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Establishing method for indoor symbiotic relationship of amanita flavipes and cyclobalanopsis glaucoides as symbiotic plant

A technology of symbiotic relationship and establishment method, applied in the biological field, can solve problems such as difficulty in development and utilization, incapability of artificial cultivation, etc., and achieve the effect of fast growth

Inactive Publication Date: 2013-09-04
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of Amanita belong to ectomycorrhizal fungi, which cannot be artificially cultivated at present, and are difficult to develop and utilize. The growth and development of its fruiting bodies are closely related to symbiotic plants. Therefore, the biological relationship between Amanita and its symbiotic plants is very important. Research is a feasible way to further explore the mechanism of Amanita fruiting bodies

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] Pick mature fruiting bodies with good growth, no pests, unopened or incompletely opened umbrellas, thick stipe, and thick caps in the field; bring the fruiting bodies back to the laboratory, use alcohol cotton balls on the ultra-clean table, lightly Wipe the surface of the cap and the stipe and other parts, remove the surface sand or soil, divide the fruit body into four parts from the middle of the cap, use a scalpel to take the internal tissue block at the junction of the stipe and the cap, and cut it into about 0.08~ 0.16cm 2 small pieces; gently embed the above cut small tissue pieces into the test tube slant induction medium (potato 200.00g / L, glucose 20.00g / L, NH 4 NO 3 0.30g / L, glutamic acid 0.32 g / L, V B1 0.10mg / L, wort juice 150 ml / L, agar 11.00g / L, control the pH value to about 5.4) on the surface, culture temperature is 22~23℃, dark culture for 14 days, very few white bacteria began to germinate around the bacteria block Silk, 60 days, the tissue blocks ...

example 2

[0021] Pick mature fruiting bodies with good growth, no pests, unopened or incompletely opened umbrellas, thick stipe, and thick caps in the field; bring the fruiting bodies back to the laboratory, use alcohol cotton balls on the ultra-clean table, lightly Wipe the surface of the cap and the stipe and other parts, remove the surface sand or soil, divide the fruit body into four parts from the middle of the cap, use a scalpel to take the internal tissue block at the junction of the stipe and the cap, and cut it into about 0.08~ 0.16cm 2 small pieces; gently embed the above cut small tissue pieces into the test tube slant induction medium (potato 200.00g / L, glucose 20.00g / L, NH 4 NO 3 0.30g / L, glutamic acid 0.32 g / L, V B1 0.10mg / L, wort juice 150 ml / L, agar 11.00g / L, control the pH value to about 5.4) on the surface, culture temperature is 22~23℃, dark culture for 14 days, very few white bacteria began to germinate around the bacteria block Silk, 60 days, the tissue blocks ...

example 3

[0025] Pick mature fruiting bodies with good growth, no pests, unopened or incompletely opened umbrellas, thick stipe, and thick caps in the field; bring the fruiting bodies back to the laboratory, use alcohol cotton balls on the ultra-clean table, lightly Wipe the surface of the cap and the stipe and other parts, remove the surface sand or soil, divide the fruit body into four parts from the middle of the cap, use a scalpel to take the internal tissue block at the junction of the stipe and the cap, and cut it into about 0.08~ 0.16cm 2 small pieces; gently embed the above cut small tissue pieces into the test tube slant induction medium (potato 200.00g / L, glucose 20.00g / L, NH 4 NO 3 0.40g / L, glutamic acid 0.28 g / L, V B1 0.10mg / L, wort juice 150 ml / L, agar 11.00g / L, control the pH value to be about 5.4) on the surface, culture temperature is 22~23℃, dark culture for 16 days, very few white bacteria began to germinate around the bacteria block Filament, 55 days, the tissue ...

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Abstract

The invention relates to an establishing method for indoor symbiotic relationship of amanita flavipes and cyclobalanopsis glaucoides which is a symbiotic plant, and belongs to the field of biotechnology (plant tissues and Fungus cultivation). An overwhelming majority of amanita belongs to ectomycorrhizal fungi, can not be yet subjected to artificial cultivation at present, and can be hardly developped and utilized; and growth and development of sporocarps of the amanita has a close relationship with cyclobalanopsis glaucoids which is a symbiotic plant. The establishing method is characterized in that myceliums are induced through the sporocarps of amanita flavipes for reproduction; aseptic seedlings and potted seedlings are successfully obtained by using seeds of cyclobalanopsis glaucoides, collected in the field; reproduced myceliums are inoculated to the roots of the aseptic seedlings and the potted seedlings respectively; the result shows that inoculated seedlings are more exuberant than the non-inoculated seedlings and have more leaves, the leaves of inoculated seedlings are dark green, disease resistance of inoculated seedlings are reinforced; and therefore, the indoor symbiotic relationship of amanita flavipes and cyclobalanopsis glaucoides is innovatively established, and a technological reserve is provided for future researches on symbiotic mechanism and mechanism generated in the sporocarps of amanita.

Description

technical field [0001] The invention relates to a method for establishing an indoor symbiotic relationship between Amanita amanita and the symbiotic plant Amanita dianthus, and belongs to the field of biotechnology, in particular, it belongs to the category of plant tissue culture and large fungus mycelium culture. Background technique [0002] Amanita ( Amanita ) belongs to Basidiomycotina, Phytomycetes, Agaricales, Amanitaceae ( Amanitaceae ), is a more special and more valuable worldwide widespread genus among poisonous macrofungi. Most species of Amanita are well-known highly toxic bacteria, mainly containing amanitin. Amanitin has potential applications in the development of new drugs such as antibacterial, antiviral, antitumor, and sedative anesthesia, so it has become a research hotspot in recent years. . [0003] Most of Amanita belong to ectomycorrhizal fungi, which cannot be artificially cultivated at present, and are difficult to develop and utilize. The growt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00A01H4/00A01G7/06
Inventor 李宗菊张曦予何隽李彪周文吴鹏赵昱冯辽辽
Owner YUNNAN UNIV
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