Frog skin extract and its extraction method

An extraction method and extract technology are applied in the field of frog skin extract and its extraction, which can solve the problems of low content of frog collagen and low yield, and achieve the effects of lightening pigmentation, brightening skin tone and reducing wrinkles.

Active Publication Date: 2015-07-29
JILIN HONGJIU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The yield of the current method for extracting frog collagen is not high, and the content of frog collagen obtained is low

Method used

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  • Frog skin extract and its extraction method
  • Frog skin extract and its extraction method
  • Frog skin extract and its extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation of embodiment 1 frog skin extract

[0027] Take 100g of dried wood frog skin, soak it in 0.5% sodium hydroxide alkali solution, soak until softened, freeze-dry at -75°C for 6 hours, crush it into 40 mesh coarse powder, add 8 times the amount of water, stir and heat to 60°C , keep warm for 1 hour, cool to 37°C, add acetic acid to acidify, adjust the pH value to 3, stir evenly, keep warm at 37°C for 15 hours, adjust the pH value to 3, add 0.5g pepsin (enzyme activity is 100,000 IU / g) Mix with 1.0 g of cellulase (enzyme activity is 1.4 million IU / g), stir evenly, carry out biological enzymolysis for 12 hours, heat to 60°C to inactivate the enzyme, centrifuge at 3000 rpm at high speed, take the supernatant, and use 10% sodium carbonate solution to adjust the pH value to 6-7, add saturated ammonium sulfate solution for salting out until no precipitate precipitates, filter and collect the precipitate, add purified water to the precipitate to dissolve it, put i...

Embodiment 2

[0032] The preparation of embodiment 2 frog skin extract

[0033]Take 100g of dried wood frog skin, soak it in 0.5% sodium hydroxide alkali solution, soak until softened, dry at -78°C for 4 hours, grind it into 20 mesh coarse powder, add 10 times the amount of water, stir and heat to 58°C, Keep warm for 1 hour, cool to 38°C, add hydrochloric acid to acidify, adjust the pH value to 2, stir evenly, keep warm at 38°C for 12 hours, adjust the pH value to 2, add 1.0g pepsin (enzyme activity is 100,000 IU / g) and 1.2g of cellulase (enzyme activity is 1.4 million IU / g), stir evenly, carry out biological enzymolysis for 15 hours, heat to 60°C to inactivate the enzyme, centrifuge at 3000 rpm at high speed, take the supernatant, and use 10 % sodium hydroxide solution to adjust the PH value to neutral, add saturated ammonium sulfate solution to carry out salting out until no precipitate separates out, filter and collect the precipitate, add purified water to the precipitate to dissolve it...

Embodiment 3

[0038] The preparation of embodiment 3 frog skin extract

[0039] Take 100 g of dried wood frog skin, soak it in 0.5% sodium hydroxide alkali solution, soak until softened, dry at -76°C for 5 hours, crush it into 30 mesh coarse powder, add 9 times the amount of water, stir and heat to 55°C, Keep warm for 1 hour, cool to 37.5°C, add acetic acid to acidify, adjust the pH value to 2, stir evenly, keep warm at 37.5°C for 14 hours, adjust the pH value to 2, add 0.8g pepsin (enzyme activity is 100,000 IU / g) and 1.5 g of cellulase (enzyme activity is 1.4 million IU / g), stirred evenly, carried out biological enzymolysis for 14 hours, heated to 60°C to inactivate the enzyme, centrifuged at 3000 rpm at high speed, took the supernatant, and used 10 % sodium carbonate solution to adjust the PH value to neutral, add saturated ammonium sulfate solution and carry out salting out until no precipitate separates out, filter and collect the precipitate, add purified water to the precipitate to d...

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Abstract

The invention relates to the field of biological technology, in particular to an extraction method for a frog skin extract. According to the method, the frog skin extract can be effectively obtained by a biological enzymolysis technology, wherein the purity of frog collagen reaches 54.6 to 56.2 percent. The obtained frog skin extract can effective treat gastritis, enteritis and dysentery and can effectively reduce hypertension, wherein the effective rate of treating chloasma reaches 95.0 percent; the effective rate of improving fine wrinkle reaches 97.5 percent; and the effective rate of improving dark yellow skin reaches 96.0 percent.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a frog skin extract and an extracting method thereof. Background technique [0002] Collagen is a kind of sugar-containing protein, which is widely distributed in nature, mainly in the connective tissue of animals, as the constituent materials of skin, bones, tendons, ligaments, and blood vessels; about 16% of human body components are proteins, Collagen accounts for 30-40% of the total protein in the body, and is the most abundant protein in the human body; collagen is rich in amino acids such as glycine, proline, and hydroxyproline needed by the human body. Collagen is one of the main components of the human tissue structure, and it is also the most abundant protein in the human body, accounting for about 1 / 3 of the total protein in the human body, equivalent to 6% of the body weight. It spreads all over the tissues and organs of the body, such as : Skin, bone, cartilage, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06A61K38/39A61K38/03A61P31/04A61P29/00A61P1/04A61P1/12A61P1/00A61P9/12A61P17/00
CPCY02A50/30
Inventor 冯涛金爱玉白凤英
Owner JILIN HONGJIU BIO TECH
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