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Method for breeding cattle through polymorphism of angiopoietin-related protein 4 (ANGPTL4) gene

A technology of genetic polymorphism and genetics, applied in the direction of animal husbandry, to achieve the effect of promoting rapid development

Inactive Publication Date: 2011-11-16
XINYANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

At present, its research is mainly on two aspects of blood vessels and inhibition of cell growth, which is still in its infancy.

Method used

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  • Method for breeding cattle through polymorphism of angiopoietin-related protein 4 (ANGPTL4) gene
  • Method for breeding cattle through polymorphism of angiopoietin-related protein 4 (ANGPTL4) gene
  • Method for breeding cattle through polymorphism of angiopoietin-related protein 4 (ANGPTL4) gene

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Embodiment Construction

[0032] The present invention will be described in detail below in conjunction with specific embodiments.

[0033] 1 Primer design and synthesis

[0034] Referring to the bovine LXRa gene (accession number: NC_007313) and ANGPTL4 gene (accession number: NC_007305) sequences published by GenBank, the primers were designed using Primer Premier 5.0 software and sent to Nanjing GenScript for synthesis. The specific information is shown in Table 1.

[0035] Table 1 Information about the primers of bovine LXRa gene and ANGPTL4 gene

[0036] Table 1 Primers information of bovine LXRa and ANGPTL4 gene

[0037]

[0038]

[0039] 2 primer dilutions

[0040] Briefly centrifuge the primer tube at 8000r / min for 1min, add an appropriate amount of sterilized double-distilled water according to the primer list to prepare a stock solution with a concentration of 100μM, and dilute it to 10μM when used. The stock solution was stored in a -20°C refrigerator for later use, and the diluted ...

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Abstract

The invention discloses a method for breeding cattle based on the polymorphism of an angiopoietin-related protein 4 (ANGPTL4) gene. Cattle with a specific ANGPTL4 gene are selected as male parents and / or female parents to be bred. The method is characterized by the 1422 locus sequence TT of the ANGPTL4 gene. By the method, cattle with better indexes such as body height, body inclined length, hip width and the like can be bred, and the rapid development of the beef cattle breeding industry in China is promoted.

Description

technical field [0001] The invention relates to a cattle breeding method, in particular to a cattle breeding method through the polymorphism of the bovine ANGPTL4 gene. Background technique [0002] Beef meat is delicious, nutritious, and the protein content is higher than that of ordinary meat. It not only contains all the amino acids necessary for human beings, but also has the advantages of low cholesterol and low fat. It is a good product among human meat products. Since the 1960s, the demand for beef in the international market has continued to grow, and the world's beef cattle industry has developed rapidly, and my country has become the third largest beef producer in the world after the United States and Brazil. With the continuous improvement of people's living standards and the improvement of meat structure, the consumption of beef shows a continuous growth situation. At present, my country still needs to import a large amount of high-grade beef every year. High-qu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A01K67/027
Inventor 马云李荣荣李芬侯飞王启钊白芳张华丽王新庄陈宁博杨婷
Owner XINYANG NORMAL UNIVERSITY
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