner side of the lipid bilayer of the cell membrane, but in the early stage of apoptosis, the phosphatidylserine (PS) in the cell membrane is turned from the inside to the outside of the lipid membrane. Annexin V has a hig...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): A61K31/353A61P35/00
Inventor李红宋一超陈付学
OwnerSHANGHAI UNIV