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Method for extracting cordyceps polysaccharide and adenosine from cordyceps sinensis mycelium
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A technology for Cordyceps polysaccharide and Cordyceps sinensis, which is applied in the field of bioengineering, can solve the problems of complex separation process, low overall yield and small separation capacity, and achieves the effects of simplified process, short separation time and reduced cost.
Inactive Publication Date: 2015-04-22
青海珍赐生物科技有限公司
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Problems solved by technology
[0005] The purpose of the present invention is to provide a method for separating polysaccharides from Cordyceps mycelium by using two-phase extraction to solve the problems of complex separation process, small separation capacity, low overall yield and high cost from Cordyceps mycelium. and adenosine method
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Embodiment 1
[0023] Take 1g of Cordyceps fungus powder in a test tube, add 40mL of water, mix well, and ultrasonically treat for 15min at 33kHZ in an ice bath, then extract in a water bath at 75°C for 3h, take 1mL of Cordyceps mycelium mixture (25g / L), Add 0.6g of potassium carbonate, then add 0.4g of ethanol, mix, stand still, and separate phases to obtain adenosine that is mainly distributed in the upper phase with a distribution coefficient of 29.5 and a recovery rate of 93.7%; polysaccharides are mainly distributed in the lower phase with a distribution coefficient of 0.07, the recovery rate was 85.6%.
Embodiment 2
[0025] Take 1g of Cordyceps fungus powder in a test tube, add 40mL of water, mix well, then in 33kHZ, ice bath condition, ultrasonic treatment for 15min, 75℃ water bath extraction for 3h, take 1mL of Cordyceps mycelium mixture (25g / L), add Add 0.3g of sodium carbonate, then add 0.4g of ethanol, mix, stand still, and separate the phases. The adenosine is mainly distributed in the upper phase, with a distribution coefficient of 49.4, and the recovery rate is 98.3%; the polysaccharide is mainly distributed in the lower phase, and the distribution coefficients are respectively 0.16, the recoveries were 68.3%.
Embodiment 3
[0027] Take 1g of Cordyceps fungus powder in a test tube, add 40mL of water, mix well, then in 33kHZ, ice bath condition, ultrasonic treatment for 15min, 75℃ water bath extraction for 3h, take 1mL of Cordyceps mycelium mixture (25g / L), add 0.4g of ammonium sulfate, then add 0.4g of ethanol, mix, stand still, and separate phases. Adenosine was mainly distributed in the upper phase with a distribution coefficient of 8.89 and a recovery rate of 84.9%; polysaccharides were mainly distributed in the lower phase with a distribution coefficient of 0.23 and a recovery rate of 87.3%.
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Abstract
The invention discloses a method for extracting cordyceps polysaccharide and adenosine from cordyceps sinensis mycelium. The method comprises the steps of (1) preparing cordyceps sinensis mycelium or extracting solution of the cordyceps sinensis mycelium into mixed liquid of the mycelium by adding water, wherein the content scope of the mycelium is 1-700 g / L; (2) adding 10-700 g of soluble inorganic salt and 10-600 g of alcohol or ketone hydrophilic organic matter into per liter of mixed liquid, prepared in the step (1), of the mycelium, and carrying out standing and phase splitting on obtained multiphase mixed liquid, wherein the upper phase is a solvent phase rich in the adenosine, and the lower phase is a salt-rich phase rich in polysaccharide; and (3) separating and obtaining the adenosine from the upper phase, and separating and obtaining the cordyceps polysaccharide from the lower phase. According to the method, a lot of defects in the conventional process for separating the polysaccharide and the adenosine from the cordyceps sinensis mycelium are overcome, the process is simplified, the separation time is short, the cost is lowered, and the method is economic and feasible.
Description
technical field [0001] The invention belongs to the technical field of bioengineering and relates to the separation technology of microbial mycelia, in particular to a method for separating cordyceps polysaccharide and adenosine from the microbial mycelium. Background technique [0002] Cordyceps inensis is a treasure of traditional Chinese medicine. It has the functions of anti-tumor, anti-virus, hemostasis and phlegm, moistening the lungs and nourishing the kidneys. At present, Cordyceps mycelium is mainly obtained through two ways: natural picking and fermented culture. Among them, the mycelium obtained by fermented culture is basically similar to natural Cordyceps sinensis in terms of its main components, pharmacological effects and clinical effects. broad attention. At present, the medicinal active ingredients in Cordyceps mycelium mainly include polysaccharides, adenosine and cordycepic acid. Among them, Cordyceps polysaccharide has the functions of improving immune ...
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