A molecular motor biosensor kit for detecting Enterobacter sakazakii

Abstract

The invention relates to a molecular motor biosensor kit for detecting Enterobacter sakazakii, belonging to the field of quick detection of animal-derived food microbes. The invention mainly solves the problem of overlong time (5-6 days) in the traditional detection method. The core technique is to utilize an F0F1-ATPase molecular motor biosensor on chromatophore; and since the F0F1-ATPase can quickly rotate, coupling between the catalytic site and the proton transfer is established through the rotation. An ITS probe is connected to an epsilon subunit of the ATPase; after a sample to be detected and a negative control are respectively combined with the biosensor, the ATP synthesis amounts under the catalytic action after 10 minutes are compared; and the ATP synthesis amount can be measured according to the amount of H<+> in the environment, and the amount of H<+> is acquired according to the fluorescence intensity embodied by the F-DHPE. The kit can be used for quickly and sensitively detecting Enterobacter sakazakii in a sample to be detected at high flux.

Description

technical field [0001] The invention relates to a rapid detection technology for animal-derived food microorganisms. Background technique [0002] Traditional pathogen detection methods require non-selective enrichment, selective enrichment, separation, screening and identification for each test item. It usually takes 6 days to issue a test report, which seriously affects the quality and shelf life of the goods. Some new technologies such as PCR technology, immunological detection technology, biochip technology and other detection methods still rely on traditional detection steps, that is, they need to be enriched, which is time-consuming and labor-intensive. It usually takes several days to complete a detection. This not only increases the workload of the laboratory, but also is not conducive to the monitoring of the production process in the food industry, the quality control of the final product, and the management and control of food safety by government departments. Du...

AI Technical Summary

Problems solved by technology

[0002] Traditional pathogen detection methods require non-selective enrichment, selective enrichment, separation, screening, and identification for each inspection item. It usually takes 6 days to issue a test report, which seriously affects the quality and shelf life of the goods.

Some new technologies such as PCR technology, immunological detection technology, biochip technology and other detection methods still rely on traditional detection steps, that is, they need to be enriched, which is time-consuming and labor-intensive. It usually takes several days to complete a detection

This not only increases the workload of the laboratory, but also is not conducive to the monitoring of the production process in the food industry, the quality control of the final product, and the management and control of food safety by government departments.

Images