Application of hederagenin in preparation of medicine for resisting senile dementia
An ivy saponin, senile technology, applied in the directions of drug combination, antidote, pharmaceutical formulation, etc., can solve the problems of lack of drugs and treatment methods, achieve the protective effect of cholinergic system damage, resist brain nerve damage, increase The effect of SOD vitality
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Embodiment 1
[0013] Example 1 Effect of helexin on Aβ 25-35 Induced repair effect of PC-12 cell injury model
[0014] The purpose of this experiment is to explore the anti-AD activity of hedera sapogenin in vitro.
[0015] 1. Materials and methods:
[0016] Lotion: 10g K 2 Cr 2 o 7 , 16ml H 2 O, 200ml concentrated H 2 SO 4 mixed preparation;
[0017] D-Hank's balanced salt solution: NaCl 4.00g, KCl 0.20g, NaCl 2 HPO 4 12H 2 O 0.067g, KH 2 PO 4 0.03g, NaHCO 3 0.175g, phenol red 0.01g, triple distilled water 500mL, adjust the pH to 7.0-7.4;
[0018] PBS balanced salt solution: NaCl 8.00g, KCl 0.20g, NaCl 2 HPO 4 12H 2 O 3.49g, KH 2 PO 4 0.20g, triple distilled water 1000mL, adjust the pH to 7.2~7.4;
[0019] RPMI 1640 medium: RPMI Medium 1640 dry powder medium (GIBCO Invitrogen) 10.4g, NaHCO 3 2.0g, 0.06g of penicillin (commercially available in units of 4 million U), 0.1006g of streptomycin (commercially available in units of 1 million U), prepared in 900mL of three...
Embodiment 2
[0045] Example 2 In vivo pharmacodynamics of hedera saponin against AD in vivo model of acute AD induced by scopolamine hydrobromide in mice with dementia
[0046] 1. Reagents and kits:
[0047] Hedera saponin (purity is 94.85%, the preparation method is the same as in Example 1); Scopolamine hydrobromide (purchased from Nanjing Zelang Pharmaceutical Technology Co., Ltd.);
[0048] Galantamine hydrobromide (Shaanxi Kangsheng Biotechnology Co., Ltd.);
[0049] Ginkgo biloba extract (Xuzhou Hengkai Ginkgo Products Co., Ltd.);
[0050] Coomassie Brilliant Blue Protein Assay Kit (Nanjing Jiancheng Bioengineering Institute);
[0051] Acetylcholinesterase Assay Kit (Nanjing Jiancheng Bioengineering Institute);
[0052] Superoxide dismutase test kit (Nanjing Jiancheng Bioengineering Institute);
[0053] Mouse β-amyloid 1-40 enzyme-linked immunoassay kit (Shanghai Jianglai).
[0054] 2. Animals
[0055] ICR mice, half male and half female, weighing 18-22g (provided by Qinglong M...
Embodiment 3
[0143] Example 3 Take the hedera saponin prepared according to the method of Example 1, add common excipients for capsules, and prepare capsules according to a conventional preparation process.
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