Clonal tissue culture breeding method of Liquidambar formosana hance

Abstract

The invention relates to a clonal tissue culture breeding method of Liquidambar formosana Hance, belonging to the technical field of tree tissue culture breeding. The method comprises the following steps of: sterilization of explant materials, induction of sprouts, multiplication of sprouts, rooting induction, hardening of rooted seedlings, and transplanting and management of tissue culture seedlings. Specifically, the method comprises the steps of: removing leaves of a one-year-old shoot, cutting the shoot into sections with 1-2 axillary buds respectively, sterilizing the sections, conducting induced culture to the sections by using induced culture media, conducting multiplication culture and rooting culture to new sprouts, hardening and domesticating the cultured rooted seedlings, finally transplanting the domesticated seedlings on sterilized media, and managing the transplanted seedlings. By adopting the method, the breeding is not limited by seasons, the production cost is low and the land resources are saved; and the situation of browning can be effectively avoided, the effective multiplication rate is high, the rooted seedlings grow tidily, the culture period is short, the survival rate of the transplanted tissue culture seedlings is high, the seedling cultivation period is short, the clonal breeding of the Liquidambar formosana Hance can be pushed forward and the method plays an important role in large-scale propagation and popularization of improved varieties.

Description

technical field [0001] The invention relates to a method for tissue culture and breeding, in particular to a method for tissue culture and breeding of sweetgum cedar clones, and belongs to the technical field of tree tissue culture and breeding. Background technique [0002] Liquidambar ( Liquidambar formosana Hance) is a tree species of the genus Liquidambar in the family Hamamelis family. Large deciduous tree, up to 40m, with a maximum DBH of more than lm. Trunk straight; much branched, crown conical. Originating in my country, it is centered in the south of the Yangtze River Basin, reaching Taiwan in the east, Yunnan in the west, Hainan in the south, and Henan in the north. Wuzhishan is the most common in Hainan. Liquidambar is very suitable for building scenic forests in low mountains and hilly areas in southern my country, and towering trees are very magnificent in moist and fertile soil. It can also be planted as a shade tree in the garden. After the temperat...

AI Technical Summary

Problems solved by technology

However, the seed breeding seedlings have the following disadvantages. The first is that the seed breeding has strong seasonality, and it is difficult to store the seeds for a long time, and the germination rate drops sharply after one year; The demand for production and application, but the investment of building a seed garden is large and the cycle is long; the third is that the seedlings cannot be cultivated into clones, and the forests in production are highly differentiated and the forest phases are uneven. It is impossible to realize large-scale utilization of single plants with excellent performance after selection

[0006] The disadvantages of cutting breeding of sweetgum sweetgum are: first, the survival rate of old tree spikes is low, so it is necessary to build a spike garden for cuttings, after pruning and dwarfing, cultivate a suitable crown shape, and produce more spikes

The ear-picking nursery needs fine-grained management, and a lot of labor must be invested. Today, when labor costs are rising day by day, the management cost of the ear-picking garden is unbearable; second, the ear-picking mother plant is easy to age, and the service life is not long. Third, the survival rate of cuttings is affected by many factors such as weather, management, substrate, rooting factor, etc., and it is often difficult to guarantee the survival rate; fourth, the price of seedlings is high, and the market is difficult to accept

[0007] With the development of tissue culture technology, researchers are trying to use tissue culture technology to study the breeding of sweetgum sweetgum. Using conventional tissue culture technology as a reference, researchers found that there are the following problems in the tissue culture of liquid sweetgum: When cultivating buds and proliferating, the browning phenomenon is serious, and the bud tip is necrotic, even withered and brown; the survival rate of the transplanted liquidambar tissue culture seedlings is low, and the seedling cultivation period is long, so the research and development of new tissue culture technology has a great impact on the growth of liquidambar sweetgum. Breeding is important

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