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Method for producing seed source of Cordyceps militaris with high-yield cordycepin by radiation breeding

A technology of Cordyceps militaris and radiation breeding, applied in the field of genetic breeding of edible fungi and medicinal fungi, can solve the problems of limited improvement range and unsatisfactory effect, and achieve the effect of broad application prospects

Inactive Publication Date: 2012-07-25
SHENYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In order to solve the current technical problem of increasing the cordycepin content of Cordyceps militaris by adding a certain amount of protein sources to the culture medium, the improvement range is limited and the effect is not ideal, and a kind of Cordycepin militaris high-yielding cordycepin is provided for radiation breeding. The production method of the provenance of Cordyceps sinensis is obtained by screening after low-dose cesium 137 radioactive element radiation, named: ycc Gy 1016; the specific production method is realized through the following steps:

Method used

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  • Method for producing seed source of Cordyceps militaris with high-yield cordycepin by radiation breeding

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Embodiment 1

[0036] What this patent application protects is a production method of a new variety of Cordyceps militaris that can increase the content of cordycepin. This variety is obtained by screening after low-dose cesium 137 radioactive element radiation, named: ycc Gy1016.

[0037] The specific implementation steps are:

[0038] Firstly, the Cordyceps militaris variety ycc-01, which is widely used in production, is selected as the starting strain for radiation breeding;

[0039] Use the following medium formula for ycc-01: 20 grams of glucose, 5 grams of peptone, 3 grams of yeast extract powder, 3 grams of potassium dihydrogen phosphate, 2 grams of magnesium sulfate, 100 mg of multivitamins and 1000 ml of distilled water, and produce liquid by conventional methods Provenance;

[0040] Then prepare enriched PDA medium according to the conventional method, take a 10 cm culture dish in diameter and sterilize it synchronously by autoclaving, pour 0.3 milliliters of liquid provenance ba...

Embodiment 2

[0053] The production method of radiation breeding Cordyceps militaris provenance with high yield of cordycepin, firstly select the Cordyceps militaris variety ycc-01 widely used in production as the starting strain of radiation breeding;

[0054] Use the following medium formula for ycc-01: 20 grams of glucose, 5 grams of peptone, 3 grams of yeast extract powder, 3 grams of potassium dihydrogen phosphate, 2 grams of magnesium sulfate, 100 mg of multivitamins and 1000 ml of distilled water, and produce liquid by conventional methods Provenance;

[0055] Then prepare enriched PDA medium according to the conventional method, take a 10 cm culture dish with a diameter of 10 centimeters and sterilize it synchronously by autoclaving, pour 0.3 milliliters of liquid provenance bacterium liquid containing conidia diluted 3000 times after cooling, and spread evenly on On the plate, colonize and culture at 18-25°C in the dark for 24-36 hours, and then transfer to radiation treatment;

...

Embodiment 3

[0059] The production method of radiation breeding Cordyceps militaris provenance with high yield of cordycepin, firstly select the Cordyceps militaris variety ycc-01 widely used in production as the starting strain of radiation breeding;

[0060] Use the following medium formula for ycc-01: 20 grams of glucose, 5 grams of peptone, 3 grams of yeast extract powder, 3 grams of potassium dihydrogen phosphate, 2 grams of magnesium sulfate, 100 mg of multivitamins and 1000 ml of distilled water, and produce liquid by conventional methods Provenance;

[0061] Then prepare enriched PDA medium according to the conventional method, take a 10 cm culture dish with a diameter of 10 centimeters and sterilize it synchronously by autoclaving, pour 0.3 milliliters of liquid provenance bacterium liquid containing conidia diluted 3000 times after cooling, and spread evenly on On the plate, colonize and culture at 18-25°C in the dark for 24-36 hours, and then transfer to radiation treatment;

...

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Abstract

A method for producing a seed source of Cordyceps militaris with high-yield cordycepin to improve the yield of the cordycepin is designed to solve the technical problem that improvement on the content of cordycepin in Cordyceps militaris by adding a certain amount of protein sources into culture medium is low and unsatisfactory. The method is used for breeding target strains by radiation, induced mutation and screening. A target strain ycc Gy 1016 is obtained by subjecting an original strain ycc-01 to characteristic parameter radiation treatment, subjecting a varied colony to screening and purification, scientifically testing indicators of generated cordycepin and estimating culture stability, and accordingly the method for producing new strains of Cordyceps militaris with high-yield cordycepin is achieved. Testing show that cordycepin productivity of the strain ycc GY 1016 is 3.8 times of that of the original strain ycc-01. The method has important value on artificial production of functional Cordyceps militaris and has bright application prospect.

Description

technical field [0001] The invention relates to a production method of an edible fungus seed source, in particular to a production method of a Cordyceps militaris seed source for radiation breeding with high yield of cordycepin. The invention belongs to the field of genetic breeding of edible fungi and medicinal fungi. Background technique [0002] Cordyceps sinensis [Cordyceps sinensis] is a traditional precious medicinal material in my country. It is distributed in Tibet, Qinghai, Sichuan, Gansu, Yunnan and other places, and has special pharmacological and therapeutic effects. It is also a good medicine and food tonic. Among them, cordycepin is the most unique and important medicinal ingredient. [0003] In recent years, due to the expansion of human activities and the deterioration of the ecological environment, wild Cordyceps sinensis has continued to decrease, and the price has also continued to soar. By 2010, high-quality Cordyceps had been sold to 160,000 yuan / kg (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04A01H1/06
Inventor 王升厚徐方旭刘诗扬李锋董忠生王俊伟
Owner SHENYANG NORMAL UNIV
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