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Stem Accelerated Isothermal Nucleic Acid Amplification Technology

A nucleic acid and amplicon technology, applied in the field of nucleic acid amplification, can solve problems such as differences, and achieve the effect of increasing the amplification speed and reducing the experimental cost.

Active Publication Date: 2016-09-28
LUMORA LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The properties of the amplicons produced vary widely depending on the nucleic acid amplification technique performed

Method used

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  • Stem Accelerated Isothermal Nucleic Acid Amplification Technology
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  • Stem Accelerated Isothermal Nucleic Acid Amplification Technology

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Embodiment Construction

[0051] The present invention provides an improved polynucleic acid amplification method.

[0052] Therefore, in one embodiment, the present invention provides a kind of method for synthesizing polynucleic acid, and this method comprises the steps:

[0053] a) providing a target template comprising at least a first and a second reciprocating primer binding region;

[0054] b) providing a first primer comprising first and second fragments, wherein said first fragment is substantially complementary to a first reciprocal primer binding region on said template, and said second fragment comprises a sequence substantially is complementary to another region in the first primer or to a region in an amplicon generated by the first segment of the first primer, thereby enabling the second segment to form a circle;

[0055] c) providing a second primer comprising a first segment and an optional second segment, wherein the first segment is substantially complementary to a second primer bin...

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Abstract

The invention relates to the technical field of nucleic acid amplification. In particular, the present invention relates to stem-accelerated isothermal nucleic acid amplification techniques. The present invention relates to a method using a stem primer which improves rapid and specific amplification of a test sample.

Description

technical field [0001] The present invention relates to a nucleic acid amplification technology, in particular to a method which improves rapid and specific amplification and detection of test samples. Background technique [0002] Nucleic Acid Amplification Technology (NAAT) is an invaluable and powerful tool in many areas of research and diagnostics. Nucleic acid amplification technology can detect and quantify the nucleic acid in the sample with high sensitivity and specificity, and can perform quantitative analysis on the nucleic acid in the sample. [0003] Nucleic acid amplification can be used to determine the presence or absence of a particular template nucleic acid in a sample, which can be judged by the presence or absence of amplification products after performing a particular nucleic acid amplification technique. Conversely, the absence of any amplification product indicates the absence of template nucleic acid in the sample. These techniques are crucial in the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6844
Inventor 劳伦斯·C·第希盖伊·柯德乐奥尔加·甘德尔曼凯瑟·J·迈克滚
Owner LUMORA LTD
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