Artificial cross breeding method for callicarpa eucallicarpa plant

A technology of artificial hybridization and Amethyst genus, which is applied in botany equipment and methods, plant gene improvement, application, etc., can solve the problems of hybrid breeding of Amethyst genus plants, achieve easy promotion, improve hybridization efficiency, and improve fruit setting rate effect

Inactive Publication Date: 2012-10-24
武汉市林业果树科学研究所
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Problems solved by technology

[0003] At present, there is no literature report related to the h

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  • Artificial cross breeding method for callicarpa eucallicarpa plant

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Embodiment 1

[0020] In July 2010, the blooming period of Baitangzi ( C. dichotorna )× Old Crow Paste ( C. giraldii Hesse ex Rehd.) artificial hybridization (Table 1).

[0021] Pollen collection: On July 2, 6 and 21, the inflorescences of the old crow paste were selected respectively, wrapped in sulfuric acid paper bags, and sealed with a stapler. Open the paper bag before pollination at 10:00-11:00 on the 3rd, 7th and 22nd, and pick the inflorescences that are loosening the pollen for later use.

[0022] Flower emasculation: On the day of hybridization on July 3, 7 and 22, select the inflorescences of Begonia japonica the day before the opening, and keep only about 20 florets in each inflorescence, and remove the rest. Carefully poke the petals with a dissecting needle to remove the anthers. At this time, the petals are in an open state and the stigma is exposed.

[0023] It should be noted that the inflorescence on the day before opening is characterized by the fact that most of the ...

Embodiment 2

[0028] The old crow paste ( C. giraldii ) × Baitangzi ( C. dichotorna ) artificial hybridization.

[0029] Pollen collection: Select the inflorescences of Begonia japonica the day before opening on July 8, wrap them in sulfuric acid paper bags, and seal them with a stapler to prevent pollen from mixing. Open the paper bag before pollination at 10:00-11:00 on July 9, and pick the inflorescences that are loosening the pollen for later use.

[0030] Flower emasculation: On the day of pollination on July 9, select the inflorescences of Laoyazi the day before opening, and only keep about 20 small flowers in each inflorescence, and remove the rest. Carefully poke the petals with a dissecting needle to remove the anthers. At this time, the petals are in an open state and the stigma is exposed.

[0031] It should be noted that the inflorescence on the day before opening is characterized by the fact that most of the flower buds are lavender, oval, and the top is loose but the tepa...

Embodiment 3

[0034] During the full flowering period from July 13th to 15th, 2011, Baitangzi ( C. dichotorna ) × Du Honghua ( C. formosana Rolfe) artificial hybridization.

[0035] Pollen collection: On July 13, select the inflorescences of Duhong flowers the day before opening, wrap them in sulfuric acid paper bags, and seal them with a stapler to prevent pollen from mixing. Open the paper bag before pollination at 10:00-11:00 on the 14th, and pick the inflorescences that are loosening the pollen for later use.

[0036] Flower emasculation: On the day of hybridization on July 14, select the inflorescences of Begonia japonica the day before opening, and keep only about 20 florets in each inflorescence, and remove the rest. Carefully poke the petals with a dissecting needle to remove the anthers;

[0037] It should be noted that the inflorescence on the day before opening is characterized by the fact that most of the flower buds are lavender, oval, and the top is loose but the tepals a...

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Abstract

The invention discloses an artificial cross breeding method for a callicarpa eucallicarpa plant. The method comprises choosing male parent inflorescence one day prior to flowering to perform bagging and collect pollen one day prior to cross breeding, at 10 to 11 on the morning of a sunny day at a full-bloom stage of July; choosing female parent inflorescence one day prior to flowering on the day of cross breeding to perform castration, continuously performing pollination twice on the same day and next day, and performing bagging; and removing bags 15 days after pollination, enabling mesh bags to be sleeved on fruits on November to avoid fruit abscission, harvesting the fruits on December, stripping out seeds, and storing the seeds below 4 DEG C. According to the artificial cross breeding method, cross breeding efficiency and fruit set percentage are effectively improved, more cross breeding progeny can be obtained, and garden seed breeding of the callicarpa plant is promoted.

Description

technical field [0001] The invention relates to the technical field of plant cross-breeding, in particular to an artificial cross-breeding method for the plants of the genus Auranthus genus Auranthus. Background technique [0002] Artificial cross-breeding is an important way to improve plant genetic characteristics and cultivate new varieties or types. Verbenaceae ( Verbenaceae ) Amethyst ( Callicarpa ) There are more than 190 species of plants, 46 species in my country, mainly distributed in the provinces south of the Yangtze River. According to the length of filaments and the way of anther dehiscence, the genus is divided into the group of true purple pearls ( Eucallicarpa ) and capping drug group ( Verticirima ). This genus of plants has various activities such as analgesic, anti-infection, anti-oxidation, and anti-tumor. In addition, most species of plants in this genus have bright flowers and fruits, large fruit quantity, and long fruit-hanging period. They ar...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 许林陈法志戢小梅谢焰锋童俊陈卫东周媛郭彩霞谭庆董艳芳徐冬云杨守坤童正富徐洪亮
Owner 武汉市林业果树科学研究所
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